Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Isolation and Biological Properties of Novel Cell Cycle Inhibitor, HY558, Isolated from Penicillium minioluteum F558

  • Lee, Chul-Hoon (Department of Medical Genetics & Institute of Biomedical Science, College of Medicine, Hanyang University) ;
  • Lim, Hae-Young (Department of Medical Genetics & Institute of Biomedical Science, College of Medicine, Hanyang University) ;
  • Kim, Min-Kyoung (Department of Medical Genetics & Institute of Biomedical Science, College of Medicine, Hanyang University) ;
  • Cho, Youl-Hee (Department of Medical Genetics & Institute of Biomedical Science, College of Medicine, Hanyang University) ;
  • Oh, Deok-Kun (Department of Bioscience and Biotechnology, Sejong University) ;
  • Kim, Chang-Jin (Korea Research Institute of Bioscience and Biotechnology) ;
  • Lim, Yoon-Gho (Bio/Molecular Informatics Center, Department of Applied Biology & Chemistry, Konkuk University)
  • Published : 2002.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

In the course of screening for a novel cell cycle inhibitor, a potent Cdk 1 inhibitor, HY558, was found from the culture broth of Penicillium minioluteum F558 isolated from a soil sample. The molecular ion of HY558 was identified at m/z 329 (MH+) with a molecular formula of $C_20H_44ON_2$. HY558 exhibited selective antiproliferative effects on various human cancer cell lines. Its $IC_50$ values were estimated to be 0.29 mM on HepG2, 0.30 mM on HeLa, 0.30 mM on HL6O, 0.33 mM on HT-29, and 0.25 mM on AGS cells. Interestingly, Hy558 demonstrated no antiproliferative effect with normal lymphocytes used as the control, and a low level of inhibition on the proliferation of A549 cancer cells. A flow cytometric analysis of HepG2 cells revealed an appreciable arrest of cells at the G1 and G2/M phases of the cell cycle following treatment with Hy558. furthermore, DNA fragmentation due to apoptosis was observed in HeLa cells treated with 0.46 mM of HY558.

Keywords

References

  1. J. Am. Chem. Soc. v.108 ¹H and (TEX)$^13C$(/TEX) assignments from sensitivity-enganced detection of heteronuclear multiple-bond connectivity by 2D multiple quantum NMR Bax, A.;M. Summers https://doi.org/10.1021/ja00268a061
  2. J. Magn. Reson. v.43 A simple method for suppressing dispersion-mode contributions in NMR spectra: The "pseudo echo" Bax, A.;R. Freeman
  3. J. Magn. Reson. v.55 Correlation of proton and nitrogen-15 chemical shifts by multiple quantum NMR Bax, A.;R. Freeman
  4. Anticancer Res. v.12 Screening for antimitotic compounds using the cdc25 tyrosine phosphatase, an activator of the mitosis-inducing P34 cdc2/cyclin B cdc 13 protein kinase Baratte, B.;:L. Meijer;K. Galaktinov;D. Beach
  5. J. Maga. Reson. v.48 Distortionless enhancement of NMR signals by polarization transfer Dodrell, D. M.;D. T. Pegg.;M. R. Bendall.
  6. Anal. Chem. v.28 Colorimeteric method for determination of sugars and related substance Dubois, M.;K. A. Gilles;J. K. Hamilton https://doi.org/10.1021/ac60111a017
  7. Science v.266 Cell cycle control and cancer Hartwell, L. H.;M. B. Kastan
  8. Mammalian Protein Metabolism Evolution of protein molecules Jukes, T. H.;C. R. Cantor
  9. J. Immunol. Methods v.65 Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays Mosmann, T. https://doi.org/10.1016/0022-1759(83)90303-4
  10. Annu. Rev. Biochem. v.61 Animal cell cycles and their control Norbury, C.;P. Nurse https://doi.org/10.1146/annurev.bi.61.070192.002301
  11. J. Microbiol. Biotechnol. v.11 In vitro detection of apoptosis in human promyelocytic leukemia HL-60 cells by ¹H-NMR. Lee, C.-H.;M. Lee;Y. Cho;H. Lim;J. Jung;K. H. Kim;Y. Lim
  12. J. Microbiol. Biotechnol. v.11 Flow cytometric analysis of silkworm hemolymph of the vaculovirus-induced insect cell spoptosis Rhee, W. J.;T. H. Park
  13. Trends Biochem. Sci. v.266 Cyclins and cyclin-dependent kinases: Take your parners Pines, J.
  14. Biochem. J. v.308 Cyclins and cyclin-dependent kinases: A biochemical view Pines, J. https://doi.org/10.1042/bj3080697
  15. Mol. Biol. Evol. v.4 The neighbor-joining method: A New method for reconstrucing phylogenic trees Saitou, N.;M. Mei