한우 및 한국재래산양 유래 말초혈액 림프구의 미소핵을 이용한 방사선 피폭의 생물학적 선량측정

Use of Frequencies of Micronuclei as Biological Dosimetry in Korean Native Cattle and Goat Lymphocytes after Irradiation in vitro

  • 발행 : 2002.09.01

초록

The frequencies of gamma-ray-induced micronuclei (MN) in cytokinesis-blocked (CB) lymphocytes at several doses were measured in three donors of Korean native cattle and Korean native goat. Measurements performed after irradiation showed a dose-related increases in MN frequency in each of the donors studied. When analysed by linear-quadratic model the line of best fit was cattle : y : 0.1016D +$0.0118D^2$+0.0147, goat : y = 0.1353D +$0.0043D^2$+0.0087 (y : number of MN/CB cells and D = irradiation dose in Gy). The relative sensitivity of goat lymphocytes compared with cattle lymphocytes was estimated by best fitting linear-quadratic model based on the radiation-induced MN data over the range from 0 Gy to 4 Gy. In the case of MN frequency with 0.05, 0.1, 0.2, 0.4 and 0.8, the relative sensitivities of goat lymphocytes were 1.106. 1.166. 1.140, 1.069 and 0.976 respectively. Our in vitro radiobiological study confirmed that the cytogenetic response obtained in blood from cattle and goat can be utilized for application in environmental studies.

