Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Verification of Enhanced Phosphate Removal Capability in Pure Cultures of Acinetobacter calcoaceticus under Anaerobic/Aerobic Conditions in an SBR

  • Kim, Hyung-Jin (Central R&D Center, Samyang Corporation) ;
  • Krishna R. Pagilla (Department of Chemical and Environmental Engineering, Illinois Institute of Technology)
  • Published : 2002.12.01
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Abstract

Laboratory experiments were conducted using pure cultures of Acinetobacter under an-aerobic/aerobic cyclic conditions to explain the release and uptake of soluble phosphate in an activated sludge process showing enhanced biological phosphate removal (EBPR). Under anaerobic/aerobic cyclic conditions in a Sequencing Batch Reactor (SBR), COD uptake concurrent with soluble phosphate release by Acinetobacter was not significant during the anaerobic periods, indicating that EBPR would not be established in pure cultures. However Acinetobacter cells accumulated higher phosphate content (5.2%) in SBR than that obtained (4.3%) from batch experiments. These results suggest that Acinetobacter sp. may not follow the proposed pattern of behavior of poly-P bacteria in EBPR activated sludge Plants.

Keywords

References

  1. Wat. Res. v.25 The applied microbiology of enhanced biological phosphate removal: Accomplishments and needs. Jenkins, D.;V. Tandoi https://doi.org/10.1016/0043-1354(91)90177-R
  2. Wat. Res. v.27 Competition between polyphosphate and polysaccharide accumulating bacteria in enhanced biological phosphate removal systems. Cech, J. S.;P. Hartman https://doi.org/10.1016/0043-1354(93)90014-9
  3. Microb. Ecol. v.2 Microbiological basis of phosphate removal in the activated sludge process for the treatment of wastewater. Fuhs, G. W.;M. Chen https://doi.org/10.1007/BF02010434
  4. FEMS Microbiol. Lett. v.9 The accumulation of polyphosphate in Acinetobacter spp. Deinema, M. H.;L. H. A. Habets;J. Scholten;E. Turkstra;H. A. A. M. Webers https://doi.org/10.1111/j.1574-6968.1980.tb05652.x
  5. Wat. Res. v.19 Uptake and release of phosphate by a pure culture of Acinetobacter calcoaceticus. Ohtake, H.;K. Takahashi;Y. Tsuzuki;K. Toda https://doi.org/10.1016/0043-1354(85)90404-X
  6. Biotechnol. Bioeng. v.29 Kinetics of growth and phosphate uptake in pure culture studies of Acinetobacter species. Hao, O. J.;C. H. Chang https://doi.org/10.1002/bit.260290704
  7. Advances in Water Pollution Control: Biological Phosphate Removal from Wastewaters. Ascinetobacter sp. growth in alternate anaerobic-aerobic conditions. Tandoi, V.;M. Beccari;R. Ramadori;L. S. Annicchiarico;R. Ramadori(ed.)
  8. Wat. Res. v.32 Microbiology and biochemistry of the enhanced biological phosphate removal process. Mino, T.;M. C. M. van Loosdrecht;J. J. Heijnen https://doi.org/10.1016/S0043-1354(98)00129-8
  9. Wat. Sci. Technol. v.15 The role of microorganisms other than Acinetobacter in biological phosphate removal in activated sludge processes. Brodisch, K. E. U.;S. J. Joyner
  10. J. Ferment. Bioeng. v.69 Capacity for polyphosphate accumulation of predominant bacteria in activated sludge showing enhanced phosphate removal. Hiraishi, A.;Y. Morishima https://doi.org/10.1016/0922-338X(90)90247-T
  11. Water SA v.12 A study of selected characteristics of Acinetobacter spp. isolated from activated sludge in anaerobic/anoxic/aerobic and aerobic systems. Lotter, L. H.;M. C. Wentzel;R. E. Lowenthal;G. A. Ekama;G. V. R. Marais
  12. Standard methods for the examination of water and wastewater, (18th ed.) APHA;AWWA;WEF
  13. Wat. Sci. Technol. v.26 Uptake of organic substrates and accumulation of polyhydroxyalkanoates linked with glycolysis of intracellular carbohydrates under anaerobic conditions in the biological excess phosphate removal processes. Satoh, H.;T. Mino;T. Matsuo
  14. Biotechnol. Bioeng. v.43 Model of the anaerobic metabolism of the biological phosphorus removal process: stoichiometry and pH influence. Smolders, G. J. F.;J. van der Meij;M. C. M. van Loosdrecht;J. J. Heijnen https://doi.org/10.1002/bit.260430605
  15. Wat. Res. v.32 The behavior of polyphosphate accumulating Acinetobacter isolates in an anaerobic-aerobic chemostat. Tandoi, V.;M. Majone;J. May;R. Ramadori https://doi.org/10.1016/S0043-1354(98)00079-7
  16. Wat. Res. v.26 Contribution of precipitated phosphates and acid-soluble polyphosphate to enhanced biological phosphate removal. Appeldoorn, K. J.;A. J. Boom;G. J. J. Kortstee;A. J. B. Zehnder https://doi.org/10.1016/0043-1354(92)90200-N
  17. Proc. IAWPRC Seminar on Enhanced Biological Phosphorus Removal from Wastewaters. v.1 Phosphorus uptake by Klebsiella pneumoniae and Acinetobacter calcoaceticus. Gersberg, R. M.;D. W. Allen
  18. Wat. Sci. Technol. v.17 New strategies for the isolation of microorganisms responsible for phosphate accumulation. Suresh, N.;R. Warburg;M. Timmerman;J. Wells;M. Coccia;M. F. Roberts;H. O. Halvorson
  19. Fifth International Symposium on Microbial Ecology. Polyphosphate accumulating bacteria and its ecological characteristics in activated sludge process. Nakamura, K.;K. Masuda;E. Mikami
  20. Wat. Res. v.34 Competitive growth of Nocardia and Acinetobacter under anaerobic/aerobic batch operation. Kim, H.;K. R. Pagilla https://doi.org/10.1016/S0043-1354(00)00030-0
  21. Ph. D. Dissertation The effects of temperature and mean cell residence time on enhanced biological phosphorus removal by activated sludge. Mamais, D.