Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Effect of Aeration on Denitrification by Ochrobactrum authropi SY509

  • Song, Seung-Hoon (Interdisciplinary Program for Biochemical Engineering and Biotechnology) ;
  • Yeom, Sung-Ho (EnzyBiotech Inc., Technology Incubation Network) ;
  • Park, Suk-Soon (Department of Environmental Engineering, Semyung University) ;
  • Yoo, Young-Je (Interdisciplinary Program for Biochemical Engineering and Biotechnology, School of Chemical Engineering, Seoul National University)
  • Published : 2002.12.01
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Abstract

Aeration was found to affect the biological denitrification by Ochrobactrum authropi SY509. Although cell growth was vigorous under 1 vvm of aeration and an agitation speed of 400 rpm in a 3-L jar fermentor, almost no nitrate was removed. Yet under low agitation speeds (100, 200, and 300 rpm), denitrification occurred when the dissolved oxygen was exhausted shortly af-ter the inoculation of the microorganism. Ochrobactrum authropi SY509 was found to express highly active denitrifying enzymes under anaerobic conditions. The microorganism also synthesized denitrifying enzymes under aerobic conditions (1 vvm and 400 rpm), yet their activity was only 60% of the maximum level under anaerobic conditions and the nitrate removal efficiency was merely 15%. However, although the activities of the denitrifying enzymes were inhibited in the presence of oxygen, they were fully recovered when the conditions were switched to anaerobic conditions.

Keywords

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