Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

Effect of Conjugated Linoleic Acid on Colon Tumor Incidence and Antioxidant Enzymes and fecal Excretion of Secondary Bile Acids in DMH-treated Rats

쥐에서 Conjugated Linoleic Acid가 대장의 종양발생률과 항산화효소와 Eicosanoid 및 2차 담즙산 배설에 미치는 영향

  • 김경희 (전남대학교 의과학연구소) ;
  • 강금지 (덕성여자대학교 식품영양학과) ;
  • 박현서 (경희대학교 식품영양학과)
  • Published : 2002.12.01
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Abstract

The study was designed to observe the effect of conjugated linoleic acid (CLA) on tumor incidence, eicosanoid formation and antioxidant enzyme activities in colonic mucosa and the fecal excretion of deoxycholic acid and lithocholic acid in 1,2-dimethylhydrazine (DMH)-treated rats. One hundred twenty male Sprague Dawley rats were divided into 2 groups, BT (beef tallow diet) group and FO (fish oil diet) group, and each group was again subdivided into 2 groups depending on CLA supplementation, i.e.4 groups of BT, BTC, FO, FOC. All rats were fed experimental diet for 30 weeks, which contained 12% (wt/wt) total dietary fat including 1% (wt/wt) CLA, and were intramuscularly injected with DMH for 6 weeks to give total dose of 180 mg/kg body. CLA-supplemented to BT and FO diet reduced tumor incidence, eicosanoid (PGE$_2$ and TXA$_2$) level in colonic mucosa. N-3 fatty acids (mainly DHA) of fish oil diet (FO, FOC group) also reduced tumor incidence and significantly reduced eicosanoid (PGE$_2$ and TXA$_2$) level in colonic mucosa. CLA supplementation and n-3 fatty acid significantly increased colonic mucosal level of superoxide dismutase and glutathione peroxidase activities but reduced secondary bile acids (deoxycholic acid and lithocholic acid) excretion in the feces. In conclusion, CLA supplementation and n-3 fatty acid could reduce tumor incidence by reducing eicosanoids and increasing antioxidant enzyme activities in colon and decreasing the excretion of deoxycholic acid and lithocholic acid in the feces. The data might suggest that CLA supplementation and n-3 DHA rich fish oil may modulate colon carcinogenesis.termediate level of endurance exercise training for 6 weeks did not influence concentrations of most of free amino acid in soleus muscle of rats collected at an overnight fasted and rested state. In contrast, isolucine and leucine concentrations in extensor digitorum longus muscle of exercise-trained rats were significantly lower than those for control animals. These results indicate that aerobic energy metabolism had not been efficiently conducted, and thereby the utilization of BCAA for energy substrate was enhanced in fast twitch oxidative glycolytic fibers of extensor digitorum longus muscle of rats followed exercise-training protocol for 6 weeks.

