Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Characterization of Cell Wall Proteins from the soo1-1/ret1-1 Mutant of Saccharomyces cerevisiae

  • Lee, Dong-Won (Department of Microbiology, Chungnam National University) ;
  • Kim, Ki-Hyun (Department of Microbiology, Chungnam National University) ;
  • Chun, Se-Chul (Institute of Biotechnology, Chungnam National University) ;
  • Park, Hee-Moon (Department of Microbiology, Chungnam National University, Institute of Biotechnology, Chungnam National University)
  • Published : 2002.09.01
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Abstract

In order to investigate the function of Soo1p/${\alpha}$-COP during post-translational modification and intra-cellular transport of cell wall proteins in Saccharomyces cerevisiae, cell wall proteins from the soo1-1/ret1-1 mutant cells were analyzed. SDS-PAGE analysis of biotin labeled cell wall proteins suggested that the soo1-1 mutation impairs post-translational modification of cell wall proteins, such as N- and/ or Ο-glycosylation. Analysis of cell wall proteins with antibodies against ${\beta}$-1,3-glucan and ${\beta}$-1,6-glucan revealed alteration of the linkage between cell wall proteins and ${\beta}$-glucans in the soo1-1 mutant cells. Compositional sugar analysis of the cell wall proteins also suggested that the soo1-1 mutation impairs glycosylation of cell wall protein in the ER, which is crucial for the maintenance of cell wall integrity.

Keywords

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