Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Prediction of Continuous Reactors Performance Based on Batch Reactor Deactivation Kinetics Data of Immobilized Lipase

  • Murty, V.Ramachandra (Department of chemical Engineering, Manipal Institute of Technology, A Constituent Institution of Manipal Acad-emy of Higher Education) ;
  • Bhat, Jayadev (Department of chemical Engineering, Manipal Institute of Technology, A Constituent Institution of Manipal Acad-emy of Higher Education) ;
  • Muniswaran, P.K.A. (Department of chemical Engineering, Manipal Institute of Technology, A Constituent Institution of Manipal Acad-emy of Higher Education)
  • Published : 2002.07.01
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Abstract

Experiments on deactivation kinetics of immobilized lipase enzyme from Candida cyl-indracea were performed in stirred bath reactor using rice bran oil as the substrate and temperature as the deactivation parameter. The data were fitted In first order deactivation model. The effect of temperature on deactivation rate was represented by Arrhenius equation. Theoretical equations were developed based on pseudo-steady state approximation and Michaelis -Menten rate expression to predict the time course of conversion due to enzyme deactivation and apparent half-life of the immobilized enzyme activity in PFR and CSTH under constant feed rate polity for no diffusion limitation and diffusion limitation of first order. Stability of enzyme in these continuous reactors was predicted and factors affecting the stability were analyzed.

Keywords

References

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