Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
권호 표지

Cross-reactivity between sera from dogs experimentally infected with Dirofilaria immitis and crude extract of Toxocara canis

  • Song, Kun-Ho (Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungnam National University) ;
  • Hayasaki, Mineo (Veterinary Clinical Center, School of Veterinary Medicine, Yamaguchi University) ;
  • Cho, Kyu-Woan (Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungnam National University) ;
  • Lee, Sang-Eun (Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Duck-Hwan (Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungnam National University)
  • Published : 2002.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was performed to investigate whethere there is cross-reactivity between Dirofilaria immitis and three intestinal nematodes of dogs. In ELISA, D. immitis- infected dog sera obtained at the 4th molting stage (9-11 weeks) and microfilaremic stage (25-30 weeks) were shown to be highly reactive with crude extract of T. canis. In immunoblotting, some antigenic fractions, 44, 57 88, 100 kDa of crude extract of T. canis, were found to be positive reaction with sera of dogs infected with D. immitis. However, little or no cross-reaction were observed between sera of D. immitis-infected dogs and crude extract antigen of T. vulpis or A. caninum. These result suggest that there are partial cross reaction between sera of D. immitis-infected dogs and the antigen of T. canis.

Keywords

References

  1. Grieve RB, Johnson MM, Jacobson RH (1981) Enzyme-linked immunosorbent assay for measurement of antibody responses to Dirofilaria immitis in experimentally infected dogs. Am J Vet Res 42: 66-69
  2. Hayasaki M (1981) Indirect hemmaglutination test for diagnosis of canine filariasis. Jpn J Vet Sci 43: 21-26
  3. Hayasaki M (1982) Reaginic and hemmaglutinating antibody production in dogs infected with Dirofilaria immitis. Jpn J Vet Sci 44: 63- 70
  4. Hayasaki M, Nakamura F, Konno K(1994) Immunoblotting analysis of somatic components of Dirofilaria immitis. J Vet Med Sci 56: 1181-1183
  5. Kaneko H, Hayasaki M, Ohishi I (1990) Antigenic identification of excretory-secretory products of adult Dirofilaria immitis. Jpn J Vet Sci 52: 995-1000
  6. Konno K, Hayasaki M (1995) Antigenic cross reactivity among Dirofilaria immitis and the four intestinal parasite-species in the dog. Jpn J Parasitol 44: 161-164
  7. Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193: 265-275
  8. Matsumura K, Kazuta Y, Endo R, Tanaka K (1984) Detection of circulating toxocaral antigens in dogs by sandwich enzyme-immunoassay. Immunology 51: 609-613
  9. Ott RA, Staples M, Weekley M, Maggio ET (1985) Demonstration of both immunogenically unique and common antigenic determinants in Dirofilaria immitis and Toxocara canis using monoclonal antibodies. Vet Immunol Immunopathol 10: 147-153