Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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Effects of Dietary Levan on Adiposity, Serum Leptin and UCP Expression in Obese Rats Fed High Fat Diet

고지방 식이로 유도된 비만쥐에서 식이 레반이 체지방 형성 및 혈청 렙틴과 UCP 발현에 미치는 영향

  • 강순아 (경희대학교 동서의학대학원 임상영양전공) ;
  • 홍경희 (경희대학교 동서의학대학원 임상영양전공) ;
  • 김소혜 (경희대학교 동서의학대학원 임상영양전공) ;
  • 장기효 (경희대학교 동서의학대학원 임상영양전공) ;
  • 김철호 (㈜리얼바이오텍, 생명공학연구원) ;
  • 조여원 (경희대학교 임상영양연구소)
  • Published : 2002.11.01
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Abstract

The effects of dietary levan, high-molecular-weight $\beta$-2,6-linked fructose polymer, on adiposity, serum leptin and UCP expression in rats fed high fat diet were studied. The adipose tissue hormone, leptin has been proposed to be involved in the regulation of food intake and energy expenditure. Uncoupling protein (UCP), a mitochondrial protein that uncouples the respiratory chain from oxidative phosphorylation, generates heat instead of ATP, thereby increase energy expenditure. To determine whether the dietary levan may have the anti-obesity effect, 4 wk old Sprague Dawley male rats fed high fat diet for 6 wks to induce obesity, and subsequently fed one of three diets for further 6 wks: 1) high fat (40% of calories) diet without levan 2) with 3% (w/w) levan 3) with 5% levan. For the comparison, control group fed AIN-76A diet. Visceral and peritoneal fat masses were lower in high fat diet with levan groups compared to high fat diet group. The effect of levan was dose-dependent. Adipocyte size was significantly reduced in the levan diet groups compared to the no levan diet group. Serum cholesterol level was not affected by levan containing diet, while the serum HDL cholesterol level was higher in leven diet groups. In addition, serum triglyceride level was markedly reduced by levan containing diet, thus lower than that of control group. Serum leptin was reduced by levan containing diet and lower in 5% levan group compared to 3% levan group (p < 0.001), as a result, serum leptin and insulin levels of 5% levan group were reduced to level of control group. Futhermore, the serum leptin level reflected the adiposity. The expression of UCP 1, and UCP 2 in brown adipose tissue was up-regulated by levan containing diet. In conclusions, levan containing diet reduced adiposity and serum triglyceride but increased UCP expression in the obese rats fed high fat diet. (Korean J Nutrition 35(9) : 903~911, 2002)

