Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Rapid Separation of Cellular Cyclosophoraoses Produced by Rhizobium Species

  • Seo, Dong-Hyuk (Department of Microbial Engineering and Bio/Molecular Informatics Center, Konkuk University) ;
  • Lee, Sang-Hoo (Department of Microbial Engineering and Bio/Molecular Informatics Center, Konkuk University) ;
  • Park, Hey-Lin (Department of Microbial Engineering and Bio/Molecular Informatics Center, Konkuk Universityv) ;
  • Kwon, Tae-Jong (Department of Microbial Engineering and Bio/Molecular Informatics Center, Konkuk University) ;
  • Jung, Seun-Ho (Department of Microbial Engineering and Bio/Molecular Informatics Center, Konkuk University)
  • Published : 2002.06.01
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Abstract

A very rapid and efficient separation technique for cellular rhizobial cyclosophoraoses was developed based on fractional precipitation and partition chromatography. Cyclosophoraoses are known to function in the osmotic regulation and root nodule formation of legumes during the nitrogen fixation process. Cyclosophoraoses are produced as unbranched cyclic (1longrightarrow12)-${\beta}$-D-glucans in Agrobacterium or Rhizobium species. Recent research has shown that cyclosophoraoses can form inclusion complexation with various unstable or insoluble guest chemicals, thereby implying great potential for industrial application. Typical separation of pure cellular cyclosophoraoses has been so far carried out by several time-consuming steps, including size exclusion, anion exchange, and desalting liquid chromatographies, with a relatively poor recovery. However, the proposed method demonstrated that the successive application of fractional ethanol precipitation and one step of silica gel-based flash column chromatography was enough to simultaneously purify neutral or anionic forms of cyclosophoraoses. This novel technique is very rapid and provides a high recovery.

Keywords

References

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