Korean Journal of Animal Reproduction (한국가축번식학회지)

The Korean Society of Animal Reproduction (한국동물번식학회)
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Effect of Extender, Preservation Temperature and Period of liquid Boar Semen on Semen Characteristics and Reproductive Performance

돼지 액상정액의 보존액, 보존온도 및 기간이 정액성상과 번식성적에 미치는 영향

  • Published : 2002.03.01
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Abstract

This study was carried out to investigate the effects of extenders such as Beltsville thawing solution(BTS), Modena and Androhep, preservation temperature and period of liquid boar semen on semen characteristics and reproductive performance. Boars were raised at Swine Artificial Insemination Center in National Livestock Research Institute, Sunghwan, Chungnam, Korea. This experiment was carried out from 1995 to 2000. The results obtained were summarized as follows. 1. Sperm motility in the samples with Androhep and BTS reduced from day 5 and in the samples with Modena reduced from day 3 of storage. pH or 3 extenders varied from 6.24 to 7.04 during day 1 to 5 of storage. Farrowing rate of sows inseminated with liquid boar semen offended with BTs, Modena and Androhep extenders did not show any differences until day f after semen collection. Sows inseminated with Androhep extender had better farrowing rates (P＜0.05) than those with Modena extender at day 1 or 5 after semen collection, but farrowing rates after AI using BTS did not differ compared to those Androhep and Modena. Litter size did not show any differences among the three extenders, but Androhep had the decreased litter size from day i of storage. 2. Motility and normal acrosome of the sperm preserved at 5$^{\circ}C$ did not show any differences until day 4 of storage, but those at 17$^{\circ}C$ changed from day 3 and 4, respectively. Farrowing rate of sows artificially inseminated with liquid boa. semen preserved at 17$^{\circ}C$ had higher, than at 5$^{\circ}C$ (p＜0.05), but there was no significant differences in litter size. Farrowing rates and litter size were decreased from day 2 and day 3 of storage at 17$^{\circ}C$, respectively. Farrowing rate of sows inseminated with the preserved semen at 5$^{\circ}C$ did not changed until day 4, but the litter size at 5$^{\circ}C$ was lower than that at 17$^{\circ}C$.

돼지 인공수정센터에서 사육중인 인공수정용 종모돈을 이용하여 1995년부터 2000년까지 보존액 종류, 보존온도 및 보존기간에 따른 정액성상 변화와 번식성적을 조사하여 돼지 인공수정시 번식성적 향상과 실용화에 기여코자 본 연구를 실시하였다. 1. 돼지 액상정액의 보존액 종류에 따른 보존기간별 활력은 Androhep 보존액은 3일째부터, BTS 및 Modena는 5일째부터 현저하게 감소하는 경향을 보였고 (P＜0.05), pH변화는 6.24~7.06 범위에서 변이가 심하게 관찰되었으며 Androhep 보존액이 산도가 낮은 경향을 보였으나 전반적으로 규칙 적 인 경향을 나타내었다. 보존액 종류별 보존기간에 따른 분만율은 BTS, Modena 및 Androhep 보존액 모두 5일 보존까지 차이가 없었다. 보존액별 분만율은 Androhep 보존액이 BTS 보존액과는 차이가 없었으나 1일 보존과 5일 보존에서 Modena보존액 보다 우수하였다 (P＜0.05). 산자수는 보존 기간별 보존액간에 차이는 없었으나 Androhep 보존액은 3일 보존부터 산자수가 유의적으로 감소하였다 (P＜0.05). 2. 보존온도에 따른 액상정액의 운동성과 정상첨체 비율에서 17$^{\circ}C$ 보존정액의 운동성은 3일째부터, 정상 첨체율은 4일째부터 감소하였으나 5$^{\circ}C$ 보존정액은 4일 보존까지 정액성상의 변화가 크지 않은 것으로 나타났다. 17$^{\circ}C$에 보존한 액상정액이 5$^{\circ}C$에 보존한 것보다 분만율은 현저히 높았으나 산자수는 비슷한 경향을 보였고, 분만율은 보존 2일째부터 산자수는 3일째부터 감소하였다. 5$^{\circ}C$ 보존정액은 4일까지 분만율에 큰 변화가 없었으나 산자수는 다소 감소하는 경향을 보였다.