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References

  1. Carcinogenesis v.8 Anticarcinogens from fried ground beef: heat-altered derivatives of linoleric acid Ha YL;Grimm NK;Pariza MW https://doi.org/10.1093/carcin/8.12.1881
  2. Cancer Res v.50 Inhibition of benzo(a)pyrene induced mouse forestomach neoplasia by conjugated dienoic derivatives of linoleic acid Ha YL;Strokson JM;Pariza MW
  3. Cancer Res v.45 Carcinogen-induced lesions in rat Pancreas: effect of varying levels of essential fatty acid Roebuck BD;Longnecker DS;Baumgartner KJ;Thron CD
  4. Cancer Res v.45 Requirement of essential fatty acid for mammary tumorigenesis in the rat Ip C;Carter CA;Ip MM
  5. Cancer Res v.54 Conjugated linoleic acid suppresses mammary gland in rat Ip C;singh M;Thompson HJ;Scimeca JA
  6. Poultry Sci v.72 Immune modulation by altered nutrient metabolism: Nutritional control of immune-induced growth depression Cook ME;Miller CC;Park Y;Pariza MW https://doi.org/10.3382/ps.0721301
  7. Korean J Nutrition v.34 no.4 Effects of conjugated linoleic acid supplementation on fat accumulation and degradation in rats Kang KJ;Park HS
  8. Korean J Nutrition v.9 no.4 Effect of dietary conjugated linoleic acid on plasma lipid composition in rats Jung SM;Kang KJ;Park HS
  9. Korean J Nutrition v.33 Mechanism of cancer prevention and other physiological function by conjugated linoleic acid Park HS
  10. Cancer Letters v.150 Reduction of murine mammary tumor metastasis by conjugated linoleic acid Hubbard NE;Lim D;Summers L;Erickson KL https://doi.org/10.1016/S0304-3835(99)00379-1
  11. Nutr Cancer v.26 Inhibition of mouse skin tumor promotion by dietary conjugated linoleate Belury MA;Bird C;Nickel KP;Wu B https://doi.org/10.1080/01635589609514471
  12. Anticancer Res v.18 Opposite effect of linoleic acid and conjugated linoleic acid on human prostatic cancer in SCID mice Cesano A;Visonneau S;Scimeca JA;Kritchevsky D;Santoli D
  13. Carcinogenesis v.16 Protection of conjugated linoleic acids against 2-amino-3-methylimidazo[4,5-f]quinolin-induced colon carcinogenesis in the F344 rat: a study of inhibitory mechanisms Liew C;Shut HA;Chin SF;Pariza MW;Dashwood RH https://doi.org/10.1093/carcin/16.12.3037
  14. Advances in cancer research v.53 Anti-oxidants: carcinogenic and chemopreventive properties Ito N;Hirose M;Vande Woude GF(ed.);Klein G(ed.) https://doi.org/10.1016/S0065-230X(08)60283-3
  15. Cancer Res v.51 Mammary cancer prevention by conjugated dienoic derivative of linoleic acid Ip C;Chin SF;Scimeca JA;Pariza MW
  16. Br J Nutr v.86 Dietary conjugated linoleic acid induces apoptosis of colonic mucosa in 1,2-dimethylhydrazine-treated rats: a possible mechanism of the anticarcinogenic effect by CLA Park HS;Ryu JH;Ha YL;Y. Park JH https://doi.org/10.1079/BJN2001445
  17. J Lab Clin Med v.85 no.2 The estimation of red cell superoxide dismutase activity Winterbourn CC;Hawkins RE;Brian M;Carrell RW
  18. J Lab Clin Med v.70 no.1 Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase Paglia DE;Valentine WN
  19. Adv Prostaglandin Thromboxane Res v.5 Quantitative measurement of prostaglandins and thromboxanes: general consideration Granstrom E;Samuelesson B
  20. Methods Biochem Anal v.17 Separation, identification and estimation of prostaglandins Shaw JE;Ramwell PW https://doi.org/10.1002/9780470110355.ch8
  21. Japan J Pharmacol v.27 Effect of dietary cholesterol on cholesterol and bile acid metabolism in rats Uchida K;Nomura Y;Kadiwaki M;Takeuchi N;Yamamura Y https://doi.org/10.1254/jjp.27.193
  22. Cancer Res v.51 Mammary cancer prevention by conjugated dienoic derivative of linoleic acid Ip C;Chin SF;Scimeca JA;Pariza MW
