Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Analysis of Antifungal Proteins in Streptomyces sp. SAR01

Streptomyces sp. SAR01 균주에서의 항진균 관련 단백질 분석

  • 이영근 (한국원자력연구소, RI 방사선응용연구팀) ;
  • 김재성 (한국원자력연구소, RI 방사선응용연구팀) ;
  • 조규성 (한국원자력연구소, RI 방사선응용연구팀) ;
  • 장병일 (한국원자력연구소, RI 방사선응용연구팀) ;
  • 추철형 (한국원자력연구소, RI 방사선응용연구팀)
  • Published : 2002.09.01
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Abstract

To analyze proteins related to antifungal activity, SAR01 strain was isolated from seaweed and identified as Streptomyces sp. from the result of FAME (fatty acid methyl ester) analysis. The isolated strain had antifungal activities against T species of plant pathogenic fungi. Antifungal activity deficient mutant (SAR 535) of Streptomyces sp. SAR01 was induced by gamma radiation $(^{60}Co,\;5kGy)$. By 2 D electrophoresis analysis, 6 protein spots were found in wild strain (SAR01) but these spots disappeared in mutant strain (SAR535). Among them, 5 proteins showed similarities to heat shock protein 70(HSP70), Fe-containing superoxide dismutase II (Fe- SODII), ribosome recycling factor (RRF), 10 kDa chnperonin (GroES) and inorganic pyrophosphatase (PPAse), respectively. It suggested that the above 6 proteins could be closely related to the antifungal activity of Streptomyces sp. SAR01.

항진균 활성 관련 단백질을 탐색하기 위해 미역류로부터 식물병원성 곰팡이의 생장을 저해하는 SAR01 균주를 분리하였고, FAME (fatty acid methyl ester) 분석 결과, Streptomyces sp.로 동정되었다. 방사선 조사$(^{60}Co)$를 실시한 결과, Botrytis cinerea를 포함한 5종의 식물병원성 곰팡이에 대한 항진균 활성을 소실한 SAR535 균주 외 6종의 돌연변이 균주가 유도되었다. SAR01 야생형 균주와 SAR535 돌연변이 균주의 세포내 단백질의 이차원 전기영동 분석결과, 6종의 단백질이 야생형 균주인 SAR01 균주의 세포내에만 존재하였다. 이들 6종의 단백질 중, 5종은 heat shock protein 70 (HSP70), Fe-containing superoxide dismutase II (Fe - SODII), ribosome recycling factor (RRF), 10 kD chaperonin (GroES) 및 inorganic pyrophosphatase (PPAse)와 각각 75%, 93%, 100%, 96% 및 83%의 유사성을 보였다. 이들 6종의 단백질들은 Streptomyces sp. SAR01 균주의 항진균 활성과 밀접한 관계가 있을 것으로 사료된다.

Keywords

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