Journal of Microbiology and Biotechnology

  • 제12권3호
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  • Pages.382-388
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  • 2002
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

FAME Analysis to Monitor Impact of Organic Matter on Soil Bacterial Populations

  • Kim, Jong-Shik (Department of Applied Biology and Chemistry, Konkuk University) ;
  • Joo, Jin-Bee (Department of Applied Biology and Chemistry, Konkuk University) ;
  • Weon, Hang-Yeon (National Institute of Agricultural Science and Technology) ;
  • Kang, Chang-Seong (Kyonggi Province Agricultural Research and Extension Services) ;
  • Lee, Si-Kyung (Department of Applied Biology and Chemistry, Konkuk University) ;
  • Yahng, Chahng-Sool (Department of Applied Biology and Chemistry, Konkuk University)
  • 발행 : 2002.06.01
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초록

In order to assess the effects of organic fertilizer on soil microbial community structure and diversity in the greenhouse fields, fatty acid methyl ester (FAME) was analyzed by the MIDI (Microbial ID, Inc., Newark, DE, U.S.A.) system and enumerations were performed. In relation to bacterial division of each sample, low GC Gram-positive bacteria were predominant among bacteria cultured on aerobic bacteria media. On the other hand, alpha subdivision was predominant on proteobacteria of control and OM (organic matter) 1 treated plot, and Flavobacterium spp. existed in OM2 plot on crystal violet media of all samples. Shannon-weaver Index (H) of OM1 plot varied most by 1.9 and 5.0 among bacteria cultured on aerobic bacteria media and crystal violet media, respectively. Our results revealed that addition of the organic wastes to soil led to a highly diverse microbial community, but the excessive amounts of organic and mineral fertilizer applied in the greenhouse fields produced excess nutrients in soil and led to simplification on bacterial populations.

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