한국양식학회지 (Journal of Aquaculture)

한국양식학회 (The Korean Society of Fisheries and Aquatic Science)
권호 표지

치어기 조피볼락에 있어서 사료내 합성아미노산 첨가 효과

Effects of Dietary Synthetic Amino Acid Supplementation in Korean Rockfish Fry Sebastes schlegeli

  • 김강웅 (부경대학교 양식학과, 사료영양연구소) ;
  • 박건준 (부경대학교 양식학과, 사료영양연구소) ;
  • 옥임호 (부경대학교 양식학과, 사료영양연구소) ;
  • 배승철 (부경대학교 양식학과, 사료영양연구소) ;
  • 최영준 (경상대학교 해양생물이용학부) ;
  • 신인수 (미국대두협회)
  • 발행 : 2002.08.01
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초록

본 실험은 섭취촉진물질로 알려져 있는 사료내 13종의 아미노산들이 치어기 조피볼락에 있어 성장 및 체조성에 어떠한 영향을 미치는지 알아보고자 실시하였다 실험어는 평균무게 0.64 g인 조피볼락 치어를 사용하였으며, 기초실험 사료내에 유인효과가 있을 것으로 여겨지는 아미노산 13가지(Arg, Ala, Na-Glu, Gly, His, Ile, Lys, Met, Phe, Pro, Thr, Trp 및 Val)를 사료내 각각 0.3% 씩 첨가하여 총 13 실험구를 2반복으로 나누어 6주간 사육하였다. 6주간의 사육실험 결과, 성장률(WG)과 일간 성장률(SGR)에서는 Pro, Thr, Met 및 Gly 실험구가 다른 실험구에 비해 유의적으로 높았으나(P<0.05), 이들 실험구간에는 유의적인 차이가 없었다(P>0.05). 그리고, 사료전환효율(FE)에서는 Pro 실험구가 Met과 Gly 실험구보다 유의적으로 높았으며(P<0.05), Pro 실험구와 Thr 실험구 사이에는 유의적인 차이가 없었다(P>0.05). 그리고 Thr, Met 및 Gly 실험구간에도 유의적인 차이가 없었다(P>0.05). 단백질전화효율에 있어서 Pro 실험구가 모든 실험구에 있어서 유의적으로 가장 높았으며(P<0.05), Thr 실험구와 비교하여 유의적으로 차이가 나지 않았다(P>0.05). 그리고, 비만도(condition factor)는 Thr 실험구가 유의적으로 가장 높았으며(P<0.05), Val 실험구는 가장 낮게 나타났다(P>0.05). 간중량지수(HSI)는 모든 실험구간에서 유의적인 차이가 나지 않았다(P>0.05). 전어체의 단백질, 수분 및 회분에 있어서는 모든 실험구에 있어서 유의적인 차이가 나지 않았다(P>0.05). 지질함량은 Met, Pro 및 Thr 실험구가 Glu, Gly, Lys, Trp 및 Val 실험구보다 유의적으로 낮았다(P>0.05). 상기 결과를 토대로, 조피볼락 사료용 사료섭취촉진물질로서 아미노산을 검색한 결과 사료내 0.3% Pro, Thr, Met 및 Gly을 첨가했을 때 사료섭취촉진 효과가 나타났으며, 이것들 중 Gly을 제외하고는 전어체의 지질 함량을 감소시키는 결과를 나타내었다.

Thirteen groups, each consisting of 25 juveniles (0.64 g) of the Korean rockfish, Sebastes schlegeli were reared in aquaria after period of one week conditioning. Each group was fed with one of the 13 diets at the rate of 7~10% body weight (on a dry-matter basis) 2 times a day for 6 weeks. Each diet was supplemented with one of the following amino acids at 3g/kg diet: Alanine (Ala), Arginine (Arg), Glycine(Gly), Glutamate (Glu), Histidine (His), Isoleusine(Ile), Lysine (Lys), Methionine (Met), Phenylalanine (Phe), Proline (Pro), Threonine (Thr), Tryptophan (Trp) or Valine (Val). Groups fed with Pro, Thr, Met or Gly-supplemented diet showed significantly (p<0.05) higher weight gain and faster specific growth rate than the groups fed on other diets, while those fed Pro, Thr, Met or Gly were not significantly different each other(P>0.05). Feed efficiency (FE) of fish fed Pro was significantly higher than those fed the other diets except that fed Thr (P<0.05). However, there was no significant difference between FE of fish fed Pro and Thr, and among FE of fish fed Thr, Met and Gly (P>0.05). The requirement of rockfish is higher for Met and Thr. Extra Pro and/or Gly may also stimulate biosynthesis of the nucleic acids (IMP, GMP) which are known as the feed stimulant in fish.

