생명과학회지 (Journal of Life Science)

  • 제12권4호
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  • Pages.399-406
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  • 2002
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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Diepoxybutane에 의해 유도된 애기장대 엽록체 돌연변이체의 분석

Analysis of Chloroplast Mutants of Arabidopsis Induced by Diepoxybutane

  • 윤용휘 (경북대학교 농과대학 농학과) ;
  • 이정훈 (경북대학교 농과대학 농학과) ;
  • 박해진 (경북대학교 농과대학 농학과) ;
  • 강용원 (경북대학교 농과대학 농학과) ;
  • 이경민 (경북대학교 농과대학 농학과) ;
  • 신동현 (경북대학교 농과대학 농학과) ;
  • 이인중 (경북대학교 농과대학 농학과) ;
  • 김학윤 (계명대학교 환경학부) ;
  • 김달웅 (경북대학교 농과대학 농학과)
  • 발행 : 2002.08.01
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초록

본 연구에서는 DEB를 애기장대 종자에 처리하여 엽록체 발달에 관련된 돌연변이체들을 분리하였다. 이들은 각각 그 특징에 따라 iml, gev, yev로 명명하였으며 iml은 잎에 얼룩무늬를 나타내는 돌연변이체이며, gev는 엽맥에만 녹색을 나타내고 이외의 잎에서는 연한녹색을 나타내며, yev는 엽맥은 연한녹색을 나타내고 엽맥 이외의 잎에서는 녹색을 나타내었다. 주사 전자현미경을 이용하여 엽록체 상세구조를 관찰하여 본 결과 야생형 엽록체의 그라나 티라코이드의 모양은 규칙적인 배열하는 그라나가 쌓여 있었으나, iml, gev, yev 돌연변이체 에서는 그라나 티라코이드의 모양이 불규칙하며 그라나의 수도 불규칙하며 스트로마 티라코이드의 연결도 야생형과 상이함을 보였다. 그리고 이들을 유전분석한 결과 야생종과 돌연변이체의 F$_2$에서 3:1의 분리비를 나타내어, 이들 돌연변이들은 핵내에 존재하는 유전자에서 돌연변이가 일어났음을 알 수 있었고, 단일열성으로 유전됨이 밝혀졌다.

The Arabidopsis mutants involved in chloroplast development were induced by seed treatment of diepoxybutane which was rarely known mutagenic compound in plant mutagenesis. Three kinds of mutants designated as iml, gev, and yev were represented by the characteristics of variegated leaves, green vein with yellow leaves, and yellow green vein with green leaves respectively. We investigated the ultrastructure of chloroplast in mutated regions using transmission electron microscopy. The ultrastructure of chroloplast in wildtype showed regularly stacked grana thylakoid and stroma thylakoid while iml, gev and yev mutants displayed different shapes of grana stacking and stroma stacking of chloroplasts. Genetic analysis of three chloroplast mutants exhibit that divergent traits were ruled by a single recessive nuclear gene.

키워드

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