Keywords

References

  1. Am J Clin Nutr v.67 no.SUP. Multi-factorial causation of obesity : implications for prevention Grundy, SM.
  2. Obesity Solutions: Report of a Meeting Nutr Rev v.55 no.5 Albu J;Allison D;Boozer CN;heymsfield S;Kissileff H;Kretser A;Krumhar K;Leibel R;Nonas C;Pi-Sunyer X;Vanltalllie T;Wedral E.
  3. Annu Rev Nutr v.18 Dietary fructans Roverfroid MB;Delzenne NM. https://doi.org/10.1146/annurev.nutr.18.1.117
  4. Biopolymers v.5 Polysaccharides 1. Levan Rhee SK;Song KB;Kim CH;Park BS;Jang EK;Jang KH;Erick Vandamme(ed.);Sophie De Baets(ed.);Alexander Steinbuchel(ed.) https://doi.org/10.1002/bip.1967.360050404
  5. Adv appl Microbiol v.35 Microbial levan Han YW. https://doi.org/10.1016/S0065-2164(08)70244-2
  6. J Nutr Biochem v.10 In vitro digestibility and fermentability of levan and its hypocholeserolemic effects in rats Yamamoto Y;Takahashi Y;Kawano M;Iizuka M;Matsumoto T;Saeki S;Yamaguchi H. https://doi.org/10.1016/S0955-2863(98)00077-1
  7. Int J Biol Macromol v.27 Molecular weight and antitumor activity of Zymomonas mobilis levan Calasans, GMT;Lima RC;de Franca FP;Lopes CE. https://doi.org/10.1016/S0141-8130(00)00125-2
  8. Carbohydr Polym v.23 Solution properties of levan polysaccaride form Pseudomonas syringae pv. phaseolicola, and its possible primary role as a blocker of recognition during pathogenesis Kasapis S;Morris ER;Gross M;Rudolph K.
  9. Korean J Nutrition v.34 no.8 Effects of high fat diet on serum leptin and insulin level and brown adipose tissue UCP 1 expression in rats Hong KH;Kang SA;Kim SH;Choue RW.
  10. Proc Nutr Soc v.59 no.3 Role of adipose tissue in body-weight regulation: mechanisms regulating leptin production and energy balance Havel PJ. https://doi.org/10.1017/S0029665100000410
  11. FEBS Lett v.438 The mechanism of proton transport mediated by mitochondrial uncoupling proteins Keith DG;Martin J;Pet J. https://doi.org/10.1016/S0014-5793(98)01246-0
  12. Am J Physiol endocrinol Metab v.279 no.2 Effect of high-fat diet, surrounding temperature, and enterostatin on uncoupling portein gene expression Rippe C;Berger K;Boeirs C;Ricquier D;Erlanson AC.
  13. FEBS Lett v.418 Cloning of rat uncoupling protein-3 and uncoupling protein-2 cDNAs: their gene expression in rats fed a high fat diet Matsuda J;Hosoda K;Itoh H;Son C;Doi K;Tanaka T;Fukunaga T;Inoue G;Nishimura H;Yoshimass YI;Yamori Y;Nakao K. https://doi.org/10.1016/S0014-5793(97)01381-1
  14. FEBS Lett v.408 Uncoupling protein-3: a new member of the mitochondrial carrier family with tissue-specific expression Boss O;Samec S;Giacobino AP;Rossier C;Dulloo a;Seydoux J;Muzzin P;Giacobino JP. https://doi.org/10.1016/S0014-5793(97)00384-0
  15. Nutr Metab Cardiobasc Dis v.11 no.SUP. 4 Study of the regulation by nutrients fo the expression genes involved in liogenesis and obesity in humans and animals Delzenne N;Ferre P;Beylot M;Daubioul C;Declercq B;Diraison F;Dugali I;Foufelle F;Foretz M;mace K;Reimer R;Plamer G;Rutter G;Tavara J;Van Loo J;Vidal H.
  16. J Nutr v.130 Dietary oligo-fructose lessens hepatic steatosis, but dose not prevent hypertriglyceridemia in obese Zucer rats Daubioul CA;Taper HS;Wispelaere LD;Delzenne NM.
  17. Nutr Res v.22 Effect of a long-chain fructan Raftline HP on blood lipids and spontaneous atherosclerosis in low density receptor knockout mice Motrensen A;Poulsen M;Frandsen H. https://doi.org/10.1016/S0271-5317(02)00358-5
  18. Adv Ther v.15 no.5 Four week supplementation with a nutural dietary compound produces favorable changes in body composition Hoeger WW;harris C;Long EM;Hopking DR.
  19. J Bio Chem v.273 no.1 Uncoupling protein 3 expression in rodent skeletal muscle is modulated by food intake but not by changes in environmental temperature Boss O;Samec S;Khune F;Bijlenga P;Assimacopoulos-Jeannet F;Seydoux J;Giacobino JP;Muzzin P. https://doi.org/10.1074/jbc.273.1.5
