Successful Superovulation and Recovery of Embryos in Repeat Breeding Crossbred Cows

  • Zawar, Shyam (Raymond Embryo Research Center, Gopal Nagar-Bilaspur (Chhattisgarh), INDIA) ;
  • Mishra, U.K. (Raymond Embryo Research Center, Gopal Nagar-Bilaspur (Chhattisgarh), INDIA) ;
  • Gaswade, P.G. (Raymond Embryo Research Center, Gopal Nagar-Bilaspur (Chhattisgarh), INDIA)
  • Published : 2002.12.01

Abstract

The present study was undertaken to find out possibilities for superovulation and recovery of freezable/transferable embryo from repeat breeding crossbred cows. For this study a total of 10 Holstein crossbred cows having history of failure of conception even after more than 4~5inseminations were taken and superovulated using Folltropin-V at 100 to 140% dose schedule. The results indicated that out of 10 donors, 8 responded to superovulatory treatment and yielding a total of 94 embryos, out of which 45 were of transferable/freezable quality. The mean ovulations and mean transferable embryos were 11.5$\pm$2.91 and 4.5$\pm$1.66, respectively. These observations suggests that the repeat breeding cows having conception failure after several artificial inseminations can be used as donors for production of embryos and calves through embryo transfer technology.

Keywords

References

  1. Awasthi MK, Tiwari RP and Mishra OP. 2002. Effect of progesterone supplementation during mid luteal phase on conception in repeat breed-er crossbred cows. Indian J. Animal Reproduc-tion, 23(1): 67-68
  2. Karche SD, Dutt T and Ansari MR. 1995. A note on successful superovulation and collection of embryos from infertile donor cow. Indian J. Animal Reproduction, 16(1):66-67
  3. Nigam JM, Sood Pankaj, Vasistha NK and Singh Madhumeet. 2001. Comparative efficacy of dif-ferent drugs on superovulation and embryonic profile in cows. Indian J. Animal Reproduction, 22(1):10-13
  4. Sharma RK, Siddiqui MU and Gorani S. 1996. Successful pregnancy, superovulation and em-bryo fertility of cyclic non-breeder cow. Indian J. Animal Reproduction, 17(2): 143-144
  5. Totey SM, Gurpreet Singh, Anand RK, Gurcharan Singh, Chillar RS and Talwar GP. 1989. Suc-cessful pregnancies from cryopreserved bovine embryos transferred non-surgically to indige-nous low milk-yielding cows. Indian J. Animal Science, 59(5): 532-536
  6. Voelkel SA and Hu YX. 1992. Use of ethylene gly-col as a cryoprotectant for bovine embryos allowing direct transfer of frozen thawed embryos to recipient females. Theriogenology, 37:687-697 https://doi.org/10.1016/0093-691X(92)90148-K