한국수정란이식학회지 (Journal of Embryo Transfer)

  • 제17권2호
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  • Pages.109-115
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  • 2002
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  • 2508-755X(pISSN)
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  • 2288-0178(eISSN)
한국수정란이식학회 (The Korean Society of Embryo Transfer)
권호 표지

외부유전자가 도입된 체세포를 이용한 소 형질전환 복제란 생산

Production of Bovine Transgenic Embryos Derived from Non-transfected and Transfected Adult Cells

  • J. K. Cho (Department of Theriogenology & Biotechnology College of Veterinary Medicine, Seoul National University) ;
  • M.M.U. Bhuiyan (Department of Theriogenology & Biotechnology College of Veterinary Medicine, Seoul National University) ;
  • G. Jang (Department of Theriogenology & Biotechnology College of Veterinary Medicine, Seoul National University) ;
  • Park, E. S. (School of Agriculutral Biotechnology, Seoul National University) ;
  • J. M. Lim (School of Agricultural Biotechnology, Seoul National University) ;
  • S. K. Kang (Department of Theriogenology & Biotechnology College of Veterinary Medicine, Seoul National University) ;
  • Lee, B. C. (Department of Theriogenology & Biotechnology College of Veterinary Medicine, Seoul National University) ;
  • W. S. Hwang (Department of Theriogenology & Biotechnology College of Veterinary Medicine, Seoul National University)
  • 발행 : 2002.08.01
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초록

The present study was conducted for the production of transgenic cloned cows those secrete human lactoferricin into milk by somatic cell nuclear transfer (NT). To estimate detrimental effects of gene transfection on transgenic cloned embryo production, development rates of NT embryos were compared between transfected and non-transfected cumulus and ear fibroblast cells. An expression plasmid for human lactofericin (pbeta-LFC) was constructed by inserting a bovine beta-casein promoter, a green fluorescent protein (GFP) marker gene, and human lactoferricin target gene into a pcDNA3 plasmid. Two bovine somatic cell lines (cumulus cell and ear fibroblast) were established and transfected with the expression plasmid using a liposomal transfection reagent, Fugene6 as a carrier. Cumulus cell and ear fibroblast were transfected at the passage of 2 to 4, trypsinized and GFP-expressing cells were randomly selected and used for somatic cell NT. Developmental competences (rates of fusion, cleavage, and blastocyst formation) in bovine transgenic somatic cell NT embryos reconstructed with non-transfectecd cells were significantly higher than those from transfected cells in cumulus cell and ear fibroblast (P＜0.05). This study indicated that transfection of done. cell has detrimental effect on embryo development in bovine transgenic NT.

키워드

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