한국가금학회지 (Korean Journal of Poultry Science)

  • 제29권3호
  • /
  • Pages.225-231
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  • 2002
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  • 1225-6625(pISSN)
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  • 2287-5387(eISSN)
한국가금학회 (The Korean Society of Poultry Science)
권호 표지

유산균과 효모의 단독 및 혼합 급여가 산란계의 생산성, 영양소 소화율, 장내미생물 변화 및 분의 암모니아 발생량에 미치는 영향

Effects of Single or Mixed Feeding of Lactobacillus and Yeast on Performance, Nutrient Digestibility, Intestinal Microflora, and Fecal NH$_{3}$ Gas Emission in Laying Hens

  • 김상호 (축산기술연구소 가금과) ;
  • 유동조 (축산기술연구소 가금과) ;
  • 박수영 (축산기술연구소 가금과) ;
  • 이상진 (축산기술연구소 가금과) ;
  • 치철환 (축산기술연구소 가금과) ;
  • 성창근 (충남대학교 식품공학과) ;
  • 류경선 (전북대학교 생체안전연구소)
  • 발행 : 2002.09.01
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초록

본 연구는 산란계에 대한 유산균과 효모의 단독 및 혼합 첨가급여 효과를 구명하기 위하여 실시하였다. 21주령된 ISA Brown 240수를 공시하여 10주 동안 사양하면서 산란 생산성, 영양소 이용성, 회장 및 맹장내 미생물 변화, 계란품질 변화 및 계분내 암모니아발생 양상을 조사하였다. 처리구는 무첨가구와 Lactobacillus crispatus avibro1(LCH), Lactobacillus vaginalis avibro2(LVH), 효모 Pichia ferinosa SKM-1(PF)을 단일 급여한 처리와 혼합 급여한 처리구(PF＋LCH, PF＋LVH)모두 6처리구로 하였다. 사료 g 당 유산균 첨가수준은 $10^{7}$ cfu 였으며, 생효모는 3$\times$$10^{6}$ cfu 이었다. 각 처리당 4반복, 반복당 10수씩 공시하여 10주간 시험을 실시한 바 얻어진 결과는 다음과 같다. 산란율은 생균제 급여구에서 대조구에 비하여 현저하게 높았으며 P<0.05), 처리구간 차이는 없었다. 평균난중도 처리구간에 통계적인 차이가 없었다. 1일 산란량은 생균제 첨가가 모두 대조구에 비하여 현저하게 개선되었으며(P<0.05), 처리구간에 차이는 나타나지 않았다. 사료섭취량은 처리간에 통계적인 차이가 없었으며, 난생산 사료효율은 생균제 첨가구가 전반적으로 개선되는 경향을 보였다. 영양소 이용성은 생균제 급여구가 건물, 조단백질, 조지방 및 조회분 소화율이 대조구에 비하여 개선되었다. 회장내 유산균 수는 LVH, PF)LClt PF＋LVH첨가구가 대조구에 비하여 현저하게 증가하였으며(P<0.05), 효모는 맹장에서 PF구가 높았으나 PF＋LVH는 가장 났았고 회장내 혐기성 미생물은 LVEt PF＋LCH, PF＋LVH가 가장 많았다. 난각질은 PF 급여구가 다른 처리구에 비하여 저하되었으며(P<0.05), Haugh unit는 처리구간에 일관성이 없었다. 계분내 암모니아 발생량은 배분후3일부터 6일까지 최대로 발생하였는데, 이 시기에 생균제 첨가구가 대조구에 비하여 발생량이 현저하게 감소되었다(P<0.05). 본 연구에서 산란계에 생효모 및 유산균의 단독 혹은 혼합 첨가는 산란율을 향상시키고 계분내 암모니아 가스 발생량을 감소시켰으며, 혼합첨가가 단독 첨가보다 향상되는 효과는 나타나지 않았다.

Total 240 of ISA Brown layers were employed in this experiment to study the effects of single or mixed feeding of Lactobacillus and yeast on the performance and intestinal microflora of laying hens. They were randomly allocated to six dietary treatments; None(Control), Pichia farinosa(PF), Lactobacillus crispatus avihen1 (LCH), Lactobacillus vaginalis avihen1(LVH), LCH＋PF, and LVH＋PF. Viable microflora were added to meet 3${\times}$10$\^$6/ cfu PF and 10$\^$7/ cfu Lacrobacillus per g of feed. There were four replicates per treatment, and 10 birds per replicates. Laying performance was recorded for 10 weeks, followed by a metabolism trial during which nutrient utilization, pattern of intestinal microflora and fecal NH$\sub$3/ emission were examined. Egg production and daily egg mass of birds fed either single or mixed microorganisms were significantly higher than those of the control(P<0.05). Egg weight and feed intake were not statistically different among all treatments. However, feed conversion ratio tended to improve by the supplementation of microbes. Digestibility of crude protein, ether extract and crude ash tended to improve in Lactobacillus treatments, however, there were not statistically different. With regards to the number of intestinal microbes, number of anaerobes were increased in microbes feeding group. Eggshell quality of PF layers was significantly poorer than those of the other treatments. No consistent trend was found in Haugh Unit among all treatments. Fecal NH$\sub$3/ gas emission was significantly lower in LVH, LVH＋PF and LCH＋PF than the other treatments(P<0.05). From the result of this experiment, it could be concluded that single or mixed feeding of Lactobacillus and yeast improves the laying performance and decreases the fecal ammonia gas emission. No synergic effect was found when both microbes were mixed and fed to the layers.

