생명과학회지 (Journal of Life Science)

  • 제12권6호
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  • Pages.740-744
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  • 2002
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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몇 가지 나리 품종의 기내배양에서 배지의 물리성이 자구증식 및 순화에 미치는 영향

Effects of Physiological Properties in Culture media on the Proliferation and the Acclimation of Bulblets of Lilies Cultured in vitro

  • 발행 : 2002.12.01
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초록

배지의 물리성이 몇 가지 나리 품종 자구의 기내 증식과 증식된 자구의 순화에 미치는 영향을 구명하기 위해 수행되었다. 기내 인편배양과 자구배양에 의한 자구의 증식은 Oriental hybrid ‘Marco Polo’, ‘Casa Blanca’와 Asiatic hybrid ‘Jolanda’ 세 품종 모두 액체배지보다 고체배지에서 자구로부터 자구를 증식시켰을 때 형성된 자구의 수와 생장이 가장 양호하였다. 기외 순화단계에서는 모든 실험구에서 전배양이 고체배지였던 실험구가 생존율과 맹아율이 가장 높았으며, 특히 전배양에서 자구를 실험재료로 이용했던 실험구가 인편을 이용하였던 것보다 맹아율이 다소 양호하였다.

This experiment was conducted to clarify the effects of physiological properties in culture media on the proliferation and the acclimation of bulblets of several kinds of lilies cultured in vitro. Proliferation rate of bulblets from bulblet of Oriental hybrid ‘Marco Polo’, ‘Casa Blanca’ and Asiatic hybrid ‘Jolanda’ was higher in solid MS media compared with bulblets from scale Acclimation rate of regenerated bulblets was high in solid media which was pre-cultured in vitro, compared with liquid media. Sprouting rate of bulblets from bulblet was higher than bulblets from scale.

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참고문헌

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