Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Sequence Analysis of Nuclear 18s rDNA from Porphyra dentata (Rhodophyta) in Korea

한국산 잇바디돌김 (Porphyra dentata)의 핵 18S rDNA 염기선열 분석

  • Published : 2002.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

Nuclear 18S ribosomal RNA gene (18S rDNA or SSU rDNA) from the Porphyra dentata tissue was amplified and sequenced. Complete 18S rDNA has an 1822 bp exon and a 512 bp intron. The G+C contents of exon and intron were 49% and 55%, respectively. The exon sequence showed 97.1% homology to the GenBank accession number AB013183 of the Japanese P. dentata. The intron region that is inserted in upstream between 568 and 569 showed 52.1% homology to the AB013183.

잇바디돌김(Porptyra dentata)을 대상으로 핵의 18S ribo-somal RNA를 지령하는 유전자 즉 18S rDNA 유전자를 증폭하고, 염기서열분석을 수행하였다. 전체 18S rDNA의 exon 영역 크기는 1822 bp, intron 영역의 크기는 512 bp였다. 이들 exon과 intron 영역의 G+C함량은 각각 49%와 55%씩 나타내었다. 일본산 잇바디돌김(CenBank accession number: AB013183)의 exon 영역과의 비교에서 상동성이 97.1%에 도달하였다. 568번과 569번 염기사이의 upstream에 위치하는 intron 영 역에서는 AB013183과 52.1%의 상동성을 보였다.

Keywords

References

  1. J.Mol.Biol v.215 Basic local alignement search tool. Altschul,S.F.;W.Gish;W.Miller; E.W.Mayers;D.J.Limpman https://doi.org/10.1016/S0022-2836(05)80360-2
  2. Nucl. Acids. Res. Inc v.25 Gapped BLAST and PSI-BLAST:a new generation of protein database search programs Altschul,S.F.;T.L.Madden;A.A.Schaffer;J.Zhang;W.Miller;D.J.Limpman https://doi.org/10.1093/nar/25.17.3389
  3. Introduction or the Algae Bold,H.C.;M.J.Wynne
  4. J.Appl.Phycol. v.7 DNA extraction conditions from Porphyra perforata using LiCi Hong,Y.K.;S.D.Kim;M.Polne-Fuller;A.Gibor https://doi.org/10.1007/BF00693055
  5. Algae v.16 Taxonomy of the genus Porphyra(Bangiales, Rhodophyta) from Korea Hwang,M.S.;I.K.Lee
  6. Trends Biochem Sci v.23 Multiple sequence alignment with Clustal X. Jaennmougin,F.;J.D. Thompson;M.Gouy;D.G.Higgins;T.J.Gibson https://doi.org/10.1016/S0968-0004(98)01285-7
  7. Korean Fisheries Yearbook Korea Fisheries Association
  8. J.Appl.Phycol. v.11 Discrimination of Porphyra species based on small subunit ribosomal RNA gene sequence Kunimoto,M.;H.Kito,Y.Yamamoto;D.P.Cheney;Y.Kaminishi;Y.Mizukami https://doi.org/10.1023/A:1008019409008
  9. EMBNEW News v.4 GeneDoc:Analysis and visualization of genetic varivation Nicholats,K.B.;H.B.Nicholas Jr;D.W.Deerfield
  10. J.Mar.Biotechnol v.6 RAPD identification of genetic variation in the seaweed Hizikia fusiformis Park,J.W.;Y.C.Cho;B.H.Nam;H.J.Jin;C.H.Sohn;Y.K.Hong
  11. Molecular cloning:A laboratory manual(2nd eds.) Sambrook,J.;E.F.Fritsch;T,Maniatis
  12. J.Phycol. v.29 Molecular analysis reveals crytic diversity in Prophyra(Rhodophyta) Stiler,J.W.;J.R.Waa;amd https://doi.org/10.1111/j.1529-8817.1993.tb00152.x
  13. PCR protocols: A guids to methods and applications Amplification and direct seqeuncing of fungal ribosomal RNA genes for phylogenetics White,T.J.;T.Bruns;S.Lee;J.Taylor;Innis,M.;J.Gelfand(eds.);J.Sninsky(eds.);T.White(eds.)

Cited by

  1. Composition of Amino Acid and Photosynthesis Pigment on Growing Season of Pyropia dentata (Rhodophyta) in Haenam, Korea vol.30, pp.4, 2018, https://doi.org/10.13000/JFMSE.2018.08.30.4.1248