Journal of Microbiology and Biotechnology

  • 제12권5호
  • /
  • Pages.841-843
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  • 2002
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Rapid and Simple Method to Prepare Functional Pfu DNA Polymerase Expressed in Escherichia coli Periplasm

  • Chae, Young-Kee (Department of Applied Chemistry and Recombinant Protein Expression Center (RPEC), Sejong University) ;
  • Jeon, Woo-Chun (Department of Applied Chemistry and Recombinant Protein Expression Center (RPEC), Sejong University) ;
  • Cho, Kyoung-Suk (Department of Applied Chemistry and Recombinant Protein Expression Center (RPEC), Sejong University)
  • 발행 : 2002.10.01
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초록

Pfu DNA polymerase from Pyrococcus furiosus was expressed in the E. coli periplasm, and the fully active polymerase was partially purified by applying osmotic shock, ammonium sulfate precipitation, and heat treatment. This method represents a new way of expressing and purifying functional Pfu DNA polymerase without the use of chromatography.

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참고문헌

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