Preventive Nutrition and Food Science

  • 제7권3호
  • /
  • Pages.310-316
  • /
  • 2002
  • /
  • 2287-1098(pISSN)
  • /
  • 2287-8602(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
권호 표지
DOI QR코드

DOI QR Code

Purification and Characterization of Cyclodextrin Glucanotransferase from Paenibacillus sp. JK-12

  • Kang, Yong (Division of Biological Sciences, Pusan National University) ;
  • Kim, Sung-Koo (Department of Biotechnology and Bioengineering, Pukyung National University) ;
  • Jun, Hong-Ki (Division of Biological Sciences, Pusan National University)
  • 발행 : 2002.09.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Extracellular cyclodextrin glucanotransferase (CGTase) from Paenibacillus sp. JK-12 was purified through sev-eral purification steps consisting of ammonium sulfate precipitation and chromatographies on DEAE-sephadex A-50 and Mono QIM HR5/5. The purified CGTase exhibited a single band on SDS-PAGE and was estimated to be approximately 82 kDa. The isoelectric point of the enzyme was 7.2 as determined by isoelectric focusing. The CGTase from Paenibacillus sp. JK-12 had a transglucosylation activity at the C-2 position of L-ascorbic acid. The optimum pH and temperature for the CGTase activity were 8.0 and 5$0^{\circ}C$, respectively. The enzyme activity was stable from pH 6.0 to 9.() and at temperatures up to 55$^{\circ}C$ at pB 8.0, having 80% residual activity. The activity of the CGTase was strongly resistant to metals such as A $g^{+}$ and $Ba^{2+}$ but slightly inhibited by H $g^{+}$, N $i^{2+}$ and $Mg^{2+}$. The enzymeproduced $\alpha$ -cyclodextrin ($\alpha$-CD) and $\beta$-CD as the main products from starch, but not ${\gamma}$-CD.X>-CD.

