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유통 생식제품의 미생물 분포 및 감마선 조사를 이용한 위생화

Distribution of Microflora in Powdered Raw Grains and Vegetables and Improvement of Hygienic Quality by Gamma Irradiation

  • 김동호 (한국원자력연구소 방사선식품·생명공학연구팀) ;
  • 송현파 (한국원자력연구소 방사선식품·생명공학연구팀) ;
  • 육홍선 (충남대학교 식품영양학과) ;
  • 정영진 (충남대학교 식품영양학과) ;
  • 김영지 (영남이공대학 식품영양과) ;
  • 변명우 (한국원자력연구소 방사선식품·생명공학연구팀)
  • Kim, Dong-Ho (Team for Radiation Food Science and Biotechnology, Korea Atomic Energy Research Institute) ;
  • Song, Hyun-Pa (Team for Radiation Food Science and Biotechnology, Korea Atomic Energy Research Institute) ;
  • Yook, Hong-Sun (Dept. of Food Nutrition, Chungnam National University) ;
  • Chung, Young-Jin (Dept. of Food Nutrition, Chungnam National University) ;
  • Kim, Yeung-Ji (Dept. of Food Science and Nutrition, Yeungnam College of Science and Technology) ;
  • Byun, Myung-Woo (Team for Radiation Food Science and Biotechnology, Korea Atomic Energy Research Institute)
  • 발행 : 2002.08.01

초록

유통 생씩 5종의 수분 함량 4.22~7.18%의 범위였으며 수분활성은 0.15~0.26의 분포를 보여 미생물에 의한 변패보다는 낮은 수분활성에서도 생존해 있는 병원성 미생물의 제거가 생식의 중요한 품질관리 요소일 것으로 판단된다. 생식의 미생물분포는 Bacillus group $10^4$~$10^{7}$ cfu/g, 사상균류 $10^2$~$10^3$cfu/g, coliform group $10^1$~ $10^4$cfu/g, SS agar plate 분리 enteric bacteria group $10^1$~$10^3$cfu/g의 수준으로 특히 병원성 미생물의 오염가능성이 컸다. 감마선 조사 결과 coliform group, SS agar plate 분리 enteric bacteria group, 그리고 사상균류는 3 kGy의 조사선량에서 완전살균 수준으로 제거되었다. 생식 분포 미생물의 D값은 coliform group은 0.68~0.80 kGy, SS agar plate 분리 enteric bacteria group은 0.59~0.74 kGy, Bacillus group은 1.84~2.18 kGy, 사상균류는 0.36~0.57 kGy의 범위를 나타내었다. 생식제품의 위생화를 목적으로 할 경우 감마선조사 선량은 생식제품에 분포하는 coliform group과 SS agar plate 분리 enteric bacteria group 미생물의 사멸기준인 3~5 kGy의 수준으로 설정하는 것이 바람직할 것으로 사료된다.

Improvement of hygienic quality of powdered raw grains and vegetables by gamma irradiation was investigated. Five products of powdered raw grains and vegetables were collected in a local market and analysed. The total viable cell counts of Bacillus were 10$^4$~10$^{7}$ cfu/g, filamentous fungi l0$^2$~10$^3$cfu/g, coliform 10$^1$~10$^4$cfu/g, enteric bacteria on SS agar plate 10$^1$~10$^3$cfu/g. Coliform, enteric bacteria on SS agar plate and filamentous fungi were eliminated by 3 kGy of gamma irradiation. The D values of coliform, enteric bacteria on SS agar plate, Bacillus, and filamentous fungi were 0.68~0.80 kGy, 0.59~0.74 kGy, 1.84~2.18 kGy and 0.36~0.57 kGy, respectively. It was considered that optimal irradiation dose for radappertization was about 25 kGy, while 3~5 kGy of gamma irradiation was effective for radicidation.

키워드

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