Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Characteristics of Biosurfactants produced by Bacillus sp. LSC11

Bacillus sp. LSC11가 생산하는 biosurfactant의 특성

  • 이상철 (동아대학교 생명자원과학부) ;
  • 정연주 (동아대학교 생명자원과학부) ;
  • 유주순 (동아대학교 생명자원과학부) ;
  • 조영수 (동아대학교 생명자원과학부) ;
  • 차인호 (부산시 보건환경연구원) ;
  • 최용락 (동아대학교 생명자원과학부)
  • Published : 2002.12.01
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Abstract

Several bacterial strains producing biosurfactants were isolated from polluted marine and soil by oil. One of the strains named LSC11 showed strong production activity of biosurfactants. This strain was identified as a Bacillus sp. LSC11 based on the morphological, biochemical, and physiological characteristics. The biosurfactant, produced by the strain, emulsified crude oil, vegetable oil, and hydrocarbons. The surface tension of the culture broth of Bacillus sp. LSC11 decreased to 32 mN/m. The crude biosurfactant was obtained from the culture broth by acid precipitation, freeze drying, solvent extraction, and evaporation. The emulsifying activity of the biosurfactant showed better than the chemically synthesized surfactant (SDS, Span40, Span 85). The biosurfactants had strong properties as an emulsifying agent and as an emulsion-stabilizing agent.

원유 분해능이 강력한 해양균주를 얻고자 유류오염 지역으로부터 crude oil을 탄소원으로 이용하는 수십 종을 분리하였다. 분리된 균주중 원유분해능 및 biosurfactant 생성능이 우수한 균주를 선별하여, 형태학적, 생화학적 및 생리학적 특성을 조사한 후 Bacizzus sp. LSC11로 동정하였다. Bacillus sp. LSC11 균주 배양액의 표면장력은 최저 32mN/m까지 감소되었다. Biosurfactant의 CMC값은 0.0035% (w/v) 인 것으로 나타났다. Bacillus sp. LSC11가 생산하는 biosurfactant의 유화활성은 대두유에서 최대였으며, 원유에서도 높은 편이였다. 유화안정성은 합성계면활성제와 비슷하거나 우수하였다.

Keywords

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