키워드

참고문헌

  1. Burns JJ, Rives JM, Machin LJ. 1987. Third conference on vitamin C. Ann NY Acad Sci 498: 1-533 https://doi.org/10.1111/j.1749-6632.1987.tb23747.x
  2. Tajima S, Pinnell RS. 1982. Regulation of collagen synthesis by ascorbic acid, ascorbic acid increases type I procollagen mRNA. Biochem Biophys Res Commun 106: 632-637 https://doi.org/10.1016/0006-291X(82)91157-3
  3. Tolbert BM, Downing M, Carlson RW, Knight MK, Baker EM. 1975. Chemistry and metabolism of ascorbic acid and ascorbate sulfate. Ann NY Acad Sci 258: 48-69 https://doi.org/10.1111/j.1749-6632.1975.tb29267.x
  4. Yamamoto I, Muto N, Murakami K, Suga S, Yamaguchi H. 1990. L-ascorbic acid $\alpha$-glucoside formed by regioselective transglycosylation with rat intestinal and rice seed $\alpha$-glucosidases: Its improved stability and structure determination, Chem Pharm Bull 38: 3020-3023 https://doi.org/10.1248/cpb.38.3020
  5. Tanaka M, Muto N, Yamamoto I. 1991. Characterization of Bacillus stearothermophilus cyclodextrin glucanotransferase in ascorbic acid 2-O-$\alpha$-glucoside formation. Biochem Biophys Acta 1078: 127-132 https://doi.org/10.1016/0167-4838(91)99000-1
  6. Muto N, Nakamura T, Yamamoto I. 1990. Enzymatic formation of a nonreducing L-ascorbic acid $\alpha$-glucoside: purification and properties of $\alpha$-glucosidase catalyzing site- specific transglucosylation from rat small intestine. J Biochem 107: 222-227 https://doi.org/10.1093/oxfordjournals.jbchem.a123030
  7. Yamamoto I, Muto N, Nagata E, Nakamura T, Suzuki Y. 1990. Formation of a stable L-ascorbic acid $\alpha$-glucoside by mammalian $\alpha$-glucosidase-catalyzed site-specific transglucosylation. Biochem Biophys Acta 1035: 44-50 https://doi.org/10.1016/0304-4165(90)90171-R
  8. Muto N, Terasawa K, Yamamoto I. 1992. Evaluation of ascorbic acid 2-O-$\alpha$-glucoside as vitamin C source: mode of intestinal hydrolysis and absorption following oral administration. Internat J Vit Nutr Res 62: 318-323
  9. Yamamoto I, Suga S, Mitoch Y, Tanaka M, Muto N. 1990. Antiscorbutic activity of L-ascorbic acid 2-glucoside and its availability as a vitamin C supplement in normal rats and guinea pigs. J Pharmacobio-dyn 13: 688-695 https://doi.org/10.1248/bpb1978.13.688
  10. Kumano Y, Sakamoto T, Egawa M, Tanaka M, Yamamoto I. 1998. Enhancing effect of 2-O-$\alpha$-D-glucopyranosyl L- ascorbic acid, a stable ascorbic acid derivative, on collagen synthesis. Biol Pharm Bull 21: 662-666 https://doi.org/10.1248/bpb.21.662
  11. Yamamoto I, Muto N, Murakami K, Akiyama J. 1992. Collagen synthesis in human skin fibroblasts is stimulated by a stable form of ascorbate, 2-$\alpha$-D-glucopyranosyl-L-ascorbic acid. J Nutr 122: 871-877
  12. Aga H, Yoneyama M, Sakai S, Yamamoto I. 1991. Synthesis of 2-O-$\alpha$-D-glucopyranosyl L-scorbic acid by cyclomaltodextrin glucanotransferase from Bacillus stearothermophilus. Agric Biol Chem 55: 1751-1756 https://doi.org/10.1271/bbb1961.55.1751
  13. Kim TK, Park DC, Lee YH. 1997. Synthesis of glucosyl- sugar alcohols using glycosyltransferases and structural identification of glucosyl-manitol. J Appl Microbiol Biotechnol 7: 310-317
  14. Bae KM, Kim SK, Kong IS, Jun HK. 2001. Purification and properties of cyclodextrin glucanotransferase synthesizing 2-O-$\alpha$-D-glucopyranosyl L-ascorbic acid from Paenibacillus sp. JB-13. J Microbiol Biotechnol 11: 242-250
  15. Kim C, Shin HD, Lee YH. 1995 Selection of the constitutive mutant of Bacillus firmus var. alkalophilus and its characteristics of cyclodextrin glucanotransferase production. J Appl Microbiol Biotechnol 5: 61-67
  16. Park TH, Shin HD, Lee YH. 1999. Characterization of the $\beta$-cyclodextrin glycosyltransferase gene of Bacillus firmus var. alkalophilus and its expression in E. coli. J Appl Microbiol Biotechnol 9: 811-819
  17. Yu JH, Chung YJ, Lee JS. 1989. Isolation and characterization of cyclodextrin glycosyltransferase producing alkalophilic Bacillus sp. Kor J Appl Microbiol Bioeng 17: 148- 153
  18. Nelson N. 1952. A photometric adaptation of the Somogyi method for the determination of glucose. J Biol Chem 163: 401-406
  19. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protien-dye binding. Anal Biochem 72: 248-254 https://doi.org/10.1016/0003-2697(76)90527-3
  20. Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685 https://doi.org/10.1038/227680a0
