Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
권호 표지

Changes of Vaginal Epithelial Cells in Korea Jin-do Bitches after Induction of Estrus with PMSG and hOG

PMSG와 hCG 병용투여에 의해 인공발정 유기된 진도개에서 질상피세포 변화상

  • Published : 2002.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Estrus was induced in 13 anestrus Korea Jin-do bitches by intramuscular injection of pregnant mare serum gonadotropin (PMSG) in a dose of 500 lU once daily for ten consecutive days, followed by an additional single intraveneous injection of 1,000 lU of human chorionic gonadotropin (hCG) on the tenth day. Day-changes of vaginal epithelial cells during the hormone treatment were investigated in each experimental bitches and compared with the those of spontaneous estrus bitches. The first days of vulval bleeding and male acceptance after PMSG treatment were on Day 6.0$\pm$ 1.5 (mean$\pm$ SD) and Day 9.0$\pm$ 1.9, respectively. And in all of 13 bitches, vulval swelling and perineal reflex were shown. The mean durations of proestrus and estrus were 2.9$\pm$ 1.4 (mean$\pm$ SD, range ; 1-6) and 11.5: 1.7 (range ; 8-14) days, respectively, that is, duration of proestrus was significantly shorter than that of the spontneous estrous bitches but duration of estrus was longer than that of the spontaneous estrous bitches. Characteristic features of vaginal cytology during the estrous cycle were the high proportions of large intermediate cell, superficial cell, anuclear cell and erythrocyte in proestrus, superficial cell and anuclear cell in estrus and parabasal cell, small intermediate, large intermediate cell, and leukocyte in diestrus, respectively. The comification index (Cl) was significantly high proportion in proestrus and estrus, when Day 0 was timed from the first day of male acceptance, the Cl was first increased above 80% on Day 0 and maintained above 80% until Day 0 to Day 5 during 6 days and showed a peak on Day 2. Also it was maintained above 90% until Day 2 to Day 3 during 2 days. These results indicated that all 13 ekperimental bitches showed positive estrus detection by the estrus behavior and vaginal smear test after treated with PMSG and hCC. It suggested that vaginal cytology was used to estimate the optimal mating and ovulation time, in consideration of the day when the Cl was maintained above 80% in estrus-induced Korea Jin-do bitches.

