Inferring the Molecular Phylogeny of Chroococcalian Strains (Blue-green algae/Cyanophyta) from the Geumgang River, Based on Partial Sequences of 16S rRNA Gene

  • Lee, Wook-Jae (Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology) ;
  • Bae, Kyung-Sook (Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology)
  • 발행 : 2002.12.01

초록

Partial sequences of 16S rRNA gene of five chroococcalian blue-green algal strains, Aphanothece nidulans KCTC AG10041, Aphanothece naegelii KCTC AG10042, Microcystis aeruginosa KCTC AG10159, Microcystis ichthyoblabe KCTC AG10160, and Microcystis viridis KCTC AG10198, which were isolated from water from the Geumgang River, were determined and were inferred their phylogenetic and taxonomic positions among taxa of order Chroococcales. Most taxa of Chroococcales whose partial 16S rRNA gene sequences were aligned in this study, are clustered with other related taxa. Aphanothece nidulans KCTC AG10041 and Aphanothece naegelii KCTC AG10042 made a cluster with other European species of these genera, which supported 100% of the bootstrap trees with a very high sequence similarity (97.4-99.4%) in this study. Three strains, Microcystis aeruginosa KCTC AG10159, M. ichthyoblabe KCTC AG10160, and M. viridis KCTC AG10198, formed a cluster with other Microcystis spp. supported 100 % of the bootstrap trees with a similarity of 97.0-99.9% except for two strains. However, this phylogentic tree made no resolution among the species of Microcystis spp. The topology of the tree reconfirmed the taxonomic status of three species of Microcystis, identified in this study based on the morphology, as three colonial types of Microcystis aeruginosa com. nov. Otsuka et al. (1999c). The genera of chroococcalian cyanophytes are heterogeneously clustered in these sequence analyses. We suggest that more molecular studies on the genera of Chroococcales with reference strains, widely collected from restricted geographic or environmental ranges, get accurate taxonomic or phylogenetic determinations.

