Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Effects of hCG Administration on AI or Cloned Embryo Transfer of Korean Native Heifers

한우 미경산우에 있어서 인공수정 및 복제수정란 이식시 hCG 투여에 의한 수태율 향상

  • Published : 2002.12.01
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Abstract

This study was performed to investigate the improvement of the pregnancy rate of Al or clone embryo transfer on hCG administration in Korean Native heifer. A total of 42 heifers were treated with control, CIDR(withou E2 capsule). hCG after 7 day of hi, the pregnancy rate were spewed 53, 46, 71%. These results were significant different among the treatments(P<0.05). When the hCG were adminstrated at cloned embryo transfer, pregnanacy rate were control, hCG 5.8%, 10.4% respectively and there was no significant different between treatments. Plasma P4 concentration of hCG treatment in heifers were 3 times higher than control on 13~16 day after heat. After this, plasma P4 concentration of CIDR and hCG treated heifers were kept the 2~3 times levels. IGF-I concentration were showed no differences between pregnancy and non-pregnancy. hOG and CIDR. IGF-II concentration were revealed the differences between pregnancy and non-pregnancy in CIDR group but there was no differences in hCG administration group. Plasma cortisol were high at heat and 16 day after heat and CIDR treated group was higher than the other group. These results suggest that hCG administration was improve the pregnancy rates on Al and cloned ET, accompanying the incline of P4 concentration.

본 연구는 한우미경산우에 인공수정 7일째와 복제수정란 이식시 hCG를 투여에 의해 수태율 향상을 위하여 실시하였다 인공수정 후 임신 60일째에 임신감정을 실시한 결과 대조구, CIDR 그리고 hCG를 투여한 결과 53%, 46%, 71%였으며, 처리간에 유의적인 차이를 보였다(P<0.05). 복제수정 란의 이식시 hCG를 투여한 결과 대조구, hCG투여구는 각각 5.8%, 10.4%를 나타냈으며, 유의차는 인정되지 않았다. 인공수정 7일째에 hCG를 투여한 한우 미경산우의 발정일부터 발정 28일까지의 혈중 P4농도는 발정초기에는 처리구 모두 약간의 상승을 나타냈으며, 임신을 위한 가장 중요한 시기인 발정 13~16 일에는 무처리구에 비하여 hCG투여구가 3배 이상 의 수준을 나타냈으며, CIDR처리 임신우에 비교 하여도 약 B배 이상의 P4농도를 나타내었다. 그 이 후에도 P4의 농도는 2~3배의 수준을 지속적으로 유지하여 임신을 위하여 안정된 P4의 농도를 나타내었다. 인공수정 7일째 hCG를 투여한 한우 미경산우의 IGF-I 및 IGF-II의 혈중호르몬 농도는 IGF-I은 임신.비임신 그리고 hOG 및 CIDBt의 처리에 관계없이 큰 차이를 보이지 않았다. IGF-II는 CIDR(E2 capsule 제거) 처리구에서 임신이 비임신에 비하여 높은 경향을 나타냈으나, hCG 투여구에서는 임신과 비임신간에 큰 차이를 보이지 않았다. 인공수정 7일째에 hCG를 투여한 한우 미경산우의 발정일로부터 28일째까지의 혈중 cortisol 농도 는 발정당일과 발정 16일째의 임신한 미경산우에 서는 다른 발정주기일보다는 약간 높은 경향을 보였으며, 임신CIDR의 경우가 비임신보다는 전반적으로 높게 나타났고, hCG를 투여한 것은 큰 변화를 보이지 않았다. 이상의 결과에서 인공수정 및 복제수정란의 이식시 hCG 1,5001u를 투여한 한우 미경산우는 임신유지를 위한 혈중 P4의 농도를 상승시키며 수태율도 향상시키는 것으로 나타났다. IGF와 cortisol은 hCG 투여가 초기 임신기에는 큰 영향을 미치지 않는 것으로 나타났다.

Keywords

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