키워드

참고문헌

  1. Appl Radiat Isot v.38 The present state and perspectives of micronucleus assay in radiation protection. A review Almassy Z;Krepinsky AB;Bianci A(et al.) https://doi.org/10.1016/0883-2889(87)90033-5
  2. Environ Mol Mutagen v.37 Human micronucleus project: international database comparison for results with the cytokinesis-block micronucleus assay in human lymphocytes: I. effect of laboratory protocol, scoring criteria, and host factors on the frequency of micronuclei Bonassi S;Fenech M;Lando C(et al.) https://doi.org/10.1002/1098-2280(2001)37:1<31::AID-EM1004>3.0.CO;2-P
  3. Radiat Res v.49 Radiation-induced human chromosome aberration yields following an accidental whole-body exposure to 60Co gamma-rays Brewen JG;Preston RJ;Littlefield LG https://doi.org/10.2307/3573421
  4. Mutat Res v.455 The in vitro micronucleus technique Fenech M https://doi.org/10.1016/S0027-5107(00)00065-8
  5. Bone Marrow Trans v.3 Blood cell changes after radiation exposure as an indicator for hemopoietic stem cell function Fieldner TM;Nothdurft W;Steinbach KH
  6. Mutat Res v.372 Both bovine and rabbit lymphocytes conditioned with hydrogen peroxide show an adaptive response to radiation damage Flores MJ;Pinero J;Ortiz T(et al.) https://doi.org/10.1016/S0027-5107(96)00082-6
  7. Radiat Res v.123 Changes after irradiation in the number of mitotic cells and apoptotic fragments in growing mouse hair follicles and in the width of their hairs Geng L;Potten CS https://doi.org/10.2307/3577660
  8. UNSCEAR Report Acute radiation effects in victims of the Chernobyl nuclear power plant accident Guskova AK;Barabanova AV;Baranov AY(et al.)
  9. Biomed Environ Sci v.13 Monitoring of human exposure to radiation with the binucleated lymphocyte micronucleus assay He JL;Jin HY;Jin LF(et al.)
  10. IAEA-SM-199/4 Biological dosimetry with particular reference to chromosome aberration analysis. A review of methods IAEA
  11. Technical report 260 Biological dosimetry : chromosomal aberration analysis for dose assessment IAEA
  12. Korean J Vet Res v.27 Relationships between radiation-induced prostaglandin E2 and natural killer cell activity in mice Kim SH;Cho CK;Kim TH(et al.)
  13. Anticancer Res v.13 Frequency of micronuclei in lymphocytes following gamma and fast-neutron irradiations Kim SH;Cho CK;Kim TH(et al.)
  14. Anticancer Res v.16 Measurement of apoptotic fragments in growing hair follicles following r-ray irradiation in mice Kim TH;Kim SH;Kim JH(et al.)
  15. Radiat Res v.124 The persistence of lymphocytes with dicentric chromosomes following whole-body X irradiation of mice Klingermem AD;Halperin EC;Erexson GL(et al.) https://doi.org/10.2307/3577689
  16. Radiation-induced chromosome damage in man Cytogenetic effects of ionizing radiations in somatic cells from experimental mammals and extrapolation to man Leonard A;Ishihara T(ed.);Sasaki M(ed.)
  17. Int J Radiat Biol v.53 Modulation of radiation-induced chromosome aberrations by DMSO, an OH radical scavenger, 1. Dose-response studies in human lymphocytes exposed to 220 kV X-rays Littlefield LG;Joiner EE;Colyer SP(et al.) https://doi.org/10.1080/09553008814551241
  18. Biological Dosimetry An overview of radiation dosimetry by conventional cytogenetic method Lloyd DC;Eisert WG(ed.);Mendelsohn ML(ed.)
  19. Int J Radiat Biol v.59 Biological indications for radiation damage M ller W-U;Streffer C https://doi.org/10.1080/09553009114550771
  20. Radiat Res v.99 The use of peripheral blood mononuclear cell prematurely condensed chromosomes for biological dosimetry Pantelias GE;Maillie HD https://doi.org/10.2307/3576452
  21. Int J radiat Biol v.57 Hair medullary cell counts : a simple and sensitive indicator of radiation exposure Potten CS;Geng L;Taylor P https://doi.org/10.1080/09553009014550301
  22. Radiat Protect Dosimetry v.25 Dose assesments by cytogenetic analysis in the Goiania(Brasil) radiation accident Ramalho AJ;Nascimento ACH;Natorajan AT https://doi.org/10.1093/oxfordjournals.rpd.a080358
  23. Mutat Res v.207 Use of frequencies of micronuclei as quantitative indicators of X-ray-induced chromosome aberrations in human peripheral blood lymphocytes: comparison of two methods Ramalho A;Sunjevaric I;Natarajan AT https://doi.org/10.1016/0165-7992(88)90078-4
  24. Mutat Res v.289 Measurement of micronuclei by cytokinesis-block method in bovine lymphocytes Scarfi MR;Lioi MB;Di Berardino D(et al.) https://doi.org/10.1016/0027-5107(93)90080-Y
  25. Nature v.278 Homogeneous sensitivity of human peripheral blood lymphocytes to radiation-induced chromosome damage Scott D;Lyons CY https://doi.org/10.1038/278756a0
  26. Health Phys v.44 Biologically and physiologically recorded doses after an accidental exposure to $^{60}Co$ gamma-rays Stenphan G;Hadnagy W;Hammermaier C(et al.)
  27. Mutagenesis v.15 Cytogenetic monitoring of hospital workers occupationally exposed to ionizing radiation using the micronucleus centromere assay Thierens H;Vral A;Morthier R(et al.) https://doi.org/10.1093/mutage/15.3.245
  28. Mutagenesis v.3 The identification of micronucleated chromosomes: a possible assay for aneuploid Thomson EJ;Perry PE https://doi.org/10.1093/mutage/3.5.415
  29. Report : Sources and effects of ionizing radiation, Annex G, Early effects in man of high doses of radiation, chapter III : prognotic indicators and biological dosimetry UNSCEAR
  30. 방사선방어학회지 v.17 KCCH cyclotron neutron 및 $^{60}$Co γ-ray에 의한 인체 말초혈액 임파구의 염색체 이상 측상 김성호;김태환;정인용(등)
  31. Low dose radiation : Biological bases of risk assessment The genetic effects of the atomic bombs: probloms in extrapolating from somatic cell findings to risk for children Neel JV;Schull WJ;Awa AA(et al.);Baverstock KF(ed.);Stather JW(ed.)