Keywords

References

  1. Vet Med Small Animal Clinician A surgical method for collecting canine embryos aftr induction of estrus and ovulation with exogenous gonadotropins Archbald LF;Baker BA;Clooney LL;Godke RA
  2. J Reprod Fert (Suppl) v.39 Effect of duration of PMSG treatment on induction of oestrus, pregnancy rates and the complications of hyperoestrogenism in dogs Arnold S;Arnold P;Concannon PW;Weilenmann R;Hubler M;Casal M;Dobeli;Fairburn A;Eggenberger E;Rusch P
  3. Vet Rec v.88 Erythrocytes and leukocytes in the vaginal smears of the beagle bitch Bell ET;Christie DW
  4. Theriogenology v.35 Determination of ovulation time in bitches based on teasing, vaginal cytology, and ELISA for progesterone Bouchard GF;Solorzano N;Concannon PW;Youngquist RS;Bierscheal CJ
  5. Am J Vet Res v.49 Use of pulsatil intravenous administration of gonadotrophin releasing hormone to induce fertile estrus in bitches Cain JL;Cain GR;Feldmen EC;Lasley BL;Stabenfelt GH
  6. Br Vet J v.140 Induction of ovarian activity in anoestrous beagle bitches Chaffauzx S;Locci D;Pontois M;Deletang F;Thibier M.
  7. Reproduction in the dog and cat(1st ed) Breeding and mating Christiansen IJ
  8. Br Vet J v.128 Classification of cell types in vaginal smears during the canine oestrous cycle Christie DW;Bailey JB;Bell ET
  9. J Reprod Fert(Suppl) v.39 Induction of fertile oestrus in anoestrous dogs by constant infusion of GnRH agonist Concannon PW
  10. Biol Reprod v.17 progesterone and sexual behavior associatedl with preovulatory luteinization in the bitch Concannon PW;Hansel W;McEntee K;Change in LH
  11. Current Veterinary Therapy XI Methods for rapid induction of fertile estrus in dogs Concannon PW;Kirk RW;Bonagura JD
  12. Current veterinary therapy(10th ed) Hormonal and clinical correlates of ovarian cycles, ovulation, pseudopregnancy and pregnancy in dogs Concanon PW;Lein DH
  13. J Reprod Fert v.39 Biology and endocrinology of ovulation, pregnancy and parturition in the dog Concannon PW;McCann JP;Temple M
  14. Vet Rec v.129 ELISA determination of whole blood and plasma progesterone concentrations in the bitches Elgland GCW
  15. J Reprod Fert v.93 Repeatability of events during spontaneous and gonadotrophin induced oestrus in bitches England GCW;Allen WE
  16. Vet Rec v.125 A comparison of radioimmunoassay with quantitative and qualitative enzymelinked immunoassay for plasma progesterone detection in bitches England GCW;Allen WE
  17. Canine and feline endocrinology and reproduction(2st ed) Ovarian cycle and vaginal cytology Feldman EC;Nelson RW
  18. Canine and feline endocrinology and reproduction(2st ed) Canine female reproduction Feldman Ec;Nelson RW
  19. Veterinary Technician v.9 Canine vaginal cytology Guyant L
  20. J Reprod Fert (Suppl) v.47 Comparison of induction of oestrus in dogs using metergoline, metergoline plus human chorionic gonadotrophin, or pregnant mares serum gonadotrophin Kusuma PS;Tainturier D.
  21. Induction of estrus in bitches with exogenous gonadotropins, and pregnancy rate and blood progesterone profiles v.47 Nakao T;Aoto Y;Fukushima S;Moriyoshi M;Kawata K.
  22. Vet Quart v.13 Influence of litter size and breed on variation in length of gestation in the dog Qkkens AC;Hekerman TWN;De Vogel JWA;van Haaften B
  23. In vivo investigation of luteal function in dogs: effects of cabergoline, a dopamine agonist, and prolaction on progesterone secretion during mid-pregnancy and diestrus v.14 Onclin K;Verstegen JP
  24. J Am Vet Med Assoc v.171 Effects of exogenous follicle stimulating hormone and luteinizing hormone in bitches Paisley LG;Fahning ML
  25. Can Vet J v.26 Canine vaginal cytology during the estrous cycle Post K
  26. Can Vet J v.31 The evaluation of an enzyme immunoassay for the assesment of progesterone in canine plasma Post K
  27. J Small Anim Pract v.8 Canine vaginal cytology; Technique and cytological morphology Schutte AP
  28. J Small Anim Pract v.b no.8 Canine vaginal cytology; Cyclic changes Schutte AP
  29. J Small Anim Pract v.c no.8 Canine vaginal cytology; Compilation and evaluation of cellular indices Schutte AP
  30. Current therapy in theriogenology(ed.) Clinical reproductive physiology in dog Shille VM;Stabenfeldt GH
  31. JAVMA v.184 Efforts to induce estrus in the bitch, using pituitary goandotropins Shille VM;Thatcher MJ;Simmons KJ
  32. Am J Vet Res v.38 Induction of estrus and ovulation in the bitch using exogenous gonadotropins Thun R;Watson P;Jackson GL
  33. Jpn J Anim Reprod v.21 Studies on the physiology of reproduction in the dog; Observation of vaginal smear in estrous cycle Tsutsui T
  34. Theriogenology v.51 Effect of stage of anestrus on the induction of estrus by the dopamine agonist cabergoline in dogs Verstegen JP;Onclin K;Silva LD;Concannon PW
  35. Am J Vet Res v.53 Ultrasonographic appearance of the ovaries of dogs during the follicular and luteal phases of the estrous cycle Wallace SS;Mahaffer MB;Miller DM;Thompson FN;Chakraborty PK
  36. J Reprod Fert (Suppl) v.39 Ultrasonographic apprarance of the dog ovary during follicular and early Iuteal phases Wallace SS;Mahaffer MB;Miller DM;Thompson FN;Chakraborty PK
  37. Estrus induction in bitches by the administration of PMSG and HCG v.135 Weienmann R;Amold S;Dobeli M;Rusch P;Zerobin K
  38. J Small Anim Pract v.31 Application of vaginal cytology and plasma progesterone determinations to the management of reproduction in the bitch Wright PJ
  39. Aust Vet J v.56 The induction of oestrus and ovulation in the bitch using pregnant mare serum gonadotrophin and human chorionic gonadotrophia Wright PJ
  40. 대한수의학회지 v.28 인공적 발정유기견의 질세포상에 관한 연구 강병규;최한선;나진수;손창호;오기석;이차범
  41. 한국임상수의학회지 v.17 진돗개에서 발정주기 동안 질세포상과 번식호르몬의관계 김정훈;정경아;강현구;오기석;박인철;박상국;한호재;손창호
  42. 한국임상수의학회지 v.13 진도개에서 발정주기, 교배적기 및 배란시기 판정을 위한 질세포검사의 이용성 손창호;백인석;신창록;최한선;강병규
  43. 대한수의학회지 v.36 진도개에서 임신일령에 따른 임신구조물의 초음파상; 태아 및 태아외구조물의 최초 관찰시기 손창호;신창록;강병규;최한선
  44. 한국임상수의학회지 v.13 인공발정유도견에서 질상피세포의 변화 유일정;김용준;지동범