키워드

참고문헌

  1. J.Fish Biol. v.12 Studies on the chemical nature of feeding stimulants for rainbow trout Adron J.W.;A.M.Mackie https://doi.org/10.1111/j.1095-8649.1978.tb04175.x
  2. Bull.Jpn.Soc.Sci.Fish v.51 Threonine,histidine and lysine requirements of chum salmon fry Akiyama T.;S.Arai;T.Murai;T.Nose https://doi.org/10.2331/suisan.51.635
  3. Association of Official Analytical Chemists Official methods of analysis(16th ed) AOAC
  4. Journal of World Aquaculture Society v.26 no.3 Dietary threonine requirement of juvenile red drum(Sciaenops ocellaus) Boren R.S.;D.M.Gatlin III https://doi.org/10.1111/j.1749-7345.1995.tb00256.x
  5. Orthopristis chrysopterus:Characterization and identification of stimulatory substance in a shrimp extract v.55A Chemoreception and feeding behavior in the pigfish Carr W.E.S.
  6. Chemoreception in Fishes Chemical stimulation of feeding behaviour Carr W.E.S.;T.J.Hara(ed.)
  7. J.Nutr. v.76 Nutrition of salmonid fishes.10.Quantitative threonine requirements of chinook salmon at two water temperatures Deolng D.C.;J.E.Halver;E.T.Mertz
  8. Morone saxatilis.Aquaculture v.185 Identification of feeding stimulants for striped bass Elias P.;H.S.Joesph
  9. Perinereis brevicirrus.Bull.of Jap.Soc.Sci.Fish v.47 Indentification of feeding stimulants for red sea bream in the extract of marine worm Fuke S.;S.Konosu;K.Ina https://doi.org/10.2331/suisan.47.1631
  10. Seriola quinqueradiata Nippon Suisan Gakkaishi v.55 no.5 The feeding stimulatory effects of squid muscle extracts on the young yellowtail Fukuda K.;J.Kohbara;C.Zeng;I.Hidaka https://doi.org/10.2331/suisan.55.791
  11. Prog.Fish-Cult. v.39 Effects of dietary carbohydrate-to-lipid ratios on growth and body composition of fingerling channel catfish Garling D.L.Jr.;R.P.Wilson https://doi.org/10.1577/1548-8659(1977)39[43:EODCRO]2.0.CO;2
  12. Methionine and cystine requriements of chinook salmon.Fed.Proc. Halver J.E.;D.C.DeLong;E.T.Mertz
  13. Bull.Japan.Soc.Sci.Fish v.51 no.3 Feeding attraction activities of amino acids and lipids for juvenile yellowtail Harada K. https://doi.org/10.2331/suisan.51.453
  14. Bull.Japan.Soc.Sci.Fish v.52 no.11 Feeding attraction activities of mucleic acid-related compounds for abalone,oriental weather fish and yellowtail Harada K. https://doi.org/10.2331/suisan.52.1961
  15. Bull.Japan.Soc.Sci.Fish v.50 no.4 Feeding attractants in chemical constituents of lake prawn for oriental weather fish Harada K.;I.Ikeda https://doi.org/10.2331/suisan.50.617
  16. Bull.Japan.Soc.Sci.Fish v.51 no.12 Feeding attraction activities of proteins,amino acids,lipid and nitrogenous bases for abalone Harada K.;Y.Akishima https://doi.org/10.2331/suisan.51.2051
  17. Nippon Suisan Gakkaishi v.58 no.8 Effect of attract and repellent mixtures on behavior of the oriental weather fish Misgurnus anguillicandatus Harada K. https://doi.org/10.2331/suisan.58.1427
  18. Nippon Suisan Gakkaishi v.54 no.2 Identification of feeding stimulant in the krill extract for jack mackerel Ikeda I.;H.Hosokawa;S.Shimeno;M.Takeda https://doi.org/10.2331/suisan.54.235
  19. Seriola quinqueradiata.Aquaculture v.181 no.1-2 Identification of feeding stimulants from a jack mackerel muscle extract for young yellowtail Iwao H.;K.Jun;A.Toshiyoshi;M.Tatsuo;M.Toshiaki;S.Shigeki;K.Isao
  20. J.of Ichthyology v.36 Taste sensitivity of common carp (Cyprinus carpio) to free amino acids and classical taste substances Kasumyan A.O.;A.M.K.Morsi
  21. Aquaculture v.104 Dietary lysine requirement of juvenile hybrid striped bass(Morone chrysops x M.saxatilis) Keembiyehetty C.N.;D.M.Gatlin III
  22. Sebastes schlegeli.J.of Aquaculture v.10 no.2 Fish meal analog as a dietary protein source in Korean rockfish Kim K.W.;S.C.Bai
  23. Seriola qunigueradiata.Nippon Suisan Gakkaishi v.55 no.8 The feeding stimulatory effects of jack mackerel muscle extracts on the young yellowtail Kohbara J.;K.Fukuda;I.Hidaka https://doi.org/10.2331/suisan.55.1343
  24. Symposium on Brackish water Aquaculture v.52 Preliminary study of the protein requriements of the gilthead bream(Chrysophrys aurata) Luquet P.;J.J.Sabaut
  25. Watanabe and lipid levels on performance and carcass composition of fingerling carp.Bull.Jap.Soc.Sci.Fish v.54 Murai T.;T.Akiyama;T.Watanabe;T.Nose
  26. Aquaculture v.150 no.1-2 Quantitative methionine requirement of yellowtail(Seriola quinqueradiata) Ruchimat T.;T.Masumoto;H.Hosokawa;S.Shimeno https://doi.org/10.1016/S0044-8486(96)01465-2
  27. J.of Fish Biology v.53 no.6 Behavioural responses of glass eels Anguilla anguilla to nonprotein amino acids Sola C.;P.Tongiorgi
  28. Institut national de la recherche agronomique L-ascorbyl-2-phosphate-mg as vitamin C source for the Japanese flounder(Paralichthys olivaceus) Teshima S.I.;A.Kanazawa;S.Koshio;S.Itoh