생명과학회지 (Journal of Life Science)

  • 제12권6호
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  • Pages.759-764
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  • 2002
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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해양미생물로부터 면역증강물질의 생산 최적화

Optimization of the Production of an Immunostimulant from a Marine Bacterium

  • 발행 : 2002.12.01
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초록

해수에서 분리한 Burkholderia sp. IS-203으로부터 면역 증강물질을 생산하기 위한 최적조건을 검토한 결과는 다음과 같다. 면역증강물질을 생산하는 최적 배양조건은 인공해수에 1% glucose와 1% yeast extract 였으며, 최적 NaCl 요구성은 3%로 전형적인 해양세균의 특징을 보여주는 것으로 생각되어진다. 그리고 최적 배양조건으로 최적 초기 pH는 8.0, 최적 온도는 $30^{\circ}C$였으며, 산소를 요구하는 호기성균이었다. 면역증강물질을 생산하기 위한 배양조건은$35^{\circ}C$, pH 6.0이었으며, 최적생육 배지는 1.5% soluble starch, 1% tryptone, 0.5% yeast extract로 조성하여 500 $m\ell$의 삼각플라스크에 $50m\ell$씩 분주하여 진탕배양하였다. 이와 같은 최적조건에서 항생물질은 정지기부터 생산되는 비증식연관형으로서 포자형성이후부터 생산되었다.

A halophilic bacterium for the production of the immunostimulant was isolated from domestic marine, it was identified as Burkholderia sp. IS-203. The optimal conditions for the production of the immunostimulant were 1 % dextrose and 1 % yeast extract in artificial sea water for carbon and nitrogen sources, respectively. The initial pH and growth temperature for the prodution were 8.0 and $30^{\circ}C$ under the presence of oxygen, respectively.

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참고문헌

  1. J. Biol. Chem. v.261 Modulation of receptor and cytotoxic response of tumor necrosis factor-L by various lectins Agarwal, B. B.;P. R. Traquna;T. E. Eessalu
  2. J. Korean Soc. Food Sci. Nutr. v.29 Isolation and Identification of a Marine Bacterium Production an Immunostimulant Choi, H. J.;M. J. Jung;Y. K. Jeong
  3. Cancer Res. v.49 Biological response modifiers: The new immunotherapy Food, K. A.
  4. Trends Biochem. Sci. v.14 Characterization of oligosaccharides by lectin affinity highperformance liquid chromatography Green, E. D.;J. U. Baenziiger https://doi.org/10.1016/0968-0004(89)90267-3
  5. N. Y. Acad. Sci. v.284 Levanisole and inosiplex: Antivial agent with immunopotentiating action Hadden, J. W.;C. Lopez;R. I. O'Rerlly;E. M. Hadden https://doi.org/10.1111/j.1749-6632.1977.tb21945.x
  6. Int. J. Immunopharmac v.1 Immunopharmacology of mice and men Hadden, J. W. https://doi.org/10.1016/0192-0561(79)90024-9
  7. Chem. Pharm. Bull. v.39 Mitogenic and Colony-stimulating factor inducing activity of polysaccharide fractions from the fruit bodies of Dictyohora indusiata Fisc Hara, C.;Y. Kumazawa;K. Inagki;M. Kaneko;T. Kiho;S. Ukai https://doi.org/10.1248/cpb.39.1615
  8. Comp. Biochem. Physiol. v.88B N-acethylgalactosamine-specific lectin, a novel lectin in the hemolymph of the ascidian Halocynthia roretzi: Isolation, characterization and comparison with galactose-specific lectin Harada, A. K.;H. Yokosawa;S. I. Ishii
  9. J. Cancer Res. v.76 Antitumor effects of Sarcophage lectine on murine trans planted tumors Itoh, A.;K. Iizuka;S. Natori
  10. Gann. v.72 Antitumor activity of Lactobacillus casei in mice Kato, I.;S. Kobayashi;T. Yokokura;M. Mutai
  11. Immunol. v.47 Clinical and immunologican separated from hot water extract of Angelica acutiloba Kitagawa (Yamato Tohki) Kumazwa, T.;J. Imai;S. Tamakuma
  12. Cancer Res. v.45 Successful immunotherapy of murine experimental hepatic metastases with lymphokine-activated killer cells and recombinant interleukin-2 Lafreniere, R.;S. A. Rosenberg
  13. J. Immunol. method v.65 MTT assay Mosmann, T. https://doi.org/10.1016/0022-1759(83)90303-4
  14. J. Natl. Cancer Inst. v.70 Biological response modifiers Oldham, R. K.
  15. Immunology v.75 Enhancement of the neonate's nonspecific immunity to Klebsiella infection by muramyl dipeptide, a synthetic immunoadjuvant Parent, M.;F. Parent;L. Chedid
  16. Handbook of Experimental Immunology v.2 Severinson E.;E.L. Larsson;Weir D. M.(ed.)
  17. Isr. J. Med. Sci. v.12 Cellular immune competence in patient with tumors of the gastrontestinal tract Shohat, B.;H. Joshna;I. Kott;I. Irea
  18. Cancer Res. v.49 A fluorine-containing anthracycline (ME 2303) as a new antitumor agent againt murine and human tumors and their multidrug resistant sublines Tsuru, T.;K. Yusa;Y. Sudo;R. Takamori;Y. Sugimoto
  19. Current Topics in Marine Biotechnology Watanabe, I.
  20. J. Immunol. v.121 Inosiplex: A stimulating agent for normal human T cell and human leucocytes Wybran, J.;A. Govaerts;T. Appelboom
  21. Comp. Immunol. v.5 Invertebrate lectin: Ⅰ. Occurrence. Dev. Yeaton, R. W. https://doi.org/10.1016/S0145-305X(81)80052-3
  22. Planta Medica. v.63 Fractionation and dhemical properties of immunomodulating polysaccharides from roots of Dipsacus asperoides Yongwen Z.;K. Hiroaki;M. Tsukasa;Y. Haruki https://doi.org/10.1055/s-2006-957720