한국식품위생안전성학회지 (Journal of Food Hygiene and Safety)

  • 제17권2호
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  • Pages.71-78
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  • 2002
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  • 1229-1153(pISSN)
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  • 2465-9223(eISSN)
한국식품위생안전성학회 (The Korean Society of Food Hygiene and Safety)
권호 표지

백련초 (Opuntia ficus-indica var., saboten)의 Salmonella와 Escherichia coli O157 : H7에 대한 항균효과

Antimicrobial Activity of Natural Product Made by Opuntia ficus-indica var. saboten Against Salmonella spp. and Escherichia coli O157:H7

  • 김소현 (서울대학교 수의과대학 및 농생명공학 사업단 미생물학교실) ;
  • 권남훈 (서울대학교 수의과대학 및 농생명공학 사업단 미생물학교실) ;
  • 김지연 (서울대학교 수의과대학 및 농생명공학 사업단 미생물학교실) ;
  • 임지연 (서울대학교 수의과대학 및 농생명공학 사업단 미생물학교실) ;
  • 배원기 (서울대학교 수의과대학 및 농생명공학 사업단 미생물학교실) ;
  • 김준만 (서울대학교 수의과대학 및 농생명공학 사업단 미생물학교실) ;
  • 노경민 (서울대학교 수의과대학 및 농생명공학 사업단 미생물학교실) ;
  • 허진 (서울대학교 수의과대학 및 농생명공학 사업단 미생물학교실) ;
  • 정우경 (서울대학교 수의과대학 및 농생명공학 사업단 미생물학교실) ;
  • 박건택 (서울대학교 수의과대학 및 농생명공학 사업단 미생물학교실) ;
  • 이종은 (RNL 생명과학) ;
  • 라정찬 (RNL 생명과학) ;
  • 박용호 (서울대학교 수의과대학 및 농생명공학 사업단 미생물학교실)
  • Kim, So-Hyun (Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
  • Kwon, Nam-Hoon (Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, J.Y. (Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
  • Lim, J.Y. (Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
  • Bae, W.K. (Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, J.M. (Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
  • Noh, K.M. (Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
  • Hur, J. (Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
  • Jung, W.K. (Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
  • Park, K.T. (Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
  • Lee, J.E. (RNL Life Science) ;
  • Ra, J.C. (RNL Life Science) ;
  • Park, Yong-Ho (Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University)
  • 발행 : 2002.06.01
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초록

항생제 및 항균 물질 등의 오, 남용으로 인하여 항생제 내성균들이 등장, 사회적으로 큰 문제가 되고 있으며, 최근에는 식물이나 probiotics 등과 같이 항생제를 대체할 수 있는 물질에 대한 연구가 활발히 진행되고 있다. 이에 본 연구에서는 국내에서 오래 전부터 민간 요법으로 사용되어 오던 백련초 (Opuntia ficus-indica var. saboten)를 이용하여 in vitro와 in vivo에서 Salmonella enteyica serova. Enteritidis (S. enteritidis), S. enterica serova. Typhimurium (S. Typhimurium) DT104 및 Escherichia coli O157:H7에 대한 항균능을 평가하였다 백련초의 수용액이 포함된 Moulter Hinton agar에 Salmonella spp. 및 S. coli O157:H7를 접종한 결과 이들 균에 대한 억제능(inhibitory activity)을 확인할 수 있었다. 백련초 제제를 경구투여한 군에서 분변으로 배출되는 S.typhimurium DT104의 수가 급격히 감소하였고, 낀 후에는 더 이상 분변으로 균이 배출되지 않았으며, intestinal IgA가 유의적으로 증가함이 확인되었다. 본 연구를 통하여 백련초가 in vitro에서 S. enteritis, S. Typhimurium DT104와 E. coli O157:H7대한 항균력을 나타낼 뿐만 아니라 마우스의 gastrointestinal tract에서 역시 Salmonella에 대한 항균력을 보임을 확인할 수 있었다. 본 연구를 통해 백련초 및 그 성분을 이용한 병원성 세균의 제거 및 면역 증진 효과 등과 같은 연구의 필요성이 확인되었고, 그 응용 가능성도 매우 높을 것으로 사료된다.

With the incidence of antibiotic resistant bacteria there is increasing interest in natural products such as herb extract and probiotics to control antibiotic resistant bacteria. This study was focused on the determination of antimicrobial activity of Opuntia ficus-indica var. saboten against Salmonella enetrica serovar Enteritidis (S. enterifidis), S. enterica serovar Typhimurium (S. Typhimurium) DT 104 and Escherichia coli 0157:H7. Though bactericidal effect of 0. ficus-indica var. saboten was not observed, it had significant inhibitory activity against Salmonella spp. and E. coli O157:H7 on the Moulter Hinton agar containing its solution dissolved in deionized water. To investigate the antimicrobial activity in vivo, mice were challenged with 5. Typhimurium DT104 (3.7$\times$108 cfu/mouse) after pre-feeding 0. ficus-indica var. saboten solution. The fecal shedding of S. Typhimurium DT104 was more dramatically decreased and not detectable in feces and intestines 3 days after challenge in mice fed with 0. ficus-indica var. saboten. Antibody responses of the intestinal IgA were also significantly increased in mice fed with 0. ficus-indica var. saboten. These findings suggest that Opuntia ficus-indica var. saboten decreased the shedding of S. Typhimurium DT104 in vitro and also in the gastrointestinal tract in mice. In addition, administration of the product might enhance the mucosal immune response against S. Typhimurium DT 104. In conclusion, Opuntia ficus-indica var. saboten might be useful to control antibiotic resistant bacteria in vivo and in vitro.

키워드

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