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Antioxidant Activity of Grape Seed Ethanol Extract

포도종실 에탄올 추출물의 항산화 활성

  • 정하열 (한경대학교 식품공학과 및 식품생물산업연구소) ;
  • 윤수정 (한경대학교 식품공학과 및 식품생물산업연구소)
  • Published : 2002.10.01

Abstract

To examine the antioxidant activity of grape seed ethanol extract, the antioxidative index (AI) by the active oxygen method (AOM) and peroxide value (POV) of linoleic acids containing the extract at levels of 100, 500, 1000 ppm was measured during storage at 6$0^{\circ}C$ for 12 days. When comparing with BHT, the extract at levels of 500 ppm showed similar or better antioxidant activity (AI: 2.25, POV: 57 meq/kg oil) than that (AI: 1.21, POV:58 meq/kg oil ) of BHT at 200 ppm level. The mixture of 500 ppm of the extract and 500 ppm of ascorbic acid showed intense synergistic antioxidant activity (AI: 6.21, POV: 14 meq/kg oil) compared with using 1000 ppm of the extract only (AI: 3.39, POV 43 meq/kg oil). Also to determine the feasibility of using the extract for natural antioxidant, the oxidative stability of roasted peanut and Ramyon was investigated by measuring the POV of crude oils from the samples stored at 60"C for 18 days. The oxidative stability of roasted peanut and Ramyon seemed to be enhanced by treatment with the extract at level of 1000 ppm, especially with the 1 : 1 mixture of extract and ascorbic acid. This study suggested that grape seed ethanol extract could be used as the natural antioxidant for the improvement of overall oxidation stability of fat containing foods.oods.

포도종실 에탄을 추출물의 항산화효과를 조사하기 위하여 각각 100, 500, 1000 ppm의 농도로 리놀산에 첨가한 후 6$0^{\circ}C$ 항온기에 저장하면서 항산화지수와 과산화물가를 측정한 결과 hS는 각각 1.4, 2.25, 3.39, POV는 71, 57, 43 meq/kg oil로서 첨가량이 500ppm이상인 경우에는 BHT 200 ppm 첨가구(AI: 1.21, POV: 58 meqAg oil)와 대등하거나 보다 우수한 산화 억제 효과를 확인할 수 있었다. 포도종실 에탄올 추출물 500 ppm에 아스코르빅 산 500 ppm을 혼합하여 병용한 첨가구의 AI는 6.21,12일 저장 후 POV는 14 meq/kg oil로서 포도종실 에탄을 추출물 500ppm이나 1000ppm만을 첨가한 시료에 비하여 항산화 효과가 상승됨을 확인할 수 있었다. 볶은 땅콩이나 라면에 포도종실 에탄을 추출물 1000 ppm을 단독으로 혹은 아스코르빅 산과 500 ppm씩 병용하여 처리하고 6$0^{\circ}C$에서 18일간 저장하며 시료로부터 추출한 조지방질의 POV를 측정한 결과, 볶은 땅콩이나 라면의 산화안정성이 향상되는 것을 확인할 수 있어서 포도종실 에탄올 추출물은 지방질 함유 가공식품의 산화안정성 향상을 위한 천연 항산화제로서 사용될 수 있음을 알 수 있었다.

Keywords

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