생명과학회지 (Journal of Life Science)

  • 제12권4호
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  • Pages.427-432
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  • 2002
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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한국산 잇바디돌김 (Porphyra dentata)의 핵 18S rDNA 염기선열 분석

Sequence Analysis of Nuclear 18s rDNA from Porphyra dentata (Rhodophyta) in Korea

  • 발행 : 2002.08.01
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초록

잇바디돌김(Porptyra dentata)을 대상으로 핵의 18S ribo-somal RNA를 지령하는 유전자 즉 18S rDNA 유전자를 증폭하고, 염기서열분석을 수행하였다. 전체 18S rDNA의 exon 영역 크기는 1822 bp, intron 영역의 크기는 512 bp였다. 이들 exon과 intron 영역의 G+C함량은 각각 49%와 55%씩 나타내었다. 일본산 잇바디돌김(CenBank accession number: AB013183)의 exon 영역과의 비교에서 상동성이 97.1%에 도달하였다. 568번과 569번 염기사이의 upstream에 위치하는 intron 영 역에서는 AB013183과 52.1%의 상동성을 보였다.

Nuclear 18S ribosomal RNA gene (18S rDNA or SSU rDNA) from the Porphyra dentata tissue was amplified and sequenced. Complete 18S rDNA has an 1822 bp exon and a 512 bp intron. The G+C contents of exon and intron were 49% and 55%, respectively. The exon sequence showed 97.1% homology to the GenBank accession number AB013183 of the Japanese P. dentata. The intron region that is inserted in upstream between 568 and 569 showed 52.1% homology to the AB013183.

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피인용 문헌

  1. Composition of Amino Acid and Photosynthesis Pigment on Growing Season of Pyropia dentata (Rhodophyta) in Haenam, Korea vol.30, pp.4, 2018, https://doi.org/10.13000/JFMSE.2018.08.30.4.1248