  23. Cancer Letters v.177 The antiproliferative effects of biologically active isomers of conjugated linoleic acid on human colorectal and prostatic cancer cells Palombo JD;Ganguly A;Bistrian BR;Menard MP https://doi.org/10.1016/S0304-3835(01)00796-0
  24. Pharmacology & Therapeutics v.83 Omega-3 fatty acids as cancer chemopreventive agents Rose DP;Connolly JM https://doi.org/10.1016/S0163-7258(99)00026-1
  25. Cancer Res v.51 Mammary cancer prevention by conjugated dienoic derivative of linoleic acid Ip C;Chin SF;Scimeca JA;Pariza MW
  26. Master Thesis, Kyung Hee University Effect of conjugated linoleic acid on hepatic peroxidation status and antioxidant enzyme system in rats Yoon KM
  27. J Korean Soc Food Nutr v.24 no.4 Naturally-occurring novel anticarcinogens: conjugated dienoic derivatives of linoleic acid (CLA) Ha YL;Pariza MW
  28. Lipids v.30 no.7 Reinvestigation of the antioxidant properites of conjugated linoleic acid Jeroen JM van den Berg;Cook NE;Tribble DL https://doi.org/10.1007/BF02536996
  29. Cancer Epidemiol Biomarkers & Prev v.9 Novel approaches to the prevention of colon cancer by nutritional manipulation and chemoprevention Reddy BS
  30. Biochim Biophys Acta v.1258 Selected eicosanoids increase the proliferation rate of human colon carcinoma cell lines and mouse colonocytes in vivo Qiao L;Kozoni V;Tsioulias GJ;Koutsos MI;Hanif R;Shiff SJ;Rigas B https://doi.org/10.1016/0005-2760(95)00100-Q
  31. Cancer Res v.49 Differential proliferative response to linoleate in cultures of epithelial cells from normal human breast and fibroadenomas Balakrishnan A;Cramer S;Banyopadhyay GK;Imagawa W;Yang J;Elias J;Beattie CW;Gupta TDK;Nandi S
  32. Cancer Res v.50 Effect of fatty acid and inhibitors of eicosanoid synthesis on the growth of a human breast cancer cell line in culture Rose DP;Connally JM
  33. Prostaglandins Leukot Essent Fatty Acids v.45 Regulation of proliferation of rat mammary tumor cells by inhibitiors of cyclooxygenase and lipoxygenase Lee PP;Ip MM https://doi.org/10.1016/0952-3278(92)90098-4
  34. Exp Hematol v.17 Antiproliferative effects of lipoxygenase inhibitors on malignant human hematopoietic cell lines Snyder DS;Castro R;Desforges JF
  35. Biochim Biophys Acta v.1438 Antiplatelet effects of conjugated linoleic acid isomers Truitt A;Mcneill G;Vanderhoe JY https://doi.org/10.1016/S1388-1981(99)00055-4
  36. Cancer Letters v.127 Conjugated linoleic acid reduces arachidonic acid content and PGE2 synthesis in murine keratinocytes Liu K;Belury MA https://doi.org/10.1016/S0304-3835(97)00479-5
  37. Lipids v.32 Conjugated linoleic acid modulates hepatic lipid composition in mice Belury MA;Kempa-Steczko A https://doi.org/10.1007/s11745-997-0025-0
  38. Biochim Biophys Acta v.210 Inhibition of prostaglandin biosynthesis by 8cis, 12tans,14cis-eicosatrienoic acid and 5cis, 8trans,12trans,14cis-eicosatetraenoic acid Nugteren DH https://doi.org/10.1016/0005-2760(70)90072-X
  39. Colorectar Cancer Arachidonic acid metabolism and colorectal cancer DeRubertis FR;Craven PA
  40. Eur J Cancer v.31 Role of bile acids in colorectal carcinogenesis Nagengast FM;Grubben MJAL;vanMunster IP https://doi.org/10.1016/0959-8049(95)00216-6
  41. Cancer Res v.41 Dietary fat and its relationship to large to large bowel cancer Reddy BS
  42. J Clin Inves Inc v.74 Bile salt stimulation of colonic epithelial proliferation Derubertis FR;Craven PA;Saito R https://doi.org/10.1172/JCI111577
  43. Colorectar Cancer The role of bile acids in colorectal carcinogenesis Cohen BI;Deschner EE
  44. Cancer Res v.46 no.11 Relationship between loss of rat colonic surface epithelium induced by deoxycholate and initiation of subsequent proliferative response Craven PA;Pfanstiel J;Saito R;DeRubertis FR