Effects of Submersion Aging in Chilled Water on Lipid Oxidation, Myoglobin Oxidation and Purge Loss of Vacuum-Packed Hanwoo Meat

진공포장 한우육의 냉수 침지 숙성이 지방산화, 육색소 산화 및 포장감량에 미치는 영향

  • Published : 2002.09.01

Abstract

Efficacy of submersion aging in chilled water of vacuum-packed Hanwoo beef was investigated. At 24 hours post-mortem, the somimemebranosus portion of Hanwoo carcass was excised and sliced (2.5cm, thickness). After beef core samples(6$\textrm{cm}^2$${\times}$2cm) were prepared and vacuum packed, the samples were randomly assigned to the four treatments which were conventional refrigerator at 4$^{\circ}C$(control), conventional refrigerator at 1$^{\circ}C$(T1), submersion in chilled water at 4$^{\circ}C$(T2) and submersion in chilled water at 1$^{\circ}C$(T3). Samples were stored for 3, 7, 10 and 14 days to measure meat color(CIE L*, a*, b*), deoxymyoglobin, oxymyoglobin and metmyoglobin percentage, thiobarbituric acid reactive substance (TBARS) value, purge loss(%) and total heam pigment content. CIE a* value of samples was not significantly changed by treatment during the storage. However, metmyoglobin content of T2 samples was significantly (p<0.05) lower than those of control and T1,T3 during storage. Also, TBARS value of T3 samples was significantly (p<0.05) lower than those of control and T2 samples. T3 showed the lowest purge loss%, whereas control remarked the highest purge loss(%). Total heam pigment of all samples were not significantly changed during the storage. These results suggested that submersion aging in chilled water could keep the myoglobin stability and reduce lipid oxidation and purge loss of vacuum packed beef during storage.

진공포장한 한우육 우둔부위를 1$^{\circ}C$, 4$^{\circ}C$의 일반공기순환 냉장고와 1$^{\circ}C$, 4$^{\circ}C$의 냉수에 침지하여 3, 7, 10, 14일 동안 저장하였을 때, 처리구간의 물리적 육색 (CIE L*, a*, b*)은 크게 변화하지 않은 것으로 나타났다. 그러나 육색소 산화를 나타내는 MetMb의 함량은 처리구간에 따라 변화를 보여 대조구 (4$^{\circ}C$-공기순화)가 가장 빠른 증가를 나타내었으며, 저장 14일째 T2 (4$^{\circ}C$-냉수침지)구가 유의적으로 (p<0.05) 낮은 MetMb함량을 보였다. 한편 이런 결과는 지방산화와도 유사한 경향을 보였는데, 대조구 (4$^{\circ}C$-공기순환)의 TBARS 수치가 저장기간 동안 가장 빠른 증가를 나타낸 반면, T3(1$^{\circ}C$-냉수침지) 구의 TBARS는 크게 증가하지 않았다. 이러한 결과는 냉수침지 방법이 지방산화와 육색소 산화를 억제시킬 수 있다는 것을 의미한다. 포장감량 또는 공기순환 처리구(control, T1)가 냉수침지 처리구(T2, T3)에 비해 높게 나타났다. 총 육색소 함량은 전 저장기간 동안 처리구간에 유의적(p>0.05)인 차이가 없었다. 이상의 결과를 종합해 볼 때, 한우육을 진공포장하여 1$^{\circ}C$ 냉수에 침지시켜 숙성시키는 방법이 지방산화를 억제시키고 육색소의 안전성을 유지할 뿐만 아니라 포장감량도 줄일 수 있는 숙성법이라고 사료된다.

