Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Isolation and Cultivation Characteristics of Acetobacter xylinum KJ-1 Producing Bacterial Cellulose in Shaking Cultures

  • Son, Chang-Jin (Department of Environmental Engineering, Chonnam National University) ;
  • Chung, Seon-Yong (Department of Environmental Engineering, Chonnam National University) ;
  • Lee, Ji-Eun (Department of Environmental Engineering, Chonnam National University) ;
  • Kim, Seong-Jun (Department of Environmental Engineering, Chonnam National University)
  • Published : 2002.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

Eight strains producing bacterial cellulose (BC) were isolated from rotten fruits and traditionally fermented vinegars. One of the isolated strains from the rotten grape in Gwangju, Korea, maintained a relatively stable BC production in shaking cultures. This isolated strain proved to be Acetobacter xylinum, based on several biochemical and morphological tests. It was shown that the slant-baffled flask was more efficient than the conventional flask for the BC production in shaking cultures. To determine the most suitable carbon and nitrogen sources for the production of BC, various compounds were examined. Fructose was found to be the most effective carbon source with an optimal concentration of 2%. Mixed carbon source (glucose:fructose=1:3) was also better than glucose or fructose alone. Optimal nitrogen source, when basal medium was used, was 10% (v/v) com steep liquor (CSL). When com steep liquor was used with a mixed carbon source (glucose:fructose=1 :3),4% CSL exhibited the best BC production. Based on these results, a defined medium was developed for the BC production by Acetobacter xylinum KJ-1. When this medium was used under optimal culture conditions, the BC production was 7.2 g/1, which was approximately 3 times higher than that with the traditional HS medium.

Keywords

References

  1. J. Chem. Soc. v.49 An acetic ferment which forms cellulose Brown, A. J. https://doi.org/10.1039/ct8864900432
  2. Appl. Biochem. Biotechnol. v.24;25 Acetobacter cellulose pellicle as a tmporary skin substitute Fontana, J. D.;A. M. Souza;C. K. Fontana;I. L. Torriani;J. C. Moreschi;B. J. Gallotti;S. J. Souza;G. P. Narcisco;J. A. Bichara;L. E. X. Farah
  3. Biochem. J. v.56 Synthesis of cellulose by Acetobacter xylinum.Ⅰ. Micromethd for the determination of cellulose Hestrin, S.;M. Schramm
  4. Bergey's Manual of Dterminative Bacteriology(9th ed.) Holt, J. H.;N. R. Krieg;P. H. A. Sneath;J. T. Staley;S. T. Williams
  5. J. Mater. Sci. v.35 Bacterial cellulose - a masterpiece of nature Iguchi, M.;S. Yamanaka;A. Budhiono https://doi.org/10.1023/A:1004775229149
  6. Biosci. Biotech. Biochem. v.59 Increase in cellulose producing by sulfaguaonidineresistant mutants derived from Acetobacter xylinum subsp. sucrofermentans Ishikawa, A.;M. Matsuoka;T. Tsuchida;F. Yoshinaga https://doi.org/10.1271/bbb.59.2259
  7. Pulp & Paper v.May Bacterial cellulose has potential application as new paper coating Johnson, D. C.;A. R. Winslow
  8. J. Microbiol. Biotechnol. v.9 Modification of Acetobacter xylinum bacterial cellulose using dextransucrase and alternansucrase Kim, D. M.;Y. M. Kim;M. R. Park;D. H. Park
  9. Bergey's Manual of Systematic Bacteriology v.1 Krieg, N. R.;J. G. Holt
  10. J. Ferment. Bioeng. v.75 Production of cellulose from glucose by Acetobacter xylinum Masaoka, S.;T. Ohe;N. Sakota https://doi.org/10.1016/0922-338X(93)90171-4
  11. Biosci. Biotech. Biochem. v.60 A synthetic medium for bacterial cellulose productin by Acetobacter xylinum subsp. Sucrofermentans. Matsuoka, M. T. Tsuchida;K. Matsushita;O. Adachi;F. Yoshinage https://doi.org/10.1271/bbb.60.575
  12. J. Microbiol. Biotechnol. v.9 Effect of gluconic acid on the production of cellulose in Acetobacter xylinum BRC5 Park, S. T.;T. S. Song;Y. M. Kim
  13. Polym. Degrad. Stab. v.59 Production and application of microbial cellulose Rainer, J.;F. F. Luiz https://doi.org/10.1016/S0141-3910(97)00197-3
  14. Organic Chemistry(6th ed.) Ralph, J. F.;J. S. Fessenden;W. L. Marshall
  15. Microbial. Rev. v.55 Cellulose biosynthesis and function in bacteria Ross, P.;R. Mayer;M. Benzimanm
  16. Med. Fac. Landbouw. Univ. Gent v.59;4b Formation and application of bacterial cellulose in research, industry and medicine Schmauder, H. P.;U. Geyer;L. Einfeldt;D. Klemm;S. Marsch
  17. Biosci. Biotech. Biochem. v.59 Screening of bacterial cellulose producting Acetobacter strains suitable for agitated culture Toyosaki, H.;T. Naritomi;A. Seto;M. Matsuoka;T. Tsuchida;F. Yoshinaga https://doi.org/10.1271/bbb.59.1498
  18. J. Gen. Microbiol. v.128 Cellulose-negative mutants of Acetobacter xylinum Valla, S.;J. Kjosbakken
  19. Polym. Degrad. Stab. v.59 Improved production of bacterial cellulose and its application potential Vandamme, E. J.;S. De Baets;A. Vanbaelen;K. Joris;D. De Wulf https://doi.org/10.1016/S0141-3910(97)00185-7
  20. Methods in Enzymology. v.160 Biomass part A: cellulos and hemicellulose Wod, A. W.;S. T. Kellogg
  21. Cellulosic Polymers, Blends and Composites Applications of bacterial cellulose in cellulosic polymers Yamanaka, S.;K. Watanabe;R. Gilbert(ed.)
  22. J. Mater. Sci. v.24 The structure and mechanical properties of sheets prepared from bacterial cellulose Yamanaka, S.;K. Watanabe;N. Kitamura;M. Iguchi;S. Mitsuhashi;Y. Nishi;M. Uryr. https://doi.org/10.1007/BF01139032