Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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cDNA Microarray Analysis of Transcriptional Response to Hyperin in Human Gastric Cancer Cells

  • Jeoung, Dooil (Cancer Genomics Unit, In2gen Company) ;
  • Kim, Jae-Hwan (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University) ;
  • Lee, Youn-Hyung (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University) ;
  • Myungin Baek (Cancer Genomics Unit, In2gen Company) ;
  • Lee, Seongeun (Cancer Genomics Unit, In2gen Company) ;
  • Baek, Nam-In (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University Company) ;
  • Kim, Hae-Yeong (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University)
  • Published : 2002.08.01
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Abstract

Antioxidants either scavenge superoxide and free radicals or stimulate the detoxification mechanisms within cells, resulting in increased detoxification of free radicals formation. Hyperin, isolated from the stem of Uncaria rhynchophylla, prevented oxygen radical formation and inhibited lipid oxidation. The effective concentrations were 31.3 $\mu$M for a radical scavenging assay and 2.2 $\mu$M for a microsome assay. cDNA microarray analysis to determine which genes were modulated by hyperin found that 50 genes were upregulated and 37 genes were downregulated in SNU-668 human gastric cancer cells. Among these genes, thirteen genes that were significantly affected by hyperin were verified by RT-PCR for their effect of genetic reprogramming.

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