Animal cells and systems

  • 제6권2호
  • /
  • Pages.167-170
  • /
  • 2002
  • /
  • 1976-8354(pISSN)
  • /
  • 2151-2485(eISSN)
한국통합생물학회 (The Korean Society for Integrative Biology)
권호 표지

Identification and Phylogeny of the Human Endogenous Retrovirus HERV-W LTR Family in Cancer Cells

  • Yi, Joo-Mi (Division of Biological Sciences, College of Natural Sciences, Pusan University) ;
  • Kim, Hwan-Mook (Laboratory of Biopotency Evaluation, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Heui-Soo (Division of Biological Sciences, College of Natural Sciences, Pusan University)
  • 발행 : 2002.06.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The long terminal repeats (LTRs) of human endogenous retrovirus (HERV) have been found to be coexpressed with sequences of closely located genes. It has been suggested that the LTR elements have contributed to the structural change or genetic variation of human genome connected to various diseases and evolution. We examined the HERV-W LTR elements in various cancer cells (2F7, A43l , A549, HepG2, MIA-PaCa-2, PC-3, RT4, SiHa, U-937, and UO-31). Using genomic DNA from the cancer cells, we performed PCR amplification and identified twelve new HERV-W LTR elements. Those LTR elements showed a high degree of sequence similarity (88-99％) with HERV-W LTR (AF072500). A phylogenetic tree obtained by the neighbor-joining method revealed that HERV-W LTR elements could be mainly divided into two groups through evolutionary divergence. Three HERV-W LTR elements (RT4-2, A43l-1, and UO3l-2) belonged to Group 1, whereas nine LTR elements (2F7-2, A549-1, A549-3, HepG2-3, MP2-2, PC3-1, SiHa-8, SiHa-10, and U937-1) belonged to Group 11. Taken together, our new sequence data of the HERV-W LTR elements may contribute to an understanding of tissue-specific cancer by genomic instability of LTR integration.

