Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Induction of Apoptosis in Human Monocytes by Human Cytomegalovirus is Related with Calcium Increase

  • Moon, Myung-Sook (Division of Life Science, Chungbuk National University) ;
  • Lee, Gyu-Cheol (Division of Life Science, Chungbuk National University) ;
  • Lee, Chan H. (Division of Life Science, and Biotechnology Research Institute, Chungbuk National University)
  • Published : 2002.09.01
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Abstract

The effect of human cytomegalovirus (HCMV) on three human monocyte cell lines at different stages of differentiation was investigated. While the viability of HL-60 cells or U-937 cells was not significantly affected by HCMV infection, the viability of THP-1 cells was reduced. Acridine orange/ethidiurn bromide staining revealed that the reduction of THP-1 cell viability was due to increased apoptotic death following HCMV infection. Apoptosis in HL-60 cells was not affected by HCMV infection, and induction of apoptosis of U-937 cells by HCMV was intermediate between HL-60 and THP-1 cells. Since HL-60 cells are the least differentiated and THP-1 cells are the most differentiated, the induction of apoptosis of human monocytes appears to be related to the degree of cell differentiation. Flow cytometric and confocal microscopic studies using fluorescent calcium indicator Fluo-3 suggested a significant increase in intracellular free calcium concentration (［Ca$\^$2+/］i) in THP-1 cells undergoing apoptosis by HCMV infection. Again ［Ca$\^$2+/］i in HCMV-infected HL-60 cells was not critically altered, and that in HCMV-infected U-937 cells was intermediate between THP-1 cells and HL-60 cells. Calcium influx blockers such as verapamil and nifedipine partially reversed HCMV-induced apoptosis in THP-1 cells.

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References

  1. Fields Virology Cytomegalovirus Britt, W.J.;C.A. Alford;Fields, B.N.(eds.);D.M. Knipe(eds.);P.M. Howley(eds.)
  2. Ann. Rev. Biochem v.56 Intracellular calcium homeostasis Carafoli, E.
  3. Trends Microbiol v.7 Apoptosis: an innate immune response to virus infection Everett, H.;G. McFadden.
  4. Principles of Virology Flint, S.J.;L.W. Enquist;R.M. Krug;V.R. Racaniello;A.M.Skalka
  5. Trends Microbiol v.8 Exploitation of cellular regulatory pathways by human cytomegalovirus Fortunato, E.A.;A.K. McElroy;V. Sanchez;D.H. Spector
  6. Blood v.88 Direct growth suppression of myeloid bone marrow progenitor cells but not cord blood progenitors by human cytomegalovirus in vitro Holberg-Petersen, M.;H. Rollag;S. Beck;I. Overli;G. Tjonnfjord;T.G. Abrahamsen;M. Degre;K. Hestdal
  7. J. Virol v.65 Human cytomegalovirus productively infects differentiated macrophages Ibanez, C.E.;R. Schrier;P. Ghazal;C. Wiley;J.A. Nelson.
  8. Mol. Cells v.5 Effect of human cytomegalovirus immediate early gene expression on cytosolic free calcium concentration Kang, K.H.;C.H. Lee
  9. Br. J. Cancer v.26 Apoptosis: a basic biological phenomenon with wide ranging implication in tissue kinetics Kerr, J.-F.-R.;A.-H. Wyllie;A.-R. Currie
  10. Nucleic Acids Res v.19 The 21bp repeat element of the human cytomegalovirus major immediate early enhancer is a negative regulator of gene expression in undifferentiated cells Kothari, S.;J. Baillie;J.G. Sissons;J.H. Sinclair
  11. Mol. Cells v.9 Factors affecting human cytomegalovirus gene expression in human monocyte cell lines Lee, C.-H.;G.-C. Lee;Y.-J. Chan;C.-J. Chiou;J.-H. Ahn;G.S. Hayward
  12. Am. J. Med v.81 Infection in bone marrow transplant recipients Myers, J.D
  13. Curr. Top. Microbiol. Immunol v.154 Regulation and tissue-specific expression of human cytomegalovirus Nelson, J.A.;J.W. Gnann;P. Ghazal
  14. Virology v.157 Ca²+ responses in cytomegalovirus-infected fibroblasts of human origin Nokta, M.;D. Eaton;O.S. Steinsland;T. Albrecht
  15. Ann. Rev. MicrobioL v.53 Viruses and apoptosis Roulston, A.;R.C. Marcellus;P.E. Branton
  16. J. Gen. Virol v.73 Repression of human cytomegalovirus major immediate early gene expression in a monocytic cell line Sinclair, J.H.;J. Baillie;L.A. Bryant;J.A. Taylor-Wiedeman;J.G. Sissons
  17. Blood v.88 Human cytomegalovirus suppression of and latency in early hematopoietic progenitor cells Sindre, H.;G.E. Tjoonnfjord;H. Rollag;T. Ranneberg-Nilsen;O.P. Veiby;S. Beck;M. Degre;K. Hestdal
  18. APMIS v.108 Human cytomegalovirus induces apoptosis in the hematopoietic cell line MO7e Sindre, H.;H. Rollag;M.K. Olafsen;M. Degre;K. Hestdal
  19. Exp. Gerontol v.36 The programmed death phenomena, aging and the Samuri Law of Biology Skulachev, V.P.
  20. J. Virol v.68 Induction of endogenous human cytomegalovirus gene expression after differentiation of monocytes from healthy carries Taylor-Wiedeman, J.;P. Sissons;J. Sinclair
  21. Arch. Pathol. Lab. Med v.113 Posttransfusion cytomegalovirus infections Tegtmeier, G.E.
  22. Ann. Rev. Cell Biol v.6 Calcium channels: store, and oscillations Tsien, R.W.;R.Y. Tsien
  23. J. Immunol v.140 Phorbol esterinduced differentiation permits productive human cytomegalovirus infection in a monocytic cell line Weinshenker, B.G.;S. Wilton;G.P. Rice
  24. Curr. Opin. Cell Biol v.13 Ion homeostasis and apoptosis Yu, S.P.;L.M. Canzoniero;D.W. Choi