KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Antimicrobial Activity and Food Storage of LDPE Silica Film Containing Antimicrodial Compounds

항균성물질이 함유된 Silica LDPE필름의 항균효과 및 식품 저장성

  • 김현수 (계명대학교 자연과학대학 미생물학과) ;
  • 성림식 (계명대학교 자연과학대학 미생물학과) ;
  • 이인선 (계명대학교 자연과학대학 식품가공학과)
  • Published : 2002.08.01
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Abstract

Low density polyethylene(LDPE) film was fabricated with the addition of benzoic acid, or/and JP which contained water-soluble silica adsorbed a natural antimicrobial compounds. The used antimicrobial compounds were isolated from culture broths of methylotropic actinomycetes strains MO-16 and MO-17 which was newly isolated from soils. An antimicrobial compound retained antimicrobial activity after heat treatment at 121$^{\circ}C$ for 5 min. JP, which obtained from Japan as a antimicrobial agent, showed antimicrobial activity in the concentration of 100 mg/mL. The silica LDPE film revealed the growth inhibition of total aerobic bacteria in packaged minced pork compared with commercial film and of E. coil on a contained agar plate. In the storage testing of various packaged foods at room temperature for 30 days, the film showed excellent preservation compared with commercial film in case of small tomato and Agaricus bisporus.

다양한 식품의 미생물에 의한 변패를 억제하고 저장성을 높일수 있는 포장필름을 개발하기 위하여, 미생물이 생산하는 천연 항균성 물질을 흡착시킨 silica에 항균제로서 benzoic acid 및 일본산 항균성 필름 첨가제인 JP를 공동 첨가하여 항균성 LDPE 필름을 제조하였다. 천연 항균제로는 methanol 자화 방선균 MO-16과 MO-17이 생산한 항균제를 사용하였으며, 이 물질은 121$^{\circ}C$에서 5분간 열처리시에도 항균력이 유지되는 내열성이 확인되었다. 제조한 silica LDPE 필름의 미생물생육 억제효과를 검토한 결과 조분쇄한 돈육의 경우 포장하여 실온 및 4$^{\circ}C$에서 보존시 시판필름에 비해 미생물생육억제효과가 우수하였다. 고체배지에 제조필름을 첨가하여 E. coli 에 대한 항균효과를 검토한 결과 첨가량에 따라 항균효과가 우수한 것이 입증되었다. 제조필름으로 포장한 4종류의 식품에 대한 저장성을 검토한 결과 시판필름에 비해 저장성이 우수한 것이 확인되었으며 특히 양송이 및 토마토에 대한저장효과가 우수하였다.

Keywords

References

  1. Hotchkiss, J. H (1995), Safety Considerations in Active Packaging. In Active Food Packaging. M. L. Rooney, Ed, p238, Blackie Academic & Professional, London, England
  2. Hale, P. W., W. R. Miller, and J. J. Smoot (1986), Evolution of a Heat-shrinkable Copolymer Film Coated with Imazalil for Decay Control of Florida Grapefruit. Trop. Sci. 26, 67-71
  3. Weng, Y. -M. and J. H. Hotchkiss (1992), Inhibition of Surface Molds on Cheese by Polyethylene Film containing the Antimycotic Imazalil. J. Food Protect. 9,29-37.
  4. Halek, G. W. and A. Garg (1989), Fungal Inhibition by a Fungicide Coupled to an Ionomeric Film. J. Food Safety 9, 215-222 https://doi.org/10.1111/j.1745-4565.1988.tb00522.x
  5. Flanklin, N. F. and D. K Cooksey (2001), Inhibition of Listeria monocytogenes using nisin-containing packaging film., Proc. Institute of Food Technologists Annual Meeting, June, 2001, New Orleans, Louisiana, USA
  6. Weng, Y. -M. (1992), Development and Application of Food Packaging Films containing Antimicrobial Agents. Ph.D. Dissertation, Cornell University, Ithaca, NY
  7. Weng, Y. -M. and J. H. Hotchkiss (1993), Anhydrides as antimycotic agents added to polyethylene films for food packaging. Pack. Technol. Sci. 6, 123-128 https://doi.org/10.1002/pts.2770060304
  8. Koichiro, Y. (1993), Maintenance of Freshness by Antimicrobial Packaging Material using a Component of Horseradish. Food Sci. 35, 102-107
  9. Ouattara, B., R. E. Simard, G. Piette, A. Beigin, and R. A. Holley (2000), Inhibition of Surface Spoilage Bacteria in Processed Meats by Application of AantimicrobialFilms Prepared with Chitosan. Inter. J. Food Microbiol. 62, 139-148 https://doi.org/10.1016/S0168-1605(00)00407-4
  10. Begin, A. and M. -R. Van Calsteren (1999), Antimicrobial films produced from chitosan. Inter. J. Microbiol. Macromol. 26, 63-67 https://doi.org/10.1016/S0141-8130(99)00064-1
  11. Kim, K S., H. S. Sun, K W. Bae, and C. Y. Park (1997), Disinfecting Effect and Growth Enhancement of Silver coated Ceramic powder in Vegetable. Kor. J. Biotechnol. Bioeng. 12, 35-39
  12. Kim, K. S., Y. K. Kang, J. D. Kim, J. B. Eun, and C. H. Park (1998), Storage of Kimchi in LDPE Film containing Antibiotic Ceramic. Kor. J. Food Sci. Technol. 30,811-816
  13. An, D. S., Y. I. Hwang, S. H. Cho, and D. S. Lee (1998), Packaging of Fresh Curled Lettuce and Cucumber by using Low Density Polyethylene Films Impregnated with Antimicrobial Agents. J. Kor. Soc. Food Sci. Nur. 27, 675-681
  14. Chung, S. K, S. H. Cho, and D. S. Lee (1998), Modified Atmosphere Packaging of Fresh Strawberries by Antimicrobial Plastic Films. Kor. J. Food Sci. Technol. 30, 1140-1145
  15. Kim, H. S., L. S. Seong, and T. S. Yu (2000), Adsorption and Thermostability of Antimicrobial Agents on Synthetic Ceramic Power. Kor. J. Biotechnol. Bioeng. 15, 594-599
  16. Kim, H. S., L. S Seong., and T. S. Yu (2000), Antimicrobial Activity and Food Storage of LDPE Ceramic Film Containing Antimicrobial Agents. Kor. J.Biotechnol. Bioeng. 15, 600-604
  17. Kim, H. S. and J. S. Lee (1999), Purification and Production Conditions of Antimicrobial Compound from Methylotrophic Actinomycetes MO-16. kor. J. Appl. Microbiol. Biotechnol. 27(5), 391-398
  18. Kim, H. S., L. S. Seong, K. H. Lee, Y. J. Lee, I. S. Lee, and T. S. Yu (2002), Adsorption and Thermostability of Antimicrobial Agents on water-soluble Silica. Kor. J. Biotechnol. Bioeng. (in print)