Journal of Microbiology and Biotechnology

  • 제12권3호
  • /
  • Pages.417-422
  • /
  • 2002
  • /
  • 1017-7825(pISSN)
  • /
  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Detection of Aromatic Pollutants by Bacterial Biosensors Bearing Gene Fusions Constructed with the dnaK Promoter of Pseudomonas sp. DJ-12

  • Park, Sang-Ho (Department of Microbiology and Biotechnology, and Biotechnology Research Institute, Chungbuk National University) ;
  • Lee, Dong-Hun (Department of Microbiology and Biotechnology, and Biotechnology Research Institute, Chungbuk National University) ;
  • Oh, Kye-Heon (Department of Life Science, Soonchunhyang University) ;
  • Lee, Kyoung (Department of Microbiology, Changwon National University) ;
  • Kim, Chi-Kyung (Department of Microbiology and Biotechnology, and Biotechnology Research Institute, Chungbuk National University)
  • 발행 : 2002.06.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Gene fusions were constructed by the transcriptional fusion of the dnaK promoter of pseudomonas sp. DJ-12 or E. coli to the lux or luc marker gene. The dnaKp-DJ::luxCDABE bioluminescent fusion in the biosensor using the Pseudomonas sp. DJ-12 dnaK promoter exhibited about 5-fold more extensive response to ethanol than that of dnaKp-EC::luxCDABE. The bioluminescent response of the dnaK-DJ::luc fusion to ethanol was much weaker than those of the other fusions. The biosensor harboring the dnaKp-DJ::luCDABE fusion was examined for its bioluminescence production based on exposure to aromatic compounds, such as biphenyl, 4-chlorobiphenyl (4CB), 4-hydroxybenzoate (4HBA), and catechol. In particular, the bioluminescence produced by the dnaKp-DJ::luxCDABE fusion was most sensitive to 1 mM biphenyl and 4CB when exposed for 80 min, and the responses were also very strong to other aromatics. Therefore, the biosensor bearing the dnaKp-DJ::luxCDABE fusion would appear to be the most useful for the detection of aromatics and other pollutants.

