생명과학회지 (Journal of Life Science)

  • 제12권5호
  • /
  • Pages.561-565
  • /
  • 2002
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

페놀분해세균인 Pseudomonas sp. EL-04J로부터 Trichloroethylene 분해효소의 확인

Confirmation of Trichloroethylene-Degrading Enzyme from a Phenol-Degrading Bacterium, Pseudomonas sp. EL-04J

  • 박근태 (부산대학교 미생물학과) ;
  • 김호성 (부산대학교 미생물학과) ;
  • 손홍주 (밀양대학교 생물공학과) ;
  • 이건 (부산대학교 미생물학과) ;
  • 박성훈 (부산대학교 응용화학공학부 및 환경기술산업개발연구소) ;
  • 이상준 (부산대학교 미생물학과)
  • Park, Geun-Tae (Department of Microbiology, Pusan National University) ;
  • Kim, Ho-Sung (Department of Microbiology, Pusan National University) ;
  • Son, Hong-Ju (Department of Biotechnology, Miryang National University) ;
  • Lee, Gun (Department of Microbiology, Pusan National University) ;
  • Park, Sung-Hoon (Division of Chemical Engineering, and Institute for Environmental Technology) ;
  • Lee, Sang-Jun (Department of Microbiology, Pusan National University)
  • 발행 : 2002.10.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Pseudomonas sp. EL-041는 페놀로 적응된 활성오니로부터 분리되었으며, 페놀을 분해할 수 있는 동시에 trick-loroethylene(TCE)을 공동대사할 수 있는 세균이다. 본 연구에서는 Pseudomonas sp. EL-041에서 발현되는 trick-loroethylene 분해효소의 종류를 효소활성 측정 및 specific phenol oxygenase gene의 DNA sequencing을 통하여 조사하였으며, 그 결과 본 균주의 trichloroethylene 분해효소는 monooxygenase였으며, phenol hydroxylase로 추정되었다.

Pseudomonas sp. EL-041 was previously isolated from phenol-acclimated activated sludge. This bacterium was capable of degrading phenol and cometabolizing trichloroethylene (TCE). In this study, we report the identification of trichloroethylene- degrading enzyme in Pseudomonas sp. EL-041 by the investigation of enzyme activity and DNA sequencing of specific phenol oxygenase gene. As the results of experiment, trichloroethylene-degrading enzyme in Pseudomonas sp. EL-041 was monooxygenase and suspected to phenol hydroxylase.

키워드

참고문헌

  1. Microbiol. Rev. v.58 Bacterial dehalogenases: biochemistry, genetics, and biotechnological applications Fetzner, S.;F. Lingens
  2. Appl. Environ. Microbiol. v.56 Phenol and trichloroethylene degradation by Pseudomonas cepacia G4: kinetics and interactions between substrates Folsom, B. R.;P. J. Chapman;P. H. Pritchard
  3. J. Fermen. Bioeng. v.79 Trichloroethylene degradation by genetically engineered bacteria carrying cloned phenol catabolic genes Fujita, M.;M. Ike;J. Hioki;K. Kataoka;M. Takeo https://doi.org/10.1016/0922-338X(95)94075-3
  4. J. Bactriol. v.176 Efficient degradation of trichloroethylene by a hybrid aromatic ring dioxygenase Furukawa, K.;J. Hirose;S. Hayashida;K. Nakamura https://doi.org/10.1128/jb.176.7.2121-2123.1994
  5. Appl. Environ. Microbiol. v.45 Trichloroethylene degradation by two independent aromatic degrading pathway in Alcaligenes eutrophus JMP 134 Haker, A. R.;Y. Kim
  6. J. Kor. Environ. Sci. Soc. v.10 Isolation and characterization of aerobic trichloroethylene cometabolizing bacterium Kim, H. S.;G. T. Park;H. J. Son;S. H. Park;S. J. Lee
  7. J. Kor. Environ. Sci. Soc. v.10 Cometabolism of trichloroethyene by a phenol-degrading bacterium, Pseudomonas sp. EL-04J Kim, H. S.;G. T. Park;H. J. Son;S. H. Park;S. J. Lee
  8. Kor. J. Appl. Microbiol. Biotechnol. v.22 Biodegradation of trichloroethylene by a phenolutillizing bacterium Lee, S. H.;S. Y. Kang;J. H. Ha
  9. Basic Environmental Toxicology Lorris, G. C.;B. S. Shane
  10. Appl. Environ. Microbiol. v.54 Trichloroethylene metabolism by microorganisms that degrade aromatic compounds Nelson, M. J. K.;S. O. Montgomery;P. H. Pritchard
  11. Appl. Microbiol. Biotechnol. v.47 Application of the polymerase chain reaction (PCR) and reverse transcriptase/PCR for determining the fate of phenol-degrading Pseudomonas putida ATCC 11172 in a bioaugmented sequencing batch reactor Selvaratnam, S.;B. A. Schoedel;B. L. McFarland;C. F. Kulpa https://doi.org/10.1007/s002530050919
  12. Appl. Environ. Microbiol. v.54 Degradation of trichloroethylene by toluene dioxygenase in whole-cell studies with Pseudomonas putida F1 Wackett, L. P.;D. T. Gibson
  13. Appl. Environ. Microbiol. v.55 Trichloroethylene degradation by Escherichia coli containing the cloned Pseudomonas putida F1 toluene dioxygenase genes Zylstra, G. J.;L. P. Wackett;D. T. Gibson