International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Determination of the Ribosomal DNA Internal Transcribed Spacers and 5.85 rDNA Sequences of Cordyceps Species

  • Bae, Jin-Sik (College of Natural Resources and Life Science, Dong-A University) ;
  • Nam Sook park (College of Natural Resources and Life Science, Dong-A University) ;
  • Jin, Byung-Rae (College of Natural Resources and Life Science, Dong-A University) ;
  • Lee, Ho-Oung (Department of Sericultural and Entomological Biology, Faculty of Agriculture, Miryang National University) ;
  • Park, Eun-Ju (Department of Life Science, Kyungnam University) ;
  • B. Tolgor (Mycological Institute, Jilin Agricultural University) ;
  • Yu Li (Mycological Institute, Jilin Agricultural University) ;
  • Lee, Sang-Mong (Department of Sericultural and Entomological Biology, Faculty of Agriculture, Miryang National University)
  • Published : 2002.09.01
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Abstract

The sequences of the internal transcribed spacers (ITS1 and ITS2) and 5.8S ribosomal DNA gene from five Cordyceps species and one Paecilomyces japonica were determined. The total length of the ITSI, 5.8S and ITS2 regions ranged from 528 to 549 bp. When the C. militaris collected from Korea was used as a standard genotype, the sequence showed 88.4%, 88.6%, 91.1% and 86.8% identity to C. pruinosa, C. sphecocephala, C. scarabaeucika and R japonica, respectively, while the lowest identity was found with C. sinensis (75.4%). Interestingly, C. sinensis was phylogenetically distant from the other Cordyceps species. To test geographic variation, furthermore, sequences of the ITS regions in the 8 samples of C. militaris collected from two localities in Korea and China analyzed and compared with the GenBank-searched sequences from Japan and China. The total length of the ITS regions of C. militaris from Korea, Japan and China was completely identical to each other with 528 bp, and the sequence divergence among three localities in pairwise comparisons ranged from 0.2% (1 bp) to 0.4% (2 bp).

Keywords

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