생명과학회지 (Journal of Life Science)

  • 제12권5호
  • /
  • Pages.605-609
  • /
  • 2002
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

GTG, High-Resolution, Nor-banding에 의한 개의 염색체 분석

Chromosome Analysis by GTG, High-Resolution, and NOR-banding Techniques in the Dog (Cams familaris)

  • 김종봉 (대구가톨릭대학교 자연대학 생물학과) ;
  • 윤인숙 (대구보건대학 임상병리학과)
  • 발행 : 2002.10.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

개의 염색체수는 2n=78이며 염색체의 형태를 보면 submetacentric chromosome metacentric chromosome인 X와 Y를 제외하고 모든 상염색체가 acrocentric chromosome이며 그 크기가 작고 크기의 차이가 나지 않는 염색체들이 많아 G-banding등에 의한 핵형의 표준화가 완전하게 이루어지고 있지 않다. 본 연구fl서는 G-banding high-resolution-banding NOR- banding등의 방법을 이용하여 개의 미소 염색체들에 대한 핵형 및 염색체 동정을 하고자 하였다. C-banding과 high-resolution banding에 의한 그 결과 아직 표준화가 되어 있지 않은 염색체 중 34번, 37번 등의 염색체에서 본 연구 결과가 차이가 있었으나 이는 해석상의 차이로 보이며 NOR-banding에 의한 분석결과 Y염색체와 3쌍의 상염색체에서 존재함을 확인 할 수 있었고 이는 개의 염색체 동정의 지표로 활용할 수 있으리라고 생각된다.

None of the numerous published canine idiograms and karyotypes has yet been generally accepted as a standard one because the dog has 76 acrocentric autosomes of similar size and shape. To establish canine banded karyotype from the 22nd chromosome to the 37th chromosome, we analyzed canine chromosomes by GTG, high resolution, and NOR-banding techniques. The GTG and high resolution banding patterns of canine chromosomes corresponded to other reports described previously except for a few chromosomes. While other researchers observed 12 bands, we observed 7 bands in the banding patterns of chromosome 24, 34 and 37. On the other hand, the banding patterns by NOR-banding technique showed that three pairs of autosomes have nucleolus organizer regions at the terminal ends of their long arm, and the Y chromosome has it in its short arm terminal. However, the X chromosome has no nucleolus organizer like other mammals.

키워드

참고문헌

  1. J. Heredity v.90 no.1 FISH Mapping and Identification of Canine Chromosomes Breen, M.;C.F. Langford;N.P. Carter;N.G. Holmes;H.F. Dickens;R. Thomas;N.Suter;E.J. Ryder;M. Pope;M.M. Binns https://doi.org/10.1093/jhered/90.1.27
  2. Chromosome Res. v.7 The DAPI banded karyotype of the domestic dog(Canis Familiaris) generated using chromosome-specific paint probes Breen, M.;J. Bullerdiek;C.F. Langford https://doi.org/10.1023/A:1009224232134
  3. Cytogenet. Cell Genet. v.12 Committee for a Standardized Karyotype of Rattus norvgicus 1973: Standard karyotype of the Norway rat(Rattus norvegicus) https://doi.org/10.1159/000130455
  4. J. Heredity v.63 Commettee on Standardixed Genetic Nomenclature for Mice 1973 : Standard karyotype of the mouse (Mus musceles)
  5. Jpn. I Vet Sci. v.51 The banding patterns of mormal canine chromosomes Fujinaga, T.;M. Yamashita;M.C. Yoshida;S. Mizuno;M. Tajima;Y. Okamoto https://doi.org/10.1292/jvms1939.51.294
  6. Hereditas v.51 The chromosomes of the dog. Gustavsson, I. https://doi.org/10.1111/j.1601-5223.1964.tb01926.x
  7. Experimentia v.36 Controlled silver staining of nucleolus organijer regions with protective colloidal developer ; 1-step method Howeel, W. M. ;D. A. Black https://doi.org/10.1007/BF01953855
  8. An international system for human cytogenetic monenclature Basel, S. Karger;Mitelman, F.(eds.)
  9. Cytogenet Cell Genet v.53 International system for cytogenetic nomenclature of domestic animals Di Bernardino(ed.);D. H. Hayes(ed.);R. Fries(ed.);S. Long(ed.) https://doi.org/10.1159/000132898
  10. Korean J. Genetics v.8 no.4 G-banded karyotype of Korean Sindo dog(Canis familaris) Kim, J. B.;H. S. Ok
  11. Can. J. Genet. Cytol. v.18 Banding analysis of the somatic chromosomes of the domestic dog(Canis familiaris) Manolache, M.;W.M. Ross;M. Schmid
  12. Cytogenet. Cell Genet. v.34 Heterochromatin, synaptonemal complex, and NOR activity in the somatic and germ cells of a male domestic dog, Canis familiars(Mammalia, Canidae) Pathak, S.;P.V. Tuinen;D.E. Merry https://doi.org/10.1159/000131799
  13. J. Heredity v.90 no.1 Working with Canine Chromosome: Current Recommendations for karyotype description Reimann, N.;S. Bartnitzke;I. Nolte;J. Bullerdiek https://doi.org/10.1093/jhered/90.1.31
  14. Cytogenet. Cell Genet. v.73 An extended nomenclature of the canine karyotype Reimann, N.;S. Barnitzke;J. Bullerdiek;U. Schmitz;P. Rogalla;I. Nolte;M. Ronne https://doi.org/10.1159/000134326
  15. Cancer Genet. Cytogenet. v.90 Trisomy 18 in a canine thyroid adenoma Reimann, N.;U. Bonk;M. Werner;J. Bullerdiek;S. Bartmizke https://doi.org/10.1016/S0165-4608(96)00045-3
  16. Cytogenet. Cell Genet v.15 The Giemsa banding pattern of the canine karyotype Selden, J.R.;P.S. Moorhead;M. L. Oehlert;D.F. Patterson https://doi.org/10.1159/000130537
  17. Lancet. v.2 Rapid banding technique for human chromosome Seabright, M.
  18. Genome. v.34 The Giemsa banding pattern of canine chromosomes using a cell synchronization technique Stone, D. M.;P. B. Jacky;D. J. Prieur https://doi.org/10.1139/g91-062
  19. Chromosome Res. v.4 Report on the progress of the standardization of the G-banded canine(Canis familiaris)karyotype Switonski, M.;N. Reimann;A.A. Bosma;S. Long;S. Bartnitzke;A. Pienkowska;M.M. Moreno-Milan;P. Fischer https://doi.org/10.1007/BF02263682