한국축산식품학회지 (Food Science of Animal Resources)

  • 제22권2호
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  • Pages.164-171
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  • 2002
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  • 2636-0772(pISSN)
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  • 2636-0780(eISSN)
한국축산식품학회 (Korean Society for Food Science of Animal Resources)
권호 표지

복합 박테리오신의 항균활성 및 축산식품 저장성 증진 효과

Antimicrobial Effects of a Bacteriocin Mixture from Lactic Acid Bacteria against Foodborne Pathogens

  • 한경식 (고려대학교 식품과학부) ;
  • 오세종 ((주)한국야쿠르트 중앙연구소) ;
  • 문용일 (우석대학교 생명자원학부)
  • 발행 : 2002.06.01
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초록

박테리오신을 생산하는 9종의 유산균 배양액으로부터 ammonium sulfate를 첨가하여 제조된 각각의 조박테리오신을 혼합하여 복합박테리오신용액을 제조하였다. 복합박테리오신의 항균 활성은 단일 박테리오신보다 우수하였으며 항균 범위 또한 넓어짐을 알 수 있었고 단일 박테리오신에 대하여 저항성을 나타내는 Listeria monocytogenes의 생육을 저해하는 것으로 나타났다. 또한, 복합박테리오신은 pH와 열에강한 안정성을 보여 식품 가공 중에도 이용이 가능한 것으로 나타났다. 복합박테리오신을 프랑크소세지, 모짜렐라 치즈 및 돈육등심근에 첨가한 후 저장기간별 일반세균수의 변화를 조사한 결과, 대조구에 비해 유의적인 감소현상을 나타내었고 저장 28일 경과 후에는 모든 식품에서 1/10 또는 1/100 정도로 식품내 총세균이 억제되었다. 또한, 저장기간 중 VBN의 함량을 조사한 결과 돈육등심근과 모짜렐라 치즈의 경우 14일 이후부터 박테리오신 처리구가 대조구에 비해 유의적으로 낮은 수치를 보여주었다.

The purpose of this study was to evaluate inhibitory activity of a bacteriocin mixture from lactic acid bacteria(LAB) against foodborne pathogens. Each bacteriocin solutions were prepared by growing nine strains of bacteriocin producers in MRS broth for 18~24 h followed by centrifugation(8000$\times$g, 20 min, 4$^{\circ}C$). Bacteriocins were purified from ammonium sulfate precipitation and were resuspended in 50 mM phosphate buffer(pH 7.0). Nine bacteriocins were mixed together and then allowed to freeze at -2$0^{\circ}C$. The mixture of nine bacteriocins showed enhanced inhibitory activity compared to each of bacteriocins and inhibited the Gram negative pathogens including Escherichia coli 0157:H7, Klebsiella pneumoniae, Pseudomonas chlororaphis and Shigella sonnei. The mixture of bacteriocin solutions was significantly lower than controls when a freeze-dried bacteriocin mixture was added to frank sausage, Mozzarella cheese and pork loin. With addition of bacteriocin mixture, total mesophilic bacteria in pork loin were constant over storage period, whereas total mesophilic bacteria in Mozzarella cheese and frank sausang slightly increased. Total viable cells of control group increased during storage without bacteriocin treatment. Volatile base nitrogen content of pork loin during storage also increased significantly without bacteriocin treatment. The bacteriocin mixture was capable of inhibiting pathogenic and spoilage microorganisms and extending the shelf-life of cheese and meat products during storage.

키워드

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