키워드

참고문헌

  1. Akopov SB, Nikolaev LG, Khil PP, Lebedev YB, and Sverdlov ED (1998) Long terminal repeats of human endogenous retrovirus K family (HERV-K) specifically bind host cell nuclear proteins. FEBS Lett 421: 229-233 https://doi.org/10.1016/S0014-5793(97)01569-X
  2. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, and Lipman J (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25: 3389-3402 https://doi.org/10.1093/nar/25.17.3389
  3. Baust C, Seifarth W, Germaier H, Hehlmann R, and Leib-Mosch C (2000) HERV-K-T47D-related long terminal repeats mediate polyadenylation of cellular transcripts. Genomics 66: 98-103 https://doi.org/10.1006/geno.2000.6175
  4. Blond JL, Beseme F, Duret L, Bouton O, Bedin F, Perron H, Mandran B, and Mallet F (1999) Molecular characterization and placental expression of HERV-W, a new human endogenous retrovirus family. J Virol 73: 1175-1185
  5. Domansky AN, Kopantzev EP, Snezhkov EV, Lebedev YB, Leib-Mosch C, and Sverdlov ED (2000) Solitary HERV-K LTR possess bi-directional promoter activity and contain a negative regulatory element in the U5 region. FEBS Lett 472: 191-195 https://doi.org/10.1016/S0014-5793(00)01460-5
  6. Donner H, Tonjes RR, Bontrop RE, Kurth R, Usadel KH, and Badenhoop K (1999a) Intronic sequence motifs of HLA-DQB1 are shared between humans, apes and Old World monkeys, but a retroviral LTR element (DQLTR3) is human specific. Tissue Antogens 53: 551-558 https://doi.org/10.1034/j.1399-0039.1999.530605.x
  7. Donner H, Tonjes RR, Van der Auwera B, Siegmund T, Braun J, Weets I, Herwig J, Kurth R, Usadel KH, and Badenhoop K (1999b) The presence or absence of a retroviral long terminal repeat influences the genetic risk for type 1 diabetes conferred by human leukocyte antigen DQ haplotypes. J Clin Endocrinol Metab 84: 1404-1408 https://doi.org/10.1210/jc.84.4.1404
  8. Garson JA, Tuke PW, Giraud P, Paranhos-Baccaia G, and Perron P (1998) Detection of virione-associated MSRV-RNA in serum of patients with multiple sclerosis. Lancet 351: 33-33 https://doi.org/10.1016/S0140-6736(98)24001-3
  9. Kapitonov VV and Jurka J (1999) The long terminal repeat of an endogenous retrovirus induces alternative splicing and encodes an additional carboxy-terminal sequence in the human leptin receptor. J Mol Evol 48: 248-251 https://doi.org/10.1007/PL00013153
  10. Kim HS, Chen Y, and Lonai P (1998) Complex regulation of multiple cytohesin like genes in murine tissues and cells. FEBS Lett 433: 312-316 https://doi.org/10.1016/S0014-5793(98)00937-5
  11. Kim HS, Hirai H, and Takenaka O (1996) Molecular features of the TSPY gene of gibbons and Old World monkeys. Chrom Res 4: 500-506 https://doi.org/10.1007/BF02261777
  12. Kim HS and Lee YC (2001) Identification and phylogenetic analysis of long terminal repeat elements of the human endogenous retrovirus K family (HERV-K) from a human brain cDNA library. Korean J Biol Sci 5: 133-137
  13. King LM and Francomano CA (2001) Characterization of a human gene encoding nucleosomal binding protein NSBP1. Genomics 71: 163-173 https://doi.org/10.1006/geno.2000.6443
  14. Kjellman C, Sjogren H-O, Salford L, and Widegren B (1999) HERV-F (XA34) is a full-length human endogenous retrovirus expressed in placental and fetal tissues. Gene 239: 99-107 https://doi.org/10.1016/S0378-1119(99)00372-8
  15. Kumar S, Tamura K, and Nei N (1993) MEGA: Molecular Evolutionary Genetics Analysis, Version 1.01. The Pennsylvania State University, University Park
  16. La Mantia G, Maglione D, Pengue G, Di Cristofano A, Simeone A, Lanfrancone L, and Lania L (1991) Identification and characterization of a novel human endogenous retroviral sequences preferentially expressed in undifferential embryonal carcinoma cells. Nucleic Acids Res 19: 1513-1520 https://doi.org/10.1093/nar/19.7.1513
  17. Medstrand P, Landry JR, and Mager DL (2001) Long terminal repeats are used as alternative promoters for the endothelin B receptor and apolipoprotein C-I genes in humans. J Biochem 276: 1896-1903 https://doi.org/10.1074/jbc.M006557200
  18. O'Connell C, O'Brian S, Nash WG, and Cohen M (1984) ERV3, a full-length human endogenous provirus: chromosomal localization and evolutionary relationships. Virology 138: 225-235 https://doi.org/10.1016/0042-6822(84)90347-7
  19. Perron H, Garson JA, Bedin F, Beseme F, Paranhos-Baccala G, Komurian-Pradel F, Mallet F, Tuke PW, Voisset C, Blond JL, Lalande B, Seigneurin JM, Mandrand B, and the collaborative research group on MS (1997) Molecular identification of a novel retrovirus repeatedly isolated from patients with multiple sclerosis. Proc Natl Acad Sci USA 94: 7583-7588 https://doi.org/10.1073/pnas.94.14.7583
  20. Schon U, Seifarth W, Baust C, Hohenadl C, Erfle V, and Leib-Mosch C (2001) Cell type-specific expression and promoter activity of human endogenous retroviral long terminal repeats. Virology 279: 280-291 https://doi.org/10.1006/viro.2000.0712
  21. Sverdlov ED (1998) Perpetually mobile footprints of ancient infections in the human genome. FEBS Lett 428: 1-6 https://doi.org/10.1016/S0014-5793(98)00478-5
  22. Varmus HE (1982) Form and function of retroviral proviruses. Science 216: 812-820 https://doi.org/10.1126/science.6177038