Monitoring Expression of bphC Gene from Ralstonia eutropha H85O Induced by Plant Terpenes in Soil

  • Jung, Kyung-Ja (Division of Civil Environmental Systems Engineering, Korea Maritime University) ;
  • Kim, Byung-Hyuk (Division of Civil Environmental Systems Engineering, Korea Maritime University) ;
  • Kim, Eungbin (Department of Biology, Yonsei University) ;
  • So, Jae-Seong (Department of Biological Engineering and Center for Advanced Bioseparation Technology, Inha University) ;
  • Koh, Sung-Cheol (Division of Civil Environmental Systems Engineering, Korea Maritime University)
  • 발행 : 2002.12.01

초록

A PCB degrader, Ralstonia eutropha H850 was shown to induce bphC gene encoding 2,3-dihydroxy-biphenyl-1,2-dioxygenase in a carvone-amended pure culture in our previous study (Park et al.,1999). The present study was carried out to examine how plant terpenes, as natural substrates, would cause an expression of a PCB degradative gene in soil that was amended with terpenes. The population of Ralstonia eutropha H850 was maintained at least around 10$\^$8/ (CFU/g fresh soil) in the soil amended with carvone or limonene in the presence of succinate as a growth substrate at 50 th day. The gene expression was monitored by RT-PCR using total RNA directly extracted from each soil and bphC gene primers. The bphC gene expression of the seeded strain H850 was observed in the soil amended with biphenyl (4 days) but not with succinate, carvone and limonene. These results indicate that terpenes widely distributed in nature could be a potential inducing substrate for effective PCB biodegration in the soil but their bioavailability and specific induction behavior should be taken into account before PCB bioremediation implementation.

키워드

참고문헌

  1. Crit. Rev. Biotechnol. v.10 Aerobic and anaerobic biodehradation of PCBs: a review. Abramowicz,D.A.
  2. Current Protocols in Molecular Biology. Ausubel,F.M.
  3. Appl. Environ. Microbiol. v.64 Brominated biphenyls prime extensive microbial reductive dehalogenation of Aroclor 1260 in Housatonic River sediment. Bedard,D.L.;H.Van Dort;K.A.Deweerd.
  4. Bioldegradation. v.3 Bacterial PCB Bioldegradation. Boyle,A.W.;C.J.Silvin;J.P.Hassett;J.P.Nakas;S.W.Tanenbaum.
  5. Fenaroli’s Handbook of Flavour Ingredients, 3rd ed. v.107 Burdock,G.A.
  6. Appl. Environ.Microbiol. v.63 Plant compounds that induce polychlorinated biphenyl biodehradation by Arthrobacter sp. strain BIB. Gilbert,E.S.;D.E.Crowley.
  7. Appl. Environ.Microbiol. v.50 Repeated application of Carvone-induced bacteria to enhance biodegradetion of polychlorinated biphenyls in soli. Gilbert,E.S.;D.E.Crowley.
  8. Biodegradation. v.8 Terpeneutilizing isolates and their relevance to enhanced biotranformation of PCBs in soil. Hernandez,B.S.;S.Koh;M.Chial;D.D.Focht.
  9. Environ.Pollut. v.92 Polychlorinated biphenyls in small mammals from contaminated landfill sites. Johnson,M.S.;R.T.Leah;L.Connor;C.Rae'S.Saunders.
  10. Biotechnol. Bioprocess Eng. v.6 Specific Biodegradation of Polychlorinated Biphenyls (PCBs) Facilitated by Plant Terpenoids. Jung,K.J.;E.Kim;J.S.So;S.C.Koh.
  11. J. Microbiol. v.39 Effect of moisture content in reductive dechlorination of polychorinated biphenyls and population dynamics of dechlorinating microorganisms. Kwon,O.S.;Y.E.Kim;J.G.Park.
  12. Appl. Environ.Microbiol. v.64 Construction of a bioluminescent reporter strain to dedect poly-chlorinated biphenyls. Layton,A.C.;M.Muccini;M.M.Ghosh;G.S.Sayler.
  13. Appl. Environ.Microbiol. v.60 Construction of a novel polychlorinated biphenyl-degrading bacterium: utilization of 3,4-dichlorobiphenyl by Pseudomonas acidovorams M3GY. McCullar,M.V.;V.Brenner;R.H.Adams;D.D.Focht.
  14. In Techniques in mictobiol ecology. Isolartion of nucleic acids from environmental samples. Ogram,A.
  15. J. Microbiol. v.38 Construction fo a cioluminescent reporter using the luc gene and meta-cleav-age dioxygenase promoter for detection of catecholic compounds. Park,S.H.;D.H.Lee;K.H.Oh;C.K.Kim.
  16. J.Microbiol. Biotechnol. v.9 Induction by carvone of polychlorinated biplenyl(PCB)-degradative pathway in Alcaligenes eutroplus H850 and its molecular monitoring. Park,Y.I.;J.S.So;S.C.Koh.
  17. Gene v.125 Cloning and expression of the polychorinated biphenyl-defradation gene cluster from Arthrobacter M5 and comparison to analogones from Gram-negative bacteria. P’eloquin,L.;C.W.Greer.
  18. Appl. Environ.Microbiol. v.54 Biodegradation of polychlorinated biphenyl-contaminated soil using carvone and surfactant-grown bacteris. Singer,A.C.;E.S.Gilbert;E.Luepromchai.D.E.Crowley.
  19. Appl. Environ.Microbiol. v.65 Rhodcoccus erythropolis DCL14 contain a nivel degradation pathway for limonene. Van der Werf,M.J.;J.A.M. de Bont.