Keywords

References

  1. Batifoulier, F., Mercier, Y., Gatellier, P., and Renerre, M. (2002) Influence of vitamin E on lipid and protein oxidation induced by $H_2O_2$-activated MetMb in microsomal membranes from turkey muscle. Meat Sci., 61, 389-395 https://doi.org/10.1016/S0309-1740(01)00209-1
  2. Buege, J. A., and Aust, J. D. (1978) Microsomal lipid peroxidation. Methods Enzymol, 52, 302-310 https://doi.org/10.1016/S0076-6879(78)52032-6
  3. Chan, W. K. M., Faustman, D., Yin, M., and Decker, E. A. (1997) Lipid oxidation induced by oxymyoglobin and metmyoglobin with involvement of $H_2O_2$ and superoxide anion. Meat Sci., 46, 181-190 https://doi.org/10.1016/S0309-1740(97)00014-4
  4. Faustman, C., and Cassens, R .G., Schaefer, D. K., Buege, D. R.,Williams, S. N. and Sheller, K. K. (1989) Improvement of pig-ment and lipid stability in Holstein steer beef by dietary supplementation on vitamin E. J. Food Sci., 54, 858 https://doi.org/10.1111/j.1365-2621.1989.tb07899.x
  5. Forrest, J. C., Aberle, E. D., Hedrick, H. B., Judge, M. D. and Merkel, R. A. (1975) Principle of meat science, W. H. Freeman & Co.- San Francisco
  6. Homsey, H. C. (1956) The colour of cooked cured pork. l-Estimation of the nitric oxide haem pigments. J. Sci. Food Agrc., 20, 534-540
  7. Hunt, M. C., and Hedrick, H. B. (1977) Profile of fiber type and related properties of five bovine muscles. J. Food Sci., 42, 513-517 https://doi.org/10.1111/j.1365-2621.1977.tb01535.x
  8. Hur, S. J., Joo, S. T., Oh, S. H., Kim, Y. J., Kim, Y. H., Lee, J. I., and Park, G. B. (2001) Effects of packaging method and storage condition on meat shelf-life and water-holding capacity of pork loin. J. Anim. Sci. & Technol., (kor). 43(1), 121-130
  9. Husband, P. M. (1982) The history of vacuum packaged meat. Food Technology in Australia., 34(6), 272-276
  10. Joo, S. T., and Park, G. B. (2001) Instruments for meat aging. Korea Patent 0295552
  11. Kanner, J. (1994) Oxidative processes in meat and meat products: quality implication. Meat Sci., 36, 169-189 https://doi.org/10.1016/0309-1740(94)90040-X
  12. Kauffman, R. G. Carpenter, Z. L., Bray, R. W. and Hoekstra, W. G., (1964) Interrelationships ofgross chemical components of porkmuscle. J. Agr. Food Chem., 12, 102-105 https://doi.org/10.1021/jf60132a001
  13. Kauffman, R. G., Eikelenboom, G., Vander Wal, P. G., Engle, B.,and Zaar, M. (1986) A comparison of methods to estimate water-holding capacity in post-rigor porcine muscle. Meat Sci., 18, 307-311 https://doi.org/10.1016/0309-1740(86)90020-3
  14. Krzywicki. K. (1982) The determination of haem pigments in meat. Meat Sci., 1, 29-36
  15. Labadie, J. (1999) Consequences of packaging on bacterial growth meat in an ecological niche. Meat Sci., 52, 299-305 https://doi.org/10.1016/S0309-1740(99)00006-6
  16. Ledward, D. A. (1983) Haemoproteins in meat and meat products. In : Development in food proteins. III(edited by B. J. F. Hudson). pp. 33-68. London: Elsevier Applied science
  17. Liu, Q., Lanari, M. C. and Schaefer, D. M. (1995) A review of dietary vitamin E supplementation for improvement of beef quality. J. Anim. Sci., 73, 3131-3140 https://doi.org/10.2527/1995.73103131x
  18. Lynch, N. M., Kastner, C. L., Kropf, D. H. and Caul, J. F. (1986) Flavor and aroma influences on acceptance of polyvinyl chloride versus vacuum packaged ground beef. J. Food. Sci., 51, 256-257 https://doi.org/10.1111/j.1365-2621.1986.tb11103.x
  19. Newman, E. S. R., Rice-Evans, C. A. and Davies, M. J. (1991)IdentiScation of initiating agents in myoglobin-induced lipid peroxidation. Biochemical and Biophysical Research Communication., 79, 1414-1419
  20. O'keefe, M., and Hood, D. E. (1982) Biochemical factors in-Huencing metmyoglobin formation in beef from muscles of different color stability. Meat Sci., 1, 209-228
  21. Renerre, M. (1984) Variabilite entre cuscles et entre animaux de la stabilite de la couleur des viande bovines. Sciences des Aliments, 4, 567-584
  22. Renerre, M. (1999) Biochemical basis of fresh meat colour. Proc. 45th ICoMST. pp. 344-353
  23. SAS (1995) SAS/SATT software for PC. Release 6.11, SAS Institute, Cary, NC, U.S.A
  24. Taylor, A. A. (1985) Packaging fresh meat. In: developments in meat science, 3(edited by R.A. Lawrie). pp. 89-113. London : Elsevier Applied Science
  25. Warriss, P. D. (1979) The extraction of haem pigments from freshmeat. J. Food Technol., 14, 75-80 https://doi.org/10.1111/j.1365-2621.1979.tb00849.x