Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Characterization of the enzymatic property of thermostable carboxypeptidase Taq by addition of metal ions and replacement of active center metal

금속이온 첨가와 활성중심 금속의 치환에 따른 내열성 카르복시펩 티다제 Taq의 효소적 특성 변화에 관한 연구

  • Lee, Sang-Hyeon (Department of Bioscience and Biotechnology, Silla University) ;
  • Ha, Jong-Myung (Department of Bioscience and Biotechnology, Silla University) ;
  • Ha, Bae-Jin (Department of Bioscience and Biotechnology, Silla University)
  • Published : 2002.12.01
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Abstract

We analyzed improvement on the enzyme activity of CPase Taq by addition of various metal ions. The enzyme activity was increased more then four times by 1 mM cobalt ion and almost three times by 1 mM calcium ion. However, the active center metal zinc ion did not affect the enzyme activity. In order to investigate whether the active center metal affects the enzyme activity, zinc ion which is occupied the active center of the enzyme was replaced by cobalt ion which activates the enzyme activity very effectively. Since the cobalt ion in the active center of the cobalt-substituted CPase Taq did not affect the enzyme activity, it could act as the natal metal ion in the active center of the enzyme.

다양한 금속이온의 첨가에 따른 CPase Taq의 효소활성의 개선에 관한 연구를 행하였다. 1 mA의 코발트이온의 첨가에 의해 효소활성이 4배 이상 증가했고, 1 mA의 칼슘이온의 첨가에 의해서는 효소활성이 거의 3배 정도로 증가했다. 하지만 활성중심에 존재하는 아연이온은 효소활성에 영향을 주지 않았다. 활성중심의 금속 이온이 효소활성에 영향을 주는지를 알아보기 위해 활성중심을 차지하고 있는 아연이온을 본 효소를 효과적으로 활성화시키는 코발트이온으로 치환하였다. 그 결과, 코발트이온으로의 치환이 CPase Taq의 효소활성에 영향을 주지 않으므로, 코발트이온은 본 효소의 활성중심의 금속이온인 아연이온을 대신하여 CPase Taq가 효소활성을 가지는데 있어서 동일한 역할을 할 수 있는 금속이온이라 사료된다.

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References

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