생명과학회지 (Journal of Life Science)

  • 제12권4호
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  • Pages.392-398
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  • 2002
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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Lignocellulose의 분해 및 이용을 위한 Lignin 분해 세균의 분리

Isolation of a Lignolytic Bacterium for Degradation and Utilization of Lignocellulose

  • 발행 : 2002.08.01
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초록

Lignocellulose 분해 이용을 위해 토양 등에서 lignin분해 능력이 우수한 세균을 분리 및 동정함으로서 방향족 화합물의 이용을 위한 생물전환의 기초를 확보하고자하였다. 토양 등의 시료로부터 38주의 리그닌 분해 균주를 분리하였다. 분리된 LG2를 공시 균주로 선정하여 형태학적, 배양학적 및 생리학적 특성을 조사한 결과 Pseudomonas sp. LG2로 명명하였다. 이 균주는 리그닌을 분해 할 수 있었으며, 리그닌 함유 배지에 배양하여 배양 분해 산물을 HPLC로 분석한 결과 다수의 방향족 화합물을 생산하였다 Poylacrylamide gel활성 분석에 의해서 3종류로 구성된 peroxidase가 조사되었다.

38 strains were isolated in order to utilize lignin degrading ability from soil and compost. A organism having high lignin degrading ability of the isolated strains determined morphologcal and biochemical characteristics. Enrichment technique yielded a lignin degrading bacterium characterized as Pseudomonas sp. LC-2. This strain was able to degrade lignin which are the true representatives of native lignin and transform lignin to a lot of aromatic compounds as HPLC analysis of culture. By polyacrylamide gel analysis, it was determined that peroxidase consisted of three enzymes, with only one, the lignin peroxidase having high activity.

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참고문헌

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피인용 문헌

  1. Partial Characterization and Induction of Ferulic Acid Esterase and Xylanase from Pseudomonas sp. LG2 vol.17, pp.4, 2007, https://doi.org/10.5352/JLS.2007.17.4.568
  2. Phylogenetic characteristics of bacterial populations and isolation of aromatic compounds utilizing bacteria from humus layer of oak forest vol.52, pp.2, 2016, https://doi.org/10.7845/kjm.2016.6028