Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Enhanced Biodegradation of 2,4,6-Trinitrotoluene (TNT) with Various Supplemental Energy Sources

  • Park, Chulhwan (Advanced Energy & Enviornment Research Team, Korea Institute of Industrial Technology (KITECH), Department of Chemical and Biological Engineering, Korea University) ;
  • Kim, Tak-Hyun (Advanced Energy & Enviornment Research Team, Korea Institute of Industrial Technology (KITECH)) ;
  • Kim, Sangyong (Advanced Energy & Enviornment Research Team, Korea Institute of Industrial Technology (KITECH)) ;
  • Kim, Seung-Wook (Department of Chemical and Biological Engineering, Korea University) ;
  • Lee, Jinwon (Department of Chemical Engineering, Kwangwoon University)
  • Published : 2002.08.01
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Abstract

The biodegradation of 2,4,6-trinitrotoluene (TNT) was performed on a laboratory scale using P. putida originally isolated from explosive-contaminated soil. One hundred mg/1 of TNT was completely degraded within 20 h under optimum conditions. Various supplemental energy sources (carbon sources, nitrogen sources, and surfactant) were tested, with the main objective of identifying an inexpensive source and enhancing the degradation rate for large-scale biodegradation. Based on the degradation rate, molasses was selected as a possible supplemental carbon source, along with NH$_4$Cl and Tween 80 as a nitrogen source and surfactant, respectively. The degradation rate increased about 3.3 fo1d when supplemental energy sources were added and the degradation rate constant increased from 0.068 h$\^$-1/ to 0.224 h$\^$-1/. These results appear to be promising in application of the process to TNT-contaminated soil applications.

Keywords

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