생명과학회지 (Journal of Life Science)

  • 제12권4호
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  • Pages.460-463
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  • 2002
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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Thyroglobulin에 대한 단일클론항체의 제작 및 특성

Production and characterization of anti-thyrog1obulin monoclonal antibodies

  • 남경수 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구센터) ;
  • 손윤희 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구센터) ;
  • 백태선 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구센터) ;
  • 김철호 (동국대학교 한의과대학 생화학교실) ;
  • 임종국 (동국대학교 한의과대학 경혈학교실) ;
  • 황철원 (한동대학교 환경미생물학교실)
  • 발행 : 2002.08.01
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초록

Thyroglobulin과 반응하는 monoclonal antibody를 3클론(TN-1, TN-2 및 TN-3)을 확립하였다. 이들 중 1클론 (TN-2)은 thyroglobulin의 3차원적인 구조를 인식하면서 음전하를 띠는 자가항원의 일종인 phosphatidylserine과도 교차반응성을 나타내었다. 따라서 TN-2를 이용한 자가면역성 갑상선염의 진단 및 치료에의 적용효과가 기대된다.

Twelve clones of monoclonal antibodies (mAbs) against thyroglobulin were produced and characterized. Among them, three mAbs (TN-1, TN-2 and TN-3) showed high binding affinity to thyroglobulin. from ELISA inhibition assay, TN-2 showed considerable reactivity with soluble thyroglobulin. TN-2 also reacted with phosphatidylserine which has a negative charge in aqueous condition. These results suggest that TN-2 has characteristics of autoantibody concerned with thyroiditis.

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참고문헌

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