Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Taraxacum mongolieum Hand-Mass Aqua-acupuncture Solution as the Blocking Agent of Carcinogenesis

포공영약침액의 발암과정 blocking agent로서의 활성

  • 손윤희 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구센터) ;
  • 김소연 (동국대학교 한의과대학 경혈학교실) ;
  • 임종국 (동국대학교 한의과대학 경혈학교실) ;
  • 남경수 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구센터)
  • Published : 2002.10.01
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Abstract

Taraxacunf mongofieum Hand-Mass aqua-acupuncture solution (TMAS) was prepared and investigated og, the effect on initiation of carcinogenesis. The following effe.Is as a blocking agent were measured. .(a) Indu.ction of quinone reductase, (b) Induction of glutathione S-transferase activity (c) Increase of reduced glutathione. TMAS was potent inducer of quinone reductase in Hepa Iclc7 murine hepatoma cells. Clutathione S-transferase activity was increased with TMAS. In addition glutathione levels were increased about 1.6-fold with TMAS in cultured murine hepatoma Hepa Iclc7 cells.

포공영약침액의 암억제 효과를 살펴보기 위하여 quinone reductase (QR) 생성유도를 생쥐의 간암세포인 Hepa Iclc7를 이용하여 측정한 결과, 포공영약침액을 처리한 Hepa Iclc7에서 QR생성이 유도되었다. 또한 포공영약침액은 phase II detoxification 효소인 glutathione S-transfer-ase (CST) 활성을 증가시키고 glutathione 생성도 증가시켰다 QR과 CST는 간세포에서 주로 생성되는 phase II효소로 발암물질에 의해 일어나는 돌연변이와 종양화 효과를 막아주고 발암물질을 무독화 시키는 것으로 알려져 있다. 따라서 본 실험에서 포공영약침액은 QR 효소 생성 유도, glutathione 함량 증가 및 CST 효소활성 증대등 phase II detoxification의 기작을 통한 발암과정의 blocking agent로서 효과가 있음을 알 수 있었다.

Keywords

References

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