키워드

참고문헌

  1. Official methods of analysis(16th ed.) AOAC
  2. Poultry Sci v.70 The role of intestinal microflora on the prevention of Salmonella colonization in gnotobiotic chickens Baba,E.;Nagaishi,S.;Fukata,T.;Arakawa,A. https://doi.org/10.3382/ps.0701902
  3. Poultry Sci v.70 Lack of effect of a live yeast culture on broiler breeder and progeny performance Brake,J. https://doi.org/10.3382/ps.0701037
  4. Poultry Sci v.57 Interrelationship of Lactobacillus and zinc bacitracin in the diets of turkey poults Francis,C.;Janky,D.M.;Arafa,A.S.;Harms,R.H. https://doi.org/10.3382/ps.0571687
  5. J Appl Bacteriol v.36 Ecological studies on the Lactobacillus flora associated with the crop epithelium of the fowl Fuller,R. https://doi.org/10.1111/j.1365-2672.1973.tb04080.x
  6. J Appl Bacteriol. v.66 Probiotics in man and animals Fuller,R. https://doi.org/10.1111/j.1365-2672.1989.tb05105.x
  7. Poultry Sci v.18 The effect of Lactobacillus acidophilus on the proproduction of hens eggs Haddadin,M.S.Y.;Abdulrahim,S.M.;Hashilamoun,E.A.R.;Robinson,R.K.
  8. Probiotics: a general view, in the lactic acid bacteria in health and disease v.1 Havenaar,R.;Huis in't Veld JHJ;J.B.J.Wood(ed.)
  9. J Exp Zool v.3 no.suppl. Ammonia production from uric acid, urea, and amino acids and its absorption from the ceca of the cockerel Karasawa,Y.
  10. Dairymen's Digest December 20 Feed additive helps earth producers Mason,T.R.
  11. Hoard's Dairyman v.125 How to feed for 20,000 pounds of milk McCullough,M.E.
  12. British Journal of Nutrition v.76 Effects of a mixture of organisms, Lactobacillus acidophilus or Streptococcus faecalis on cholesterol metabolism in rats fed on a fat- and cholesterol-enriched diet Michihiro,F.;Nakano,M. https://doi.org/10.1079/BJN19960092
  13. Poultry Sci v.73 Performance of Single Comb White Leghorn layers fed corn-soybean meal and barley-com-soybean meal diets supplemented with a direct fed microbial Nahashon,S.N.;Nakaue,H.S.;Snyder,S.P.;Mirosh,L.W. https://doi.org/10.3382/ps.0731712
  14. Proceedings of alltech's eleventh annual symposium Using yeast culture and lactic acid bacteria in broiler breeder diets. Biotechnology in the feed industry Reynaldo,G.M.
  15. Poultry Sci v.28 Nutritive properties of torula yeast for poultry Ringrose,R.C. https://doi.org/10.3382/ps.0280075
  16. Biology and Activities of Yeasts. The society for Applied Bacteriology Symposium Series no.9 Recent research on industrially important atrains of Saccharomyces cerevisiae Rose,A.H.;Skinner,F.A.(ed.);S.M.Passmore(ed.);P.P.Davenport(ed.)
  17. SAS/STAT？ User's Guide Release 6.12 Edition SAS Institute
  18. Probiotics: Theory and Applications Stark,B.A.;Wilkinson,J.M.
  19. Poultry Sci v.44 Fatty acid digestibility in laying hens fed yeast culture Tonkinson,L.V.;Gleaves,E.W.;Dunkelgod,K.E.;Thayer,R.H.;Sirny,R.J.;Morrison,R.D. https://doi.org/10.3382/ps.0440159
  20. Poultry Sci v.52 Influence of the implantation of Lactobacillus acidophilus in chicks on the growth, feed conversion, malabsorption of fats syndrome and intestinal flora Tortuero,F. https://doi.org/10.3382/ps.0520197
  21. Anim Feed Technol v.53 Effects of inclusion of microbial cultures in barley-based diets fed to laying hens Tortuero,F.;Fernandez,E. https://doi.org/10.1016/0377-8401(94)00747-W
  22. Avian Dis v.29 Further studies on competitive exclusion of Salmonella typhimurium by lactobacilli in chickens Weinack,O.M.;Snoeyenbos,G.H.;Soerjadi-Liem,A.S. https://doi.org/10.2307/1590485
  23. Poultry Sci v.76 Effect of feeding diets containing an antibiotic, a probiotic, or yucca extract on growth and intestinal urease activity in broiler chicks Yeo,J.;Kim,K.I. https://doi.org/10.1093/ps/76.2.381
  24. 한국가금학회지 v.27 유산균의 첨가 급여가 산란 생산성, 소화기관 미생물 변화 및 계란 품질에 미치는 영향 김상호;박수영;유동조;이상진;강보석;최철환;류경선
  25. 한국가금학회지 v.28 no.1 유산균과 버지니아마이신의 급여가 육계의 생산성 및 장내미생물에 미치는 영향 김상호;박수영;유동조;이상진;류경선
  26. 한국영양사료학회지 v.18 사료내 생산성 개선제의 첨가가 산란계의 생산성과 에너지 이용효율에 미치는 영향 류연선;한인규;김성우
  27. 한국가금학회지 v.28 no.1 유산균의 급여가 육계의 성장능력에 미치는 영향 박수영;김상호;유동조;이상진;류경선
  28. 한국가금학회지 v.22 생효모 배양물의 급여가 산란계의 생산성에 미치는 영향 이을연;이봉덕;지설하;박홍석