키워드

참고문헌

  1. J. Phycol. v.37 Genetic variability of Brazilian strains of the Mictocystis aeruginosa complex(Cyanobacteria/Cyanophyceae) using the phycocyanin intergenic spacer and flanking regions (cpcBA). Bittencout-Olverira,M.C.;C.C. de Oliveira;C.J.S.Bolch.
  2. Bergeys manual of Systematic Bacteriology v.3 Taxa of the Cyanobacteria. Castenholz,R.E.;J.B.Waterbury;Staley,J.T.;M.P.Bryant;N.Pfeng;J.G.Holt(ed)
  3. PH. D. Thesis Computer-assisted classification and Identification of actinomycetes. Chun,J.
  4. Evolution v.39 Confidence limits on phylogenies: An approach using the bootstrap. Felsenstein
  5. PHYLIP(Phylogenetic inference package) version 3.5c. Felsenstein,J.
  6. Arch. Microbiol. v.169 Ulrich, The phylogeny of unicellular, extremely halotolerant cyanobatceria. Gracia-Pichel,F.;U.Nubel;G.Muyzer.
  7. J. Gen.Appl. Microbiol. v.43 Phylogenetic relationships of filamentous cyanobacterial taxa inferred from 16S rRNA sequence divergence. Ishida,T.;A.Yokota;J.Sugiyama.
  8. J. Mol.Evol. v.16 A simple method for estimating evolitionary rates of base substitutions through comparative studies of nucleotide sequences. Kimura,M.
  9. Arch. Hydrobiol. Suppl.Algol.Stud. v.64 Allozyme divergence in Mirocystis(Cyanophyceae) and its taxonomic inference. Kato,T.;M.F.Watanabe;M.Watanabe.
  10. Taxon v.6 Das " Microcystis"- problem. Kormarek,J.
  11. Arch. Hydrobiol. Suppl.Algol.Stud. v.64 A review of water-bloom forming Microcysis species, with regard to populations from Kapen. Kormarek,J.
  12. Arch. Hydrobiol. Suppl.Algol.Stud. v.43 Mordern approach to the classification system of Cyanophtes, 2Chroococcalce. Komarek,J.;K.Anagnostidis.
  13. Hydrobiologia v.308 Fatty acid composition as a taxonomic characteristic for Microcystis and cther coccoid cyanobacteria(blue-green alga) isolates. Kurger,G.H.;H.De Wet;J.L.F.Kock;A.J.H.Pieterse.
  14. Angae v.12 Taxonomic implications of the genes Microcystis (cyanophyta) from the Naktong River. Lee,J.A.;A.R.Choi;M.Watanabe.
  15. Korean. J. Limnol. v.32 Dynamics fo phytoplankton community in Lake Daechung. Lee,J.H.
  16. J. Microbiol. Biotechnol. v.11 The phylogenetic relationships of several oscillatorian cyanobacteria, forming blooms at Daecheong Reservoirs, based on partial 16S rRNA gene sequences. Lee,W.J.;K.S.Bae.
  17. Int. J. Syst.Bacteriol. v.47 rRNA sequences and evolutionary relationships among toxic and nontoxic cyanobacteria of the genus Microcustis. Neilan,B.A.;D.Jacobs,T.De Dot;L.L.Blackall;P.R.Hawkins;P.T.Cox;A.E.Goodman.
  18. Syst.Appl. Microbiol. v.17 Phylogenetic relationships among filamentous helical cyanobacteria investigated in the basis of 16S ribosomal rRNA gene sequence analysis. Nelissen,B.A.;Willmontte;J.-M.Neefs;R.DeWachter.
  19. J. Mol. Evol. v.42 Phylogenetic relationships of nonaxenic filamentous cyanobacteria strains based on 16S rRNA gene sequence analysis. Nelissen,B.;R.De Baere;A.Willmontte;R.De Wachter.
  20. Appl. Environ.Microbiol. v.63 PCR primers to amplify 16S rRNA genes from cyanobacteria. Nubel,U.;F.Garcia-Pichel;G.Muyzer.
  21. FEMS Microbiol,Lett. v.164 16S rDNA sequences and phylogenetic analyses of Microcystis strains with and without phycoerythrin. Otuska,S.;S.Suda;R.Li'M.Watanabe;H.Oyaizu;S.Masumoto;M.M.Watanabe.
  22. FEMS Microbiol. Lett. v.172 Phylogenetic relationships between toxic strains of the genus Microcystis based on 16S to 23S internal transcribed spacer sequence. Otucka,S.;S.Suda;R.Li;M.Watanabe;H.Oyaizu;S.Masumoto;M.M.Watanabe.
  23. Phycol. Res. v.47 Characterization of morpho-spe-cies and strains fo the genes Microcystis(cyanobacteria) for reconsideration of species classification. Otucka,S.;S.Suda;R.Li;M.Watanabe;H.Oyaizu;S.Masumoto;M.M.Watanabe.
  24. Int. J. of Syst. and Microbiol. v.51 A proposal for the unification of five species fo the cyanobacterial geeus Microcustis Kutzing ex Lemmermann 1907 under the rules of the bacterial code. Otucka,S.;S.Suda;R.Li;M.Watanabe;H.Oyaizu;S.Masumoto;M.M.Watanabe.
  25. ACBIOS v.12 TREEVIEW: An application to display phylogenetic trees on personal computers. Page,R.D.M.
  26. J. Microbiol. Biotechnol. v.10 Services of algae to the environment. Rai,L.C.;H.D.Kumar;F.H.Mohn;C.J.Soeder.
  27. PAUP. Phylogenetic analysis using parsimony. Version 4. Swofford,D.L.
  28. J. Microbiol. Biotechnol. v.9 Cyanobacterial toxins: The current status. Tyagi,M.B.;J.K.Thakur;D.P.Singh;A.Kumar;E.G.Prasuna;A.Kumar.
  29. J. Phycol. v.28 Taxonomic study of marine oscillatoriacean strains(cyanobacteria) with narrow trichomes.Ⅱ. Nucleotide sequence analysis of the 16S ribosomal RNA. Wilmontte,A.;S.Tumer;Y.Van de Peer;N.R.Pace.
  30. Microbiology. v.140 Evolutionary affiliation fo the marine nitrogen fixing cyanobacterium Trichosedmium sp. strain NIBB 1067, derived by 16S ribosomal RNA sequence analysis. Wilmontte,A.;J.M.Neefs;R. De Wachter.
  31. Microbiol.Rev. v.51 Bacterial evolution. Woese,C.R.