키워드

참고문헌

  1. Appl. Environ. Microbiol. v.64 A chromosomally based tod-luxCDABEwhole-cell reporter for benzene, toluene, ethylbenzene and xylene (BTEX) sensing Applegate, B. M.;S. R. Kehrmeyer;G. Sayler
  2. Appl. Environ. Microbiol. v.62 Oxidative stress detection with Escherichia cole harboring a katG'::lux fusion Belkin, S.;D. R. Smulski;A. C. Vollmer;T. K. Van Dyk;R. A. LaRossa
  3. Appl. Environ. Microbiol. v.58 Unique and overlapping pollutant stress proteins of Escherichia coli Blom, A.;W. Harder;A. Matin
  4. Appl. Environ. Microbiol. v.65 Green fluorescent protein as a noninvasive stress probe in resting Escherichia coli cells Cha, H. J.;R. Srivastava;V. N. Vakhria;G. Rao;W. Bentaley
  5. Proc. Natl. Acad. Sci. v.82 no.9 Consensus sequence for Escherichia coli heat shock gene promoters Cowing, D. W.;J. C. Barkwell;E. A. Craig;C. Woolford;R. W. Hendrix;C. A. Gross https://doi.org/10.1073/pnas.82.9.2679
  6. FEMS Microbiol. Lett. v.190 Detection and quanification of tetracyclines by whole cell biosensors Hansen, L. H.;S. J. Sorensen https://doi.org/10.1111/j.1574-6968.2000.tb09298.x
  7. Appl. Environ. Microbiol. v.60 Optical biosensor for environmental on-line monitoring of naphthalene and salicylate bioavailability with an immobilized bioluminescent catabolic reporter bacterium Hertzer, A.;K. Malachowsky;J. E. Thonnard;P.R. Bienkowski;D. C. White;G. S. Saylar
  8. J. Bacteriol. v.171 Escherichia cole DnaK and GrpE heat shock proteins interact both in vivo and in vitro Johnsom, C.;G. N. Chandrasekher;C. Georgopoulos https://doi.org/10.1128/jb.171.3.1590-1596.1989
  9. Appl. Environ. Microbiol. v.62 Genetic structure of the gene encoding 2,3-DHBP dioxygenase and HOPDA hydrolase from biphenyl- and 4CB-degrading Pseudomonas sp. stain DJ-12 Kim, E.;Y. Kim;C. K. Kim
  10. Science v.249 Rapid, sensitive bioluminescent reporter technology for naphthalene exposure and biodegradation King, J. M. H.;P. M. Digrazia;B. Applegate;R. Burlage;J. Sanseverino;P. Dunbar;F. Larimer;G. S. Sayler https://doi.org/10.1126/science.249.4970.778
  11. J. Microbiol. Biotechnol. v.9 Responses of Pseudomonas sp. DJ-12 to pollutant stresses of benzoate and 4-dhlorobenzoate Ko, Y.J.;S. H. Park;C. K. Kim
  12. Appl. Environ. Microbiol. v.64 Construction of bioluminescent riporter strain to detect polychlorinated biphenyls Layton, A. C.;M. Muccini;M. M. Ghosh;G. S. Sayler
  13. American Society for Microbiology Eschericia coli and Salmonelle typhimurium: Cellular and Molecular Biology Neidhardt, F. C.;R. A. Vanbogelen
  14. Curr. Microbiol. v.43 Adaptive and cross-protective responses of Pseudomonas sp. DJ-12 to several aromatics and other stress shocks Park, S. H.;Y. J. Ko;K. H. Oh;C. K. Kim https://doi.org/10.1007/s002840010283
  15. J. Microbiol. v.36 Cellular response of Pseudomonas sp. DJ-12 to the strisses of several aromatics pollutants Park, S. H.;Y. J. Ko;K. H. Oh;C. K. Kim
  16. J. Microbiol. v.36 Production of stress-shock proteins in Pseudomonas sp. DJ-12 treated with 4-hydroxybenzoate Park, S. H.;K. H. Oh;K. J. Lee;C. K. Kim
  17. Ecotoxi. Environ. Safe. v.33 Innate immune function as a bioindicator of pollution stress in fish Rice, C. D.;D. H. Kergosien;S. M. Adams https://doi.org/10.1006/eesa.1996.0024
  18. J. Bacteriol. v.169 Regulation of the vir genes of Agrobacterium tumefaciens plasmid pTiC58 Rogowsky, P. M.;T. J. Close;J. A. Chimera;J. J. Shaw;C. I. Kado https://doi.org/10.1128/jb.169.11.5101-5112.1987
  19. Molecular Cloning, 2nd ed. Sambrook, J.;E. F. Fritsch;T. Maniatis
  20. Appl. Environ. Microbiol. v.63 Recombinant luminescent bacteria for measuring bioavailable arsenite and antimonite Tauriainen, S.;M. Virta;W. Chang;M. Karp
  21. Anal. Biochem. v.272 Measurement of firefly luciferase reporter gene activity from cells and lysates using Escherichia cole arsenite and mercury sensors Taruiainen, S.;M. Virta;W. Chang;M. Karp https://doi.org/10.1006/abio.1999.4193
  22. Toxicol. Lett. v.102-103 Neurotoxicity of environmental chemicals and their mechanism of action Tilson, H. A.;P. R. S. Kodavanti;W. R. Mundy;P. J. Bushnell https://doi.org/10.1016/S0378-4274(98)00271-9
  23. Appl. Environ. Microbiol. v.60 Rapid and sensitive pollutant detection by induction of heat shock gene-bioluminescence gene fusions Van Dyk, T. K.;W. R. Najarian;K. B. Konstantinov;R. M. Young;P. S. Khurjati;R. A. LaRossa
  24. Appl. Environ. Microbiol. v.61 Responses to toxicants of an Escherichia coli strain carrying a uspA'::lux genetic fusing and an E. coli strain carrying a grpE'::lux fusion are similar Van Dyk, T. K.;D. R. Smulski;T. R. Reed;S. Belkin;A. C. Vollmer;R. A. LaRossa
  25. Appl. Environ. Microbiol. v.64 Development and testing of a bacterial biosensor for toluene-based environmental contaminants Willardson, B. M.;J. F. Wilkins;T. A. Rand;J. A. Rand;J. M. Schupp;K. K. Hill;P. Keim;P. J. Jackson