Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Immunohistochemical detection of infectious hematopoietic necrosis virus antigens in cell cultures

배양세포에서 전염성조혈장기괴사증 바이러스항원의 면역조직화학적 검출

  • 문운경 (국립수의과학검역원) ;
  • 이민권 (경상남도축산진흥연구소 북부지소) ;
  • 진영배 (경상대학교 수의과대학 동물의학연구소) ;
  • 김순복 (경상대학교 수의과대학 동물의학연구소)
  • Published : 2002.09.01
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Abstract

This experiment was done to set up the immunohistochemical detection method for infectious hematopoietic necrosis virus(IHNV) antigens in the monolayers of CHSE-214 cell cultures inoculated with IHNV. Specific identification of IHNV antigens was detected in the cytoplasms of infected cells by the use of monoclonal antibodies to glycoproteins. The specific positive signal was observed as a distinct red color. The result showed that streptavidin alkaline phosphatase immunohistochemistry specifically identified IHNV antigens in infected cultured cells.

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References

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