  20. J Nutr v.129 Allyl-containing sulfide in garlic increase uncoupling protein content in brown adipose tissue, and noradrenaline and adrenaline secretion in rats Oi Y;Kawada T;Shishido C;Wada K;Kominato Y;Nishimura S;Ariga T;Iwai K.
  21. J Nutr v.131 Chronic docosahexaenoic acid intake enhances expression of the gene for uncoupling protein 3 and effects pleiotropic mRNA levels in skeletal muscle in aged C57BL/6NJcl mice Cha SH;Fukushima A;Sakuma K;Kagawa Y.
  22. Proc Soc Exp Bio Med v.156 A reliable photomicrographic method for determining fat cell size and number: application to dietary obesity Lavau M;Susin C;Knittle J;Blanchet-HS;Greenwood MRC
  23. J Nutr Biochem v.12 Changes in UCP mRNA expression levels in brown adipose tissue and skeletal muscle after feeding a high-energy diet and relationships with leptin, glucose and PPARy Javier M;Amelia MJ;Alfredo M. https://doi.org/10.1016/S0955-2863(00)00131-5
  24. Lipids v.30 no.2 Dietary oligofructose lowers triglycerides, phopholipids and cholesterol in serum and very low density lipoproteins of rats Flordallso M;Kok N;Desager JP;Goethals F;Deboyser D;Roberfroid M;Delzenne N. https://doi.org/10.1007/BF02538270
  25. Metabolism v.45 no.12 Dietary oligofructose modifies the impact of fructose on hepatic triacylglycerol metabolism Kok N;Reverfroid M;Delzenne N. https://doi.org/10.1016/S0026-0495(96)90186-9
  26. J Appl Tosicol v.18 no.1 Oligofructose Modulates lipid metabolism alterations induced -by a fat-rich in rats Kok NN;Taper HS;Delzenne NM. https://doi.org/10.1002/(SICI)1099-1263(199801/02)18:1<47::AID-JAT474>3.0.CO;2-S
  27. J Nutr v.127 no.SUP. 7 Biochemical basis of oligofructose-in-duced hypolipidemia in animal models Delzenne NM;Kok NN.
  28. J Nutr v.130 Platycodi radix affects lipid metabolism in mice with high fat diet-induced obesity Han LK;Xu BJ;Kimura Y;Zheng Y;Okuda H.
  29. Nutr Res v.20 no.2 Effects of dietary inulin on serum lipids, blood glucose and the gastrointestinal environment in hypercholesterolemic men Causey JL;Feirgat JM;Gallaher DD;Tungland BC;Slavin JL. https://doi.org/10.1016/S0271-5317(99)00152-9
  30. Br J Nutr v.82 no.1 The effect of the daily intake of inulin on fasting lipid, insulin and glucose concentration in middle=aged emn and women Jackson KG;Taylor GR;Clohessy AM;Willians CM. https://doi.org/10.1017/S0007114599001087
  31. J Nutr v.129 no.SUP. Effects of dietary inulin on serum lipids Davidson MH;Maki KC.
  32. Diabetes Care v.19 Insulin resistance and ovesity: the role of fat distribution Abate N. https://doi.org/10.2337/diacare.19.3.292
  33. New Eng J Med v.334 no.5 Serum immunoreactive-leptin concentrations in normal-weight and obese humans Considine RV;sinha MK;Heiman ML;Kriauciunas A;Stephens TW;Nyce MR;OHannesian JP;Marco CC;Mckee LJ;Bauer Tl:Caro FJ. https://doi.org/10.1056/NEJM199602013340503
  34. Biomed & Pharmacother v.51 Regulation of ob gene and overexpression in obesity Guerre-Millo M. https://doi.org/10.1016/S0753-3322(97)88048-1
  35. J Clin Endo Met v.81 no.12 Relationship of plasma leptin to plasma insulin and adiposity in normal weight and ovrweight women: effects of dietary fat content and sustained weight loss Havel PJ;Kasim KS;Mueller W;Johnson PR;Gingerich RL;Stern JS. https://doi.org/10.1210/jc.81.12.4406
  36. J Lipid Res v.38 no.10 Uncoupling protein gene: quantification of expression levels in adipose tissues of obese and non-obese humans Oberkofler H;Dallinger G;Liu YM;Hell E;Krempler F;Patsch W.
  37. Hand Bood of Obesity Brown Adipose tissue Himms-Hagen J;Ricquier D;Bray GA(ed.);Brouchard C(ed.);James WPT(ed.)