Keywords

References

  1. Althouse, G. C., Wilson, M. E., Kuster, C. and Parsley, M. 1998. Characterization of lower tem-perature storage limitations of fresh-extended porcine semen. Theriogenology 50:535-543
  2. Cheon, Y. M., Park, C. S., Seo, K. W. and Lee, K. S. 1996. Study on the preservation of liquid boar semen with BF5 and $B{\"{u}}tschwiler$ Diluents. Korean J. Emb. Trans. Vol. II, NO.2 pp.I59-I66
  3. Donald, G. L. 2000. Liquid boar semen production: Current extender technology and where do we go from here!. 4th Int. Conf. Boar semen preservation, pp.121-135
  4. Gottardi, L., Brunel, L. and Zanelli, L. 1980. New dilution media for artificial insemination in the pig. 9th Int. Congr. Anim. Reprod. and A. I., Madrid, Spain, Vol. V, pp.49-53
  5. Johnson, L. A., Aalbers, J. G. and Grooten, H. J. G. 1988. Artificial insemination of swine : Fecundity of boar semen stored in Beltsville TS (BTS), Modified Modena (MM), or MR-A and inseminated on one, three and four days after collection. Zuchthygiene 23:49-55
  6. Johnson, L. A., Weitze, K. F., Fiser, P. and Maxwell, W. M. C. 2000. Storage of boar semen. Anim. Reprod. Sci. 62:143-172 https://doi.org/10.1016/S0378-4320(00)00157-3
  7. Korniewicz, D., Szczesniak-Fabianczyk, B. and Smorage, Z. 1995. The survival rate and fertil-izing capacity of boar semen diluted with dif-ferent diluents. 3rd Int. Conf. Boar semen pre-servation, pp.273-274
  8. Kuster, C. E. and Althouse, G. C. 1999. The fecundity of porcine semen stored for 2 to 6 days in $Androhep^{\circledR}$ and X-cellTM extenders. Theriogenology 52:365-376 https://doi.org/10.1016/S0093-691X(99)00135-1
  9. Laforest, J. P. and Allard, D. 1995. Comparison of four extenders for long-term storage of fresh boar semen. 3rd Int. Conf. Boar semen preservation, pp: 275-276
  10. Landsverk, K. 2000. Packaging and distribution-Their impact on fertility. 4th Int. Conf. Boar semen preservation, pp: 137-139
  11. Moretti. 1981. Cited by Johnson, L. A., and Aalbers, J. G. 1984. Artificial insemination of swine: fertility using several liquid semen dil-uents. Proceedings of 8th Int. Pig Vet. Soc. Congr., Ghent, Belgium. p. 293
  12. Park, C. S., Cheon, Y. M. and Xu, Z. 1996. Comparison of preservation of liquid boar semen between Lactose-Egg Yolk and $B{\"{u}}tsch$-wiler Diluents. Korean J. Anim. Reprod, 20(2): 101-109
  13. Paulenz. H., Kommisrud, E. and Hofmo, P. O. 2000. Effect of long-term storage at different temperatures on the quality of liquid boar se-men. Reprod. Dom. Anim. 35:83-87 https://doi.org/10.1046/j.1439-0531.2000.00207.x
  14. Pursel, V. G. and Johnson, L. A. 1974. Glu-taraldehyde fixation of boar spematozoa for ac-rosome evaluation. Theriogenology 1(2):36-68
  15. Pursel, V. G. and Johnson, L. A. 1975. Freez-ing of boar spermatozoa: Fertilizing capacity with concentrated semen and a new thawing procedure, J. Anim. Sci. 40:99-102 https://doi.org/10.1126/science.40.1020.99
  16. Reed, H. C. B. 1990. Commercial requirements for an effective fresh semen diluent. 2nd Int. Conf. Boar semen preservation, pp: 255-270
  17. SAS Institute. 1988. SAS User's Guide : Sta-tistics (version 6.03), SAS Inst., Inc., Cary, NC., USA
  18. Soede, N. M., Wetzels, C. C. H., Zondag, W., de Koning, M. A. I. and Kemp, B. 1995. Effect of time of insemination relative to ovulation, as determined by ultrasonography, on fertilization rate and accessory sperm count in sows. J. Reprod. Fert. 104: 99-106 https://doi.org/10.1530/jrf.0.1040099
  19. Waberski, D. 1988. In vitro-und Besamung-sversuche unter Praxisbedingungen mit lang-zeitkonserviertem Eberflussigsperma unter be-sonderer Berucksichtigung von BSA und Puffer im Verdunnermedium. Thesis, Tierarztl. Hoch-sch. Hannover
  20. Waberski. D., Meding, S., Dirksaen, G., Weitze, K. F., Lewiding, C. and Hahn, R. 1994a. Fer-tility of long term-stored boar semen: influence of extender (Androhep and Kiev), storage time and plasma droplets in the semen. Anim. Reprod. Sci. 36:145-151
  21. Waberski, D., Weitze, K. F., Lietmann, C., Lubbert zur Lage, W., Bortolozzo, F., Willmen, T. and Petzoldt, R. 1994b. The initial fertilizing capacity of long term-stored liquid boar semen following per-and postovulatory insemination. Theriogenology 41: 1367-1377
  22. Weitze, K. F. 1990. The use of long-term extender in pig AI-a view of the international situation. Pig News Information. ll(l ):23-26
  23. Weitze, K. F., Habeck, 0., Willmen, T. and Rath, D. 1989. Detection of ovulation in the sow using transcutaneous sonography. Zuchthygiene 24:40-42
  24. 朴昌植，金敏奎，李成鎬，李千軍，李義海. 1997. 調節되지 않은 室溫에서의 돼지 液狀精液 保存에 關한 硏究. 韓國家畜繁殖學會 21(1):25-30
  25. 鄭￥基，金學奎，高文石，金仁哲，崔震成， 李光源，孫東秀， 金鉉，持卨夏，朴昌植. 1989. 5 ml 스트로에 保存한 돼지 液狀精液의 生存性과 受精能力에 關한 硏究. 韓畜誌，31(3):158-161