Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Production and characterization of anti-thyrog1obulin monoclonal antibodies

Thyroglobulin에 대한 단일클론항체의 제작 및 특성

  • 남경수 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구센터) ;
  • 손윤희 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구센터) ;
  • 백태선 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구센터) ;
  • 김철호 (동국대학교 한의과대학 생화학교실) ;
  • 임종국 (동국대학교 한의과대학 경혈학교실) ;
  • 황철원 (한동대학교 환경미생물학교실)
  • Published : 2002.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

Twelve clones of monoclonal antibodies (mAbs) against thyroglobulin were produced and characterized. Among them, three mAbs (TN-1, TN-2 and TN-3) showed high binding affinity to thyroglobulin. from ELISA inhibition assay, TN-2 showed considerable reactivity with soluble thyroglobulin. TN-2 also reacted with phosphatidylserine which has a negative charge in aqueous condition. These results suggest that TN-2 has characteristics of autoantibody concerned with thyroiditis.

Thyroglobulin과 반응하는 monoclonal antibody를 3클론(TN-1, TN-2 및 TN-3)을 확립하였다. 이들 중 1클론 (TN-2)은 thyroglobulin의 3차원적인 구조를 인식하면서 음전하를 띠는 자가항원의 일종인 phosphatidylserine과도 교차반응성을 나타내었다. 따라서 TN-2를 이용한 자가면역성 갑상선염의 진단 및 치료에의 적용효과가 기대된다.

Keywords

References

  1. Ann.Rev.Med. v.39 Antiphospholipid antibodies-autoantibodies with a difference Harris,E.N.;R.A.Ashersom;G.R.V.Hughes https://doi.org/10.1146/annurev.me.39.020188.001401
  2. THROMBOSIS RESEARCH v.61 Effective induction of anti phospholipid and anticoagulant in normal mouse Igarashi,K.;M.Umeda;S.Tokida;K.S.Nam;K.Inoue https://doi.org/10.1016/0049-3848(91)90240-W
  3. Endocr.J. v.46 A case of Hashimoto's thyroiditis with markedly elevated serum throglobulin of anti-thyrogloblin antibody by highly sensitive assay Moriyama,K.;T.Akamizu;M.Umemoto;M.Miura;M.Saijo;K.Taniguchi;K.Natao https://doi.org/10.1507/endocrj.46.687
  4. Biochim.Biophys.Acta v.1046 Production and characterization of monoclonal antibodies that specifically bind to phosphatidyp choline Nam,K.S.;K.Igarashi;M.Umeda;K.Inoue https://doi.org/10.1016/0005-2760(90)90098-I
  5. Pharm.Bull v.16 Production and characterization of monoclonal antibodies that simultaneusly recognizes methanphetamin and its major metabolite Biol Nam,K.S.;J.W.Kim;M.J.Choi;M.Y.Choi;I.S.Choe;T.W.Chung https://doi.org/10.1248/bpb.16.490
  6. Korean J. Immunol v.14 Production mechanism of anti-phospholipid antibody and analysis of its recognition profile Nam,K.S.
  7. Eur J. Immunol v.14 Monoclonal anti-cardiolipin antibodies bind ro DNA Rauch,J.;H.Tannenbaum;B.D.Stollar;R.S.Schwartz https://doi.org/10.1002/eji.1830140609
  8. Proc.Natl.Acad,Sci.USA v.84 Structure and function of anti-DNA autoantibodies derived from a single autoimmune mouse Shlomchik,M.J.;A.H.Aucoin;D.S.Pisetesky;M.G.Weigert https://doi.org/10.1073/pnas.84.24.9150
  9. Mol.Immunol. v.26 Cross-reactions of nucleic acids with monoclonaln antobodies to phosphatidylinositol phosphate and cholesterol Stollar,B.D.;T.Mcinerney;T.Gavron;N.M.Wassef;G.M.Swartz;C.R.Alving https://doi.org/10.1016/0161-5890(89)90022-9
  10. J. Immunol v.143 Effective production of monoclonal antibodies against phosphatidylserine :stereo-specific recognition of phosphatidylserine by monoclonal antibody Umeda,M.;K.Igarahsi;K.S.Nam;K.Inoue