  21. Jun HK, Bae KM, Kim YH. 1998. Identification of L-ascorbic acid 2-O-$\alpha$-glucoside, a stable form of ascorbic acid, in kimchi. J Appl Microbiol Biotechnol 8: 710-713
  22. Hyun D, Kim SC, Lee YH. 1998. Purification and characterization of $\beta$-cyclodextrin glucanotransferase excreted by Bacillus firmus var. alkalophilus. Kor J Appl Microbiol Biotechnol 26: 323-330
  23. Mori S, Hirose S, Oya T, Kitahata S. 1994. Purification and properties of cyclodextrin glucanotransferase from Brevibacterium sp. No. 9605. Biosci Biotech Biochem 58: 1968- 1972 https://doi.org/10.1271/bbb.58.1968
  24. Larsen, KL, Lene DO, Hans JC, Flemming M, Lars HP, Wolfgang Z. 1998. Purification and characterization of cyclodextrin glycosyltransferase from Paenibacillus sp. F8. Carbohydr Res 310: 211-219 https://doi.org/10.1016/S0008-6215(98)00178-5
  25. Kitahata S, Okada S. 1982. Comparison of action of cyclodextrin glucanotransferase from Bacillus megaterium, B. circulans, B. stearothermophilus and B. macerans. J Jpn Soc Starch Sci 29: 13-18 https://doi.org/10.5458/jag1972.29.13
  26. Yagi Y, Sato M, Ishikura T. 1986. Comparative studies of CGTases from Bacillus ohbensis, Bacillus macerans and Bacillus circulans and production of cyclodextrin using those CGTases. J Jpn Soc Starch Sci 33: 144-151 https://doi.org/10.5458/jag1972.33.144
  27. Akimaru K, Toshiharu Y, Shinpe Y. 1991. Purification and properties of Bacillus coagulans cyclodextrin glucanotransferase. J Ferment Bioeng 71: 322-328 https://doi.org/10.1016/0922-338X(91)90344-G
  28. Kitahata S, Okada S. 1982. Purification and some properties of cyclodextrin glucanotransferase from Bacillus stearothermophilus TC-60. J Jpn Soc Starch Sci 29: 7-12 https://doi.org/10.5458/jag1972.29.7
  29. Kato T, Horikoshi K. 1966. A new γ-cyclodextrin forming enzyme produced by Bacillus subtilis no. 313. J Jpn Soc Starch Sci 33: 137-143
  30. Fujita Y, Tsubouchi H, Inagi Y, Tomita K, Ozaki A, Nakanishi K. 1990. Purification and properties of cyclodextrin glycosyltransferase from Bacillus sp. AL-6. J Ferment Bioeng 70: 150-154 https://doi.org/10.1016/0922-338X(90)90174-U
  31. Wind RD, Liebl W, Buitelaar RM, Penninga D, Spreinat A, Dijkhuizeu I, Bahl H. 1995. Cyclodextrin formation by the thermostable $\alpha$-amylase of Thermoanaerobacterium thermosulfurigenes EM1 and reclassification of the enzyme as a cyclodextrin glycosyltransferase. Appl Environ Microbiol 61: 1257-1265
  32. Sin KA, Nakamura A, Masaki H, Matsuura Y, Uozumi T. 1994. Replacement of an amino acid residue of cyclodextrin glucanotransferase of Bacillus ohbensis doubles the production of γ-cyclodextrin. J Biotechnol 32: 283-288 https://doi.org/10.1016/0168-1656(94)90214-3
  33. Hill DE, Aldape R, Rozzell JD. 1990. Nucleotide sequence of a cyclodextrin glucosyltransferase gene, cgtA, from Bacillus licheniformis. Nucl Acid Res 18: 199-204 https://doi.org/10.1093/nar/18.1.199
  34. Nitschke L, Heeger K, Bender H, Schulz GE. 1990. Molecular cloning, nucleotide sequence and expression in Escherichia coli of the $\beta$-cyclodextrin glycosyltransferase gene from Bacillus circulans strain no. 8. Appl Microbiol Biotechnol 33: 542-546
  35. Tonkova A. 1998. Bacterial cyclodextrin glucanotransferase. Enzyme and Microbial Technology 22: 678-686 https://doi.org/10.1016/S0141-0229(97)00263-9
  36. Nakamura N, Horikoshi K. 1976. Characterization and some cultural conditions of a cyclodextrin glycosyltransferase- producing alkalophilic Bacillus sp. Agric Biol Chem 40: 753- 757 https://doi.org/10.1271/bbb1961.40.753
  37. Nakamura N, Horikoshi K. 1976. Purification and properties of cyclodextrin glycosyltransferase of an alkalophilic Bacillus sp. Agric Biol Chem 40: 935-941 https://doi.org/10.1271/bbb1961.40.935
  38. Shin HD, Kim C, Lee YH. 1999. The roles of tryptophan and histidine residues in the catalytic activities of $\beta$- cyclodextrin glucanotransferase from Bacillus firmus var. alkalophilus. J Appl Microbiol Biotechnol 9: 62-69
  39. Muto N, Suga S, Fujl K, Yammoto I. 1990. Formation of a stable ascorbic acid 2-glucoside by specific transglucosylation with rice seed $\alpha$-glucoside. Agric Biol Chem 54: 1697- 1703 https://doi.org/10.1271/bbb1961.54.1697

피인용 문헌

  1. -ascorbic acid with cyclodextrin glycosyltransferase vol.39, pp.2, 2019, https://doi.org/10